LOCUS pLV3-CMV-OGT(hum 10606 bp DNA circular SYN 09-NOV-2022 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10606) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..10606 /mol_type="other DNA" /organism="synthetic DNA construct" LTR 234..414 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 458..583 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1076..1309 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." misc_feature 1804..1920 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2010..2213 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" regulatory 2296..2305 /label=Kozak sequence /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" CDS 2302..5439 /codon_start=1 /label=OGT /translation="MASSVGNVADSTEPTKRMLSFQGLAELAHREYQAGDFEAAERHCM QLWRQEPDNTGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYKERGQ LQEAIEHYRHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSALQYNPDLYCVRSDL GNLLKALGRLEEAKACYLKAIETQPNFAVAWSNLGCVFNAQGEIWLAIHHFEKAVTLDP NFLDAYINLGNVLKEARIFDRAVAAYLRALSLSPNHAVVHGNLACVYYEQGLIDLAIDT YRRAIELQPHFPDAYCNLANALKEKGSVAEAEDCYNTALRLCPTHADSLNNLANIKREQ GNIEEAVRLYRKALEVFPEFAAAHSNLASVLQQQGKLQEALMHYKEAIRISPTFADAYS NMGNTLKEMQDVQGALQCYTRAIQINPAFADAHSNLASIHKDSGNIPEAIASYRTALKL KPDFPDAYCNLAHCLQIVCDWTDYDERMKKLVSIVADQLEKNRLPSVHPHHSMLYPLSH GFRKAIAERHGNLCLDKINVLHKPPYEHPKDLKLSDGRLRVGYVSSDFGNHPTSHLMQS IPGMHNPDKFEVFCYALSPDDGTNFRVKVMAEANHFIDLSQIPCNGKAADRIHQDGIHI LVNMNGYTKGARNELFALRPAPIQAMWLGYPGTSGALFMDYIITDQETSPAEVAEQYSE KLAYMPHTFFIGDHANMFPHLKKKAVIDFKSNGHIYDNRIVLNGIDLKAFLDSLPDVKI VKMKCPDGGDNADSSNTALNMPVIPMNTIAEAVIEMINRGQIQITINGFSISNGLATTQ INNKAATGEEVPRTIIVTTRSQYGLPEDAIVYCNFNQLYKIDPSTLQMWANILKRVPNS VLWLLRFPAVGEPNIQQYAQNMGLPQNRIIFSPVAPKEEHVRRGQLADVCLDTPLCNGH TTGMDVLWAGTPMVTMPGETLASRVAASQLTCLGCLELIAKNRQEYEDIAVKLGTDLEY LKKVRGKVWKQRISSPLFNTKQYTMELERLYLQMWEHYAAGNKPDHMIKPVEVTESA" CDS 5446..5472 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 5476..5502 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 5506..5532 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" promoter 5576..5787 /label=EF-1-alpha core promoter /note="core promoter for human elongation factor EF-1-alpha" LTR 5800..6068 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" CDS 6095..6694 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 6701..7289 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" LTR 7363..7596 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 7668..7789 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin 7808..7943 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(7981..7997) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" rep_origin complement(8383..8971) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(join(9142..9933,9934..10002)) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(10003..10107) /gene="bla" /label=AmpR promoter primer_bind 10581..10597 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catggcgtct tccgtgggca acgtggccga cagcacagaa 2341 ccaacgaaac gtatgctttc cttccaaggg ttagctgagt tggcacatcg agaatatcag 2401 gcaggagatt ttgaggcagc tgagagacac tgcatgcagc tctggagaca agagccagac 2461 aatactggtg tgcttttatt actttcatct atacacttcc agtgtcgaag gctggacaga 2521 tctgctcact ttagcactct ggcaattaaa cagaaccccc ttctggcaga agcttattcg 2581 aatttgggga atgtgtacaa ggaaagaggg cagttgcagg aggcaattga gcattatcga 2641 catgcattgc gtctcaaacc tgatttcatc gatggttata ttaacctggc agccgccttg 2701 gtagcagcgg gtgacatgga aggggcagta caagcttacg tctctgctct tcagtacaat 2761 cctgatttgt actgtgttcg cagtgacctg gggaacctgc tcaaagccct gggtcgcttg 2821 gaagaagcca aggcatgtta tttgaaagca attgagacgc aaccgaactt tgcagtagct 2881 tggagtaatc ttggctgtgt tttcaatgca caaggggaaa tttggcttgc aattcatcac 2941 tttgaaaagg ctgtcaccct tgacccaaac tttctggatg cttatatcaa tttaggaaat 3001 gtcttgaaag aggcacgcat ttttgacaga gctgtggcag cttatcttcg tgccctaagt 3061 ttgagtccaa atcacgcagt ggtgcacggc aacctggctt gtgtatacta tgagcaaggc 3121 ctgatagatc tggcaataga cacctacagg cgggctatcg aactacaacc acatttccct 3181 gatgcttact gcaacctagc caatgctctc aaagagaagg gcagtgttgc tgaagcagaa 3241 gattgttata atacagctct ccgtctgtgt cccacccatg cagactctct gaataaccta 3301 gccaatatca aacgagaaca gggaaacatt gaagaggcag ttcgcttgta tcgtaaagca 3361 ttagaagtct tcccagagtt tgctgctgcc cattcaaatt tagcaagtgt actgcagcag 3421 cagggaaaac tgcaggaagc tctgatgcat tataaggagg ctattcgaat cagtcctacc 3481 tttgctgatg cctactctaa tatgggaaac actctaaagg agatgcagga tgttcaggga 3541 gccttgcagt gttatacgcg tgccatccaa attaatcctg catttgcaga tgcacatagc 3601 aatctggctt ccattcataa ggattcaggg aatattccag aagccatagc ttcttaccgc 3661 acggctctga aacttaagcc tgattttcct gatgcttatt gtaacttggc tcattgcctg 3721 cagattgtct gtgattggac agactatgat gagcgaatga agaagttggt cagtattgtg 3781 gctgaccagt tagagaagaa taggttgcct tctgtgcatc ctcatcatag tatgctatat 3841 cctctttctc atggcttcag gaaggctatt gctgagaggc acggcaacct gtgcttagat 3901 aagattaatg ttcttcataa accaccatat gaacatccaa aagacttgaa gctcagtgat 3961 ggtcggctgc gtgtaggata tgtgagttcc gactttggga atcatcctac ttctcacctt 4021 atgcagtcta ttccaggcat gcacaatcct gataaatttg aggtgttctg ttatgccctg 4081 agcccagacg atggcacaaa cttccgagtg aaggtgatgg cagaagccaa tcatttcatt 4141 gatctttctc agattccatg caatggaaaa gcagctgatc gcatccatca ggatggaatt 4201 catatccttg taaatatgaa tggctatact aagggcgctc gaaatgagct ttttgctctc 4261 aggccagctc ctattcaggc aatgtggctg ggataccctg ggacgagtgg tgcgcttttc 4321 atggattata ttatcactga tcaggaaact tcgccagctg aagttgctga gcagtattcc 4381 gagaaattgg cttatatgcc ccacactttt tttattggtg atcatgctaa tatgttccct 4441 cacctgaaga aaaaagcagt catcgatttt aagtccaatg ggcacattta tgacaatcgg 4501 atagttctga atggcatcga cctcaaagca tttcttgata gtctaccaga tgtgaaaatt 4561 gtcaagatga agtgtcctga tggaggagac aatgcagata gcagtaacac agctcttaat 4621 atgcctgtta ttcctatgaa tactattgca gaagcagtta ttgaaatgat taaccgagga 4681 cagattcaaa taacaattaa tggattcagt attagcaatg gactggcaac tactcagatc 4741 aacaataagg ctgcaactgg agaggaggtt ccccgtacca ttattgtaac cacccgttct 4801 cagtacgggt taccagaaga tgccatcgta tactgtaact ttaatcagtt gtataaaatt 4861 