LOCUS V036158 9379 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V036158 VERSION V036158 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..4209 /label="PABPC1(NM_002568)" /note="PABPC1(NM_002568)" /gene="PABPC1" CDS 4216..4305 /codon_start=1 /product="3 tandem Myc epitope tags" /transl_table=1 /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" /label="3xMyc" promoter 4349..4560 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4573..4841 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 4868..5467 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5474..6062 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6136..6369 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6441..6562 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6581..6716 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6754..6770) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6778..6794 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6802..6832) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6847..6868 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7156..7744) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7915..8775) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8776..8880) /gene="bla" /label="AmpR promoter" primer_bind 9354..9370 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catgaacccc agtgccccca gctaccccat ggcctcgctc 2341 tacgtggggg acctccaccc cgacgtgacc gaggcgatgc tctacgagaa gttcagcccg 2401 gccgggccca tcctctccat ccgggtctgc agggacatga tcacccgccg ctccttgggc 2461 tacgcgtatg tgaacttcca gcagccggcg gacgcggagc gtgctttgga caccatgaat 2521 tttgatgtta taaagggcaa gccagtacgc atcatgtggt ctcagcgtga tccatcactt 2581 cgcaaaagtg gagtaggcaa catattcatt aaaaatctgg acaaatccat tgataataaa 2641 gcactgtatg atacattttc tgcttttggt aacatccttt catgtaaggt ggtttgtgat 2701 gaaaatggtt ccaagggcta tggatttgta cactttgaga cgcaggaagc agctgaaaga 2761 gctattgaaa aaatgaatgg aatgctccta aatgatcgca aagtatttgt tggacgattt 2821 aagtctcgta aagaacgaga agctgaactt ggagctaggg caaaagaatt caccaatgtt 2881 tacatcaaga attttggaga agacatggat gatgagcgcc ttaaggatct ctttggcaag 2941 tttgggcctg ccttaagtgt gaaagtaatg actgatgaaa gtggaaaatc caaaggattt 3001 ggatttgtaa gctttgaaag gcatgaagat gcacagaaag ctgtggatga gatgaacgga 3061 aaggagctca atggaaaaca aatttatgtt ggtcgagctc agaaaaaggt ggaacggcag 3121 acggaactta agcgcaaatt tgaacagatg aaacaagata ggatcaccag ataccagggt 3181 gttaatcttt atgtgaaaaa tcttgatgat ggtattgatg atgaacgtct ccggaaagag 3241 ttttctccat ttggtacaat cactagtgca aaggttatga tggagggtgg tcgcagcaaa 3301 gggtttggtt ttgtatgttt ctcctcccca gaagaagcca ctaaagcagt tacagaaatg 3361 aacggtagaa ttgtggccac aaagccattg tatgtagctt tagctcagcg caaagaagag 3421 cgccaggctc acctcactaa ccagtatatg cagagaatgg caagtgtacg agctgttccc 3481 aaccctgtaa tcaaccccta ccagccagca cctccttcag gttacttcat ggcagctatc 3541 ccacagactc agaaccgtgc tgcatactat cctcctagcc aaattgctca actaagacca 3601 agtcctcgct ggactgctca gggtgccaga cctcatccat tccaaaatat gcccggtgct 3661 atccgcccag ctgctcctag accaccattt agtactatga gaccagcttc ttcacaggtt 3721 ccacgagtca tgtcaacaca gcgtgttgct aacacatcaa cacagacaat gggtccacgt 3781 cctgcagctg cagccgctgc agctactcct gctgtccgca ccgttccaca gtataaatat 3841 gctgcaggag ttcgcaatcc tcagcaacat cttaatgcac agccacaagt tacaatgcaa 3901 cagcctgctg ttcatgtaca aggtcaggaa cctttgactg cttccatgtt ggcatctgcc 3961 cctcctcaag agcaaaagca aatgttgggt gaacggctgt ttcctcttat tcaagccatg 4021 caccctactc ttgctggtaa aatcactggc atgttgttgg agattgataa ttcagaactt 4081 cttcatatgc tcgagtctcc agagtcactc cgttctaagg ttgatgaagc tgtagctgta 4141 ctacaagccc accaagctaa agaggctgcc cagaaagcag ttaacagtgc caccggtgtt 4201 ccaactgttg gatccgaaca aaaactaata tcagaggaag atttggaaca gaaactaatc 4261 agtgaagaag atttagagca aaagttaatt tccgaggagg acttatagcg gccgcgaagg 4321 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag 4381 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac 4441 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat 4501 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag 4561 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc 4621 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg 4681 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct 4741 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc 4801 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct 4861 acgctagatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg 4921 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga 4981 cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg 5041 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac 5101 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt 5161 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc 5221 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg 5281 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc 5341 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt 5401 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg 5461 tgcctgagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct 5521 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc 5581 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct 5641 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga 5701 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc 5761 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 5821 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt 5881 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt 5941 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc 6001 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc 6061 gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa 6121 aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat ctgctttttg 6181 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag 6241 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc 6301 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 6361 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat 6421 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca 6481 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 6541 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc 6601 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 6661 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga 6721 cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg tgtgaaattg 6781 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 6841 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 6901 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 6961 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 7021 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 7081 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 7141 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 7201 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 7261 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 7321 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 7381 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 7441 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 7501 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 7561 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 7621 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 7681 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 7741 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 7801 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 7861 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 7921 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 7981 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 8041 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 8101 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 8161 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 8221 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 8281 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 8341 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 8401 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 8461 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 8521 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 8581 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 8641 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 8701 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 8761 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 8821 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 8881 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac 8941 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga 9001 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg 9061 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa 9121 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca 9181 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg 9241 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg 9301 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac 9361 gacggccagt gccaagctg //