LOCUS       .                       8278 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        6..233
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    458..583
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1076..1309
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    1804..1920
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2010..2213
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2302..3459
                     /label="ovalbumin"
                     /note="chicken ovalbumin"
     CDS             3460..3486
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             3490..3516
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             3520..3546
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             3634..4341
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     misc_feature    4357..4945
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5019..5252
                     /label="3' LTR (ΔU3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5324..5458
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5485..5620
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     primer_bind     complement(5653..5669)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5677..5693)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5701..5731)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5746..5767)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6055..6643)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6817..7674)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(7675..7779)
                     /label="AmpR promoter"
     primer_bind     8253..8269
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgggctcc atcggcgcag caagcatgga attttgtttt
     2341 gatgtattca aggagctcaa agtccaccat gccaatgaga acatcttcta ctgccccatt
     2401 gccatcatgt cagctctagc catggtatac ctgggtgcaa aagacagcac caggacacag
     2461 ataaataagg ttgttcgctt tgataaactt ccaggattcg gagacagtat tgaagctcag
     2521 tgtggcacat ctgtaaacgt tcactcttca cttagagaca tcctcaacca aatcaccaaa
     2581 ccaaatgatg tttattcgtt cagccttgcc agtagacttt atgctgaaga gagataccca
     2641 atcctgccag aatacttgca gtgtgtgaag gaactgtata gaggaggctt ggaacctatc
     2701 aactttcaaa cagctgcaga tcaagccaga gagctcatca attcctgggt agaaagtcag
     2761 acaaatggaa ttatcagaaa tgtccttcag ccaagctccg tggattctca aactgcaatg
     2821 gttctggtta atgccattgt cttcaaagga ctgtgggaga aagcatttaa ggatgaagac
     2881 acacaagcaa tgcctttcag agtgactgag caagaaagca aacctgtgca gatgatgtac
     2941 cagattggtt tatttagagt ggcatcaatg gcttctgaga aaatgaagat cctggagctt
     3001 ccatttgcca gtgggacaat gagcatgttg gtgctgttgc ctgatgaagt ctcaggcctt
     3061 gagcagcttg agagtataat caactttgaa aaactgactg aatggaccag ttctaatgtt
     3121 atggaagaga ggaagatcaa agtgtactta cctcgcatga agatggagga aaaatacaac
     3181 ctcacatctg tcttaatggc tatgggcatt actgacgtgt ttagctcttc agccaatctg
     3241 tctggcatct cctcagcaga gagcctgaag atatctcaag ctgtccatgc agcacatgca
     3301 gaaatcaatg aagcaggcag agaggtggta gggtcagcag aggctggagt ggatgctgca
     3361 agcgtctctg aagaatttag ggctgaccat ccattcctct tctgtatcaa gcacatcgca
     3421 accaacgccg ttctcttctt tggcagatgt gtttcccctt acccatacga cgtacctgat
     3481 tatgcaactt atccttatga tgtacctgat tatgctagct atccttatga tgtcccagac
     3541 tacgcaggat ccgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc
     3601 ggcccttccg gaatggagag cgacgagagc ggcctggtga gcaagggcga ggaggataac
     3661 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     3721 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     3781 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     3841 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     3901 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     3961 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     4021 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     4081 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     4141 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     4201 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     4261 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     4321 ggcatggacg agctgtacaa gtagggatcc gtcgacaatc aacctctgga ttacaaaatt
     4381 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     4441 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     4501 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     4561 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     4621 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     4681 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     4741 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     4801 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     4861 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     4921 atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc
     4981 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     5041 acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     5101 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     5161 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     5221 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     5281 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     5341 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     5401 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     5461 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5521 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     5581 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagaccaa
     5641 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     5701 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     5761 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     5821 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     5881 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5941 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6001 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6061 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6121 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6181 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6241 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6301 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6361 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6421 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6481 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6541 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     6601 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     6661 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     6721 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     6781 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     6841 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     6901 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     6961 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7021 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7081 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     7141 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     7201 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     7261 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     7321 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     7381 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     7441 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     7501 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     7561 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     7621 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     7681 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     7741 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     7801 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     7861 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     7921 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     7981 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     8041 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     8101 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     8161 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     8221 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg
//