gacccttcta ctttgcagat gtgggcaaac attctgaagc gtgttcccaa tagtgtactc 4921 tggctgttgc gttttccagc agtaggagaa cctaatattc aacagtatgc acaaaacatg 4981 ggcctgcccc agaaccgtat cattttttca cctgttgctc ctaaagagga acacgtcagg 5041 agaggccagc tggctgatgt ctgcttggac actccactct gtaatgggca caccacaggg 5101 atggatgtcc tctgggcagg gacccccatg gtgactatgc caggagagac tcttgcttct 5161 cgagttgcag catcccagct cacttgctta ggttgtcttg agcttattgc taaaaacaga 5221 caagaatatg aagacatagc tgtgaagctg ggaactgatc tagaatacct gaagaaagtt 5281 cgtggcaaag tctggaagca aagaatatct agccctctgt tcaacaccaa acaatacaca 5341 atggaactag agcggctcta tctacagatg tgggagcatt atgcagctgg caacaaacct 5401 gaccacatga ttaagcctgt tgaagtcact gagtcagcag gatcctaccc atacgacgta 5461 cctgattatg caacttatcc ttatgatgta cctgattatg ctagctatcc ttatgatgtc 5521 ccagactacg catagcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca 5581 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt 5641 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt 5701 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt 5761 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca 5821 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct 5881 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg 5941 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg 6001 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt 6061 tacagatcca agctgtgacc ggcgcctacg ctagatgacc gagtacaagc ccacggtgcg 6121 cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga 6181 ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct 6241 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga 6301 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc 6361 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat 6421 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg 6481 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga 6541 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc 6601 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg 6661 cacctggtgc atgacccgca agcccggtgc ctgagtcgac aatcaacctc tggattacaa 6721 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata 6781 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc 6841 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg 6901 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac 6961 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat 7021 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 7081 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat 7141 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc 7201 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag 7261 tcggatctcc ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg 7321 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact 7381 cccaacgaaa ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct 7441 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc 7501 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc 7561 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta 7621 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg 7681 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 7741 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc 7801 tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 7861 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta 7921 gtgaggaggc ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg 7981 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 8041 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 8101 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 8161 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 8221 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 8281 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 8341 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 8401 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 8461 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 8521 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 8581 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 8641 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 8701 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 8761 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 8821 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 8881 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 8941 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 9001 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 9061 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 9121 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 9181 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 9241 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 9301 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 9361 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 9421 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 9481 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 9541 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 9601 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 9661 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 9721 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 9781 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 9841 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 9901 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 9961 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 10021 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 10081 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 10141 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 10201 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 10261 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 10321 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt 10381 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 10441 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 10501 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 10561 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg //