LOCUS       V045078                 9952 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045078
VERSION     V045078
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2279..2288
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             2288..2359
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags"
                     /transl_table=1
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
                     /label="3xFLAG"
     CDS             2366..2422
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             2429..4060
                     /label="P4HA3(NM_182904)"
                     /note="P4HA3(NM_182904)"
                     /gene="P4HA3"
     promoter        4111..4322
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4335..4603
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4632..4641
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4662..5324
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5394..5447
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5448..6047
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6048..6635
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6709..6942
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7014..7135
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7154..7289
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7327..7343)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7351..7367
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7375..7405)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7420..7441
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7729..8317)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8488..9348)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9349..9453)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9927..9943
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 caccatggac tacaaggatg acgatgacaa ggattacaaa gacgacgatg ataaggacta
     2341 taaggatgat gacgacaaag aattcgccac taacttctcc ctgttgaaac aagcagggga
     2401 tgtcgaagag aatcccgggc caccggtcat gggtcctggg gcgcggctgg cggcgctgct
     2461 ggcggtgctg gcgctcggga caggagaccc agaaagggct gcggctcggg gcgacacgtt
     2521 ctcggcgctg accagcgtgg cgcgcgccct ggcgcccgag cgccggctgc tggggctgct
     2581 gaggcggtac ctgcgcgggg aggaggcgcg gctgcgggac ctgactagat tctacgacaa
     2641 ggtactttct ttgcatgagg attcaacaac ccctgtggct aaccctctgc ttgcatttac
     2701 tctcatcaaa cgcctgcagt ctgactggag gaatgtggta catagtctgg aggccagtga
     2761 gaacatccga gctctgaagg atggctatga gaaggtggag caagaccttc cagcctttga
     2821 ggaccttgag ggagcagcaa gggccctgat gcggctgcag gacgtgtaca tgctcaatgt
     2881 gaaaggcctg gcccgaggtg tctttcagag agtcactggc tctgccatca ctgacctgta
     2941 cagccccaaa cggctctttt ctctcacagg ggatgactgc ttccaagttg gcaaggtggc
     3001 ctatgacatg ggggattatt accatgccat tccatggctg gaggaggctg tcagtctctt
     3061 ccgaggatct tacggagagt ggaagacaga ggatgaggca agtctagaag atgccttgga
     3121 tcacttggcc tttgcttatt tccgggcagg aaatgtttcg tgtgccctca gcctctctcg
     3181 ggagtttctt ctctacagcc cagataataa gaggatggcc aggaatgtct tgaaatatga
     3241 aaggctcttg gcagagagcc ccaaccacgt ggtagctgag gctgtcatcc agaggcccaa
     3301 tataccccac ctgcagacca gagacaccta cgaggggcta tgtcagaccc tgggttccca
     3361 gcccactctc taccagatcc ctagcctcta ctgttcctat gagaccaatt ccaacgccta
     3421 cctgctgctc cagcccatcc ggaaggaggt catccacctg gagccctaca ttgctctcta
     3481 ccatgacttc gtcagtgact cagaggctca gaaaattaga gaacttgcag aaccatggct
     3541 acagaggtca gtggtggcat caggggagaa gcagttacaa gtggagtacc gcatcagcaa
     3601 aagtgcctgg ctgaaggaca ctgttgaccc aaaactggtg accctcaacc accgcattgc
     3661 tgccctcaca ggccttgatg tccggcctcc ctatgcagag tatctgcagg tggtgaacta
     3721 tggcatcgga ggacactatg agcctcactt tgaccatgct acgtcaccaa gcagccccct
     3781 ctacagaatg aagtcaggaa accgagttgc aacatttatg atctatctga gctcggtgga
     3841 agctggagga gccacagcct tcatctatgc caacctcagc gtgcctgtgg ttaggaatgc
     3901 agcactgttt tggtggaacc tgcacaggag tggtgaaggg gacagtgaca cacttcatgc
     3961 tggctgtcct gtcctggtgg gagataagtg ggtggccaac aagtggatac atgagtatgg
     4021 acaggaattc cgcagaccct gcagctccag ccctgaagac tgaggatccg cggccgcgaa
     4081 ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
     4141 agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa
     4201 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
     4261 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
     4321 agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc
     4381 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg
     4441 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc
     4501 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac
     4561 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc
     4621 ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg gagatcgagt
     4681 gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc ggagagggca
     4741 cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc ctgaccttca
     4801 gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc acctacccca
     4861 gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc aacacccgca
     4921 tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg
     4981 gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac agcgtgatct
     5041 tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc atgggcgata
     5101 acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc tactacagct
     5161 tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc ctgcagaacg
     5221 ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc gagctgggca
     5281 tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt
     5341 cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct cgcgagggca
     5401 gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg accgagtaca
     5461 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     5521 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     5581 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     5641 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     5701 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     5761 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     5821 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     5881 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     5941 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     6001 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg
     6061 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     6121 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     6181 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     6241 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     6301 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     6361 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     6421 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     6481 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     6541 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctccgc cttcgccctc
     6601 agacgagtcg gatctccctt tggccgcctc cccgcctggt acctttaaga ccaatgactt
     6661 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     6721 ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     6781 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     6841 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     6901 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     6961 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     7021 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     7081 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     7141 tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7201 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     7261 gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta
     7321 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7381 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     7441 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     7501 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     7561 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     7621 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     7681 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7741 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7801 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7861 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7921 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7981 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8041 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8101 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8161 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8221 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8281 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8341 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8401 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8461 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8521 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     8581 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     8641 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8701 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8761 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     8821 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8881 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8941 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9001 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9061 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9121 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9181 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9241 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9301 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9361 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9421 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     9481 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     9541 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     9601 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     9661 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     9721 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     9781 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     9841 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     9901 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//
