LOCUS       V033540                10810 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033540
VERSION     V033540
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4836
                     /label="Otud7b(NM_001025613)"
                     /note="Otud7b(NM_001025613)"
                     /gene="Otud7b"
     CDS             4843..4908
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4952..5163
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5176..5444
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5473..5482
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5503..6165
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6235..6288
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6289..6888
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6889..7477
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7551..7784
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7856..7977
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8017..8152
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8185..8201)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8209..8225
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8233..8263)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8278..8299
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8587..9175)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9346..10206)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10207..10311)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10785..10801
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga ccctggacat ggatgctgtc
     2341 ctgtcggatt ttgttcgttc cacaggtgca gagccagggc tggctcgaga tcttctagaa
     2401 ggaaagaact gggatgtgag tgctgccctc agtgattttg aacagttgcg tcaagtccat
     2461 gctgggaacc tatccccacc ctttagtgga gggagtactt gccctaagac ccctgaaaaa
     2521 gggggttcgg atagagagcc cactcgccct tctcgaccca tccttcagag acaagatgac
     2581 gtcattcaag aaaaacgcct gtctaggggc atctcccacg ccagctccag cattgtttcc
     2641 ctggcccgct cccatgtctc ctccaatggt gggggtgggg ggagcagtga gcaccccctg
     2701 gaaatgccca tctgtgcctt ccagcttcca gatctcactg tgtacaaaga agacttccgc
     2761 agcttcatag agcgagacct cattgagcag tccatgctgg ttgccttgga acaggcaggg
     2821 cgtctgaatt ggtgggttag tatggactcc acctgccaga ggctgctccc tttagcaact
     2881 actggggatg ggaactgcct cctgcatgca gcatctcttg ggatgtgggg tttccatgat
     2941 agagacttgg tgctgaggaa agcgctgtat gcactgatgg agaagggggt ggagaaggaa
     3001 gcactgagaa ggcgctggag gtggcagcaa acacagcaga ataaagagtc aggactggtg
     3061 tatacggaag atgagtggca gaaggaatgg aatgaattga tcaaacttgc ttcaagtgaa
     3121 cccagaatgc atctgggttc caacggagcc agtggtggtg gagtggagag ctccgaggag
     3181 ccggtatatg aaagcctaga ggaattccat gtcttcgtcc ttgctcatgt gctcaagagg
     3241 cccatagtgg tcgtggcaga caccatgctg agggactctg gaggggaagc atttgcccct
     3301 atcccatttg gagggatcta tctgccattg gaggttccag ccagccagtg tcaccgatcc
     3361 cctctggtgc tcgcctatga tcaggcccac ttctctgcgc ttgtgtcgat ggagcagaag
     3421 gagagcgcca aagaacaagc tgtgatccca ctcacagatt cagagcacaa gctgctgccc
     3481 ctgcactttg ctgtggaccc aggaaagggc tgggagtggg gcaaagatga caatgacaat
     3541 gtccgattgg ccagtataat tctgtctctg gaggtgaagt tacatctgct gcatagctat
     3601 atgaatgtga agtggatccc actgtcttct gattcacaag ctcccctagc tcagcctgag
     3661 tcccccacag cctcagctgg agatgaaccc aggtccactc ctgagtctgg ggagtcagac
     3721 aaggagtcgg ttggcagcag ttctctcggc aacgagggca gcaggcggaa ggagaagtca
     3781 aagcgagacc gggagaagga caagaagaga gcagattccg tggctaacaa actgggcagc
     3841 tttggcaaaa ccttgggcag caagcttaag aagaacatgg ggggcctgat gcacagcaag
     3901 ggccccaagc ctggagggct ggggagtggg tctggaataa gcagtggtac ggagacatta
     3961 gaaaagaaga agaagaacaa cacattgaag agctggaagg gtggcaagga agaggctgct
     4021 ggggatgggc ctgtgtctga gaagcccccg tctgagtctg ttggcaatgg cgggagcaag
     4081 tatagccagg aggtgatgca aagcctgagc actatgagga tcgcaatgca aggggagggc
     4141 aagtacattt ttgttggaac cctgaagatg ggccaccggc accagtatca ggaggagatg
     4201 atccagcgtt accttgcaga tgctgaggaa agattcctgg cagagcagaa gcagaaggag
     4261 gttgagagga agatcatgaa tggagggtta gttagtgggc ctcctccagc caaaaagcca
     4321 gagccggacg gtggggagga ccagcccagt gactcccccg cagagcccaa ggcgatggcc
     4381 ttctctactg cttaccctgg gggctttact atccctcggc cttctggggg tggagtccac
     4441 tgccaggaac cccggaggca attggcaggg gggccatgtg tagggggctt accatcatat
     4501 gccacctttc ccagacagta tcctgggcga ccgtaccccc accaggacaa catcccagct
     4561 ctggagccag gcaaagatgg agttcacagg ggtgccttgt taccacccca gttccgtgtg
     4621 gctgattcct atagcaatgg ctacagagag cccccagagc cagatggatg ggctggagct
     4681 ccacggggac ttcccccaac ccagaccaaa tgcaaacaac cgaactgcag cttctatgga
     4741 caccctgaga caaacaactt gtgctcttgc tgttacaggg aagaactgag gcggagggag
     4801 cgggaacctg gtggggaact gctggcacac aggttcggat ccgactacaa agaccatgac
     4861 ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta gcggccgcga
     4921 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     4981 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
     5041 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     5101 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     5161 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc
     5221 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     5281 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     5341 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
     5401 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg
     5461 cctacgctag acgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag
     5521 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc
     5581 acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc
     5641 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc
     5701 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc
     5761 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc
     5821 ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc
     5881 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat
     5941 aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc
     6001 ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac
     6061 gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc
     6121 atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag
     6181 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc
     6241 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac
     6301 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     6361 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     6421 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     6481 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     6541 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     6601 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     6661 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     6721 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     6781 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     6841 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg
     6901 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     6961 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     7021 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     7081 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     7141 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     7201 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     7261 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     7321 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     7381 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     7441 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa gaccaatgac
     7501 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     7561 aattcactcc caacgaagat aagatctgct ttttgcttgt actgggtctc tctggttaga
     7621 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7681 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7741 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     7801 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     7861 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7921 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7981 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     8041 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     8101 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt
     8161 tgcagagacc aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
     8221 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     8281 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     8341 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     8401 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8461 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8521 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8581 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8641 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8701 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8761 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8821 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8881 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8941 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9001 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     9061 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9121 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9181 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9241 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9301 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9361 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9421 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9481 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9541 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9601 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9661 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9721 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9781 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9841 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9901 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9961 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10021 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    10081 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10141 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    10201 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    10261 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    10321 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
    10381 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
    10441 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
    10501 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
    10561 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
    10621 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
    10681 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
    10741 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
    10801 tgccaagctg
//
