LOCUS       V028657                10236 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028657
VERSION     V028657
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2279..2288
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             2288..2359
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags"
                     /transl_table=1
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
                     /label="3xFLAG"
     CDS             2366..4345
                     /label="PABPC4(NM_001135653)"
                     /note="PABPC4(NM_001135653)"
                     /gene="PABPC4"
     promoter        4395..4606
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4619..4887
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4916..4925
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4946..5608
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5678..5731
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5732..6331
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6332..6919
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6993..7226
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7298..7419
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7438..7573
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7611..7627)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7635..7651
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7659..7689)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7704..7725
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8013..8601)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8772..9632)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9633..9737)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10211..10227
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 caccatggac tacaaggatg acgatgacaa ggattacaaa gacgacgatg ataaggacta
     2341 taaggatgat gacgacaaag aattcatgaa cgctgcggcc agcagctacc ccatggcctc
     2401 cctgtacgtg ggcgacctgc attcggacgt caccgaggcc atgctgtacg aaaagttcag
     2461 ccccgcgggg cctgtgctgt ccatccgggt ctgccgcgat atgatcaccc gccgctccct
     2521 gggctatgcc tacgtcaact tccagcagcc ggccgacgct gagcgggctt tggacaccat
     2581 gaactttgat gtgattaagg gaaagccaat ccgcatcatg tggtctcaga gggatccctc
     2641 tttgagaaaa tctggtgtgg gaaacgtctt catcaagaac ctggacaaat ctatagataa
     2701 caaggcactt tatgatactt tttctgcttt tggaaacata ctgtcctgca aggtggtgtg
     2761 tgatgagaac ggctctaagg gttatgcctt tgtccacttc gagacccaag aggctgccga
     2821 caaggccatc gagaagatga atggcatgct cctcaatgac cgcaaagtat ttgtgggcag
     2881 attcaagtct cgcaaagagc gggaagctga gcttggagcc aaagccaagg aattcaccaa
     2941 tgtttatatc aaaaactttg gggaagaggt ggatgatgag agtctgaaag agctattcag
     3001 tcagtttggt aagaccctaa gtgtcaaggt gatgagagat cccaatggga aatccaaagg
     3061 ctttggcttt gtgagttacg aaaaacacga ggatgccaat aaggctgtgg aagagatgaa
     3121 tggaaaagaa ataagtggta aaatcatatt tgtaggccgt gcacaaaaga aagtagaacg
     3181 gcaggcagag ttaaaacgga aatttgaaca gttgaaacag gagagaatta gtcgatatca
     3241 gggggtgaat ctctacatta agaacttgga tgacactatt gatgatgaga aattaaggaa
     3301 agaattttct ccttttggat caattaccag tgctaaggta atgctggagg atggaagaag
     3361 caaagggttt ggcttcgtct gcttctcatc tcctgaagaa gcaaccaaag cagtcactga
     3421 gatgaatgga cgcattgtgg gctccaagcc actatatgtt gccctggccc agaggaagga
     3481 agagagaaag gctcacctga ccaaccagta tatgcaacga gtggctggaa tgagagcact
     3541 tcctgccaat gccatcttaa atcagttcca gcctgcagcg ggtggctact ttgtgccagc
     3601 agtcccacag gctcagggaa ggcctccata ttatacacct aaccagttag cacagatgag
     3661 gcctaatcca cgctggcagc aaggtgggag acctcaaggc ttccaaggaa tgccaagtgc
     3721 tatacgccag tctgggcctc gtccaactct tcgccatctg gctccaactg gtaatgctcc
     3781 ggcctctcgt ggcctcccta ctaccactca gagagtcggg tctgagtgcc cggaccgctt
     3841 ggctatggac tttggtgggg ctggtgccgc ccagcaaggg ctgactgaca gctgccagtc
     3901 tggaggcgtt cccacagctg tgcagaactt agcgccacgc gctgctgttg ctgctgctgc
     3961 tccccgggct gttgccccct acaaatacgc ctccagtgtc cgcagccctc atcctgccat
     4021 acagcctctg caggcacccc agcctgcggt ccatgtgcag gggcaggagc cactgactgc
     4081 ctccatgctg gctgcagcac ccccccagga acagaagcag atgctgggag aacgcttgtt
     4141 cccactcatc caaacaatgc attcaaatct ggctgggaag atcacgggaa tgctgctgga
     4201 gatagacaac tctgagctgc tgcacatgtt agagtccccc gagtctctcc gctccaaggt
     4261 ggatgaagct gtagcagttc tacaggctca tcatgccaag aaagaagctg cccagaaggt
     4321 gggcgctgtt gctgctgcta cctcttaggg atccgcggcc gcaaggatct gcgatcgctc
     4381 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     4441 ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     4501 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     4561 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg
     4621 ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt
     4681 gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt
     4741 aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag
     4801 actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc
     4861 gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac
     4921 catggagagc gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac
     4981 cctgaacggc gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg
     5041 catgaccaac aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag
     5101 ccacgtgatg ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc
     5161 cttcctgcac gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga
     5221 cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga
     5281 cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat
     5341 ccgcagcaac gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag
     5401 cttcgcccgc accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca
     5461 catgcacttc aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc
     5521 cttccgccgc gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca
     5581 cgccttcaag acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt
     5641 ggacggcacc gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac
     5701 atgcggtgac gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct
     5761 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     5821 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     5881 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     5941 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     6001 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     6061 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     6121 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     6181 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     6241 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     6301 ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga
     6361 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     6421 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     6481 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     6541 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     6601 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     6661 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     6721 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     6781 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     6841 gctgccggct ctgcggcctc ttccgcgtct ccgccttcgc cctcagacga gtcggatctc
     6901 cctttggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga
     6961 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaa
     7021 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     7081 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7141 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7201 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     7261 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     7321 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     7381 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     7441 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     7501 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     7561 ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg
     7621 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     7681 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     7741 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     7801 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     7861 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     7921 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     7981 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     8041 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     8101 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     8161 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     8221 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     8281 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     8341 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     8401 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     8461 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     8521 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     8581 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8641 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8701 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8761 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     8821 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8881 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     8941 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9001 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9061 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     9121 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9181 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9241 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     9301 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     9361 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9421 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9481 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9541 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9601 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9661 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9721 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     9781 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     9841 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     9901 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     9961 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
    10021 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca
    10081 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
    10141 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
    10201 tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
