LOCUS       V033353                10568 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033353
VERSION     V033353
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5400
                     /label="Nlrp3(NM_145827)"
                     /note="Nlrp3(NM_145827)"
                     /gene="Nlrp3"
     CDS             5407..5433
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5437..5463
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5467..5493
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5538..5749
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5762..6030
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6057..6656
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6663..7251
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7325..7558
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7630..7751
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7770..7905
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7943..7959)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7967..7983
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7991..8021)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8036..8057
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8345..8933)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9104..9964)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9965..10069)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10543..10559
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgacgagt gtccgttgca agctggctca gtatctagag
     2341 gaccttgaag atgtggacct caagaaattc aaaatgcatt tggaagatta cccgcccgag
     2401 aaaggctgta tcccagtccc caggggccag atggagaagg cagatcactt ggatctagcc
     2461 acactcatga ttgacttcaa tggcgaggag aaggcctggg ccatggctgt gtggatcttt
     2521 gctgcgatca acaggcgaga cctctgggaa aaagctaaga aggaccagcc agagtggaat
     2581 gacacgtgta catcacattc ctctatggta tgccaggagg acagccttga agaagagtgg
     2641 atgggtttgc tgggatatct ctcccgcatc tccatttgta aaaagaagaa agattactgt
     2701 aagatgtaca gacgacatgt gagaagcagg ttctactcta tcaaggacag gaacgcgcgt
     2761 ctaggtgaga gtgtggacct caacagtcgc tacacgcagc tccaactggt caaggagcat
     2821 ccaagcaagc aggagcggga gcatgaactc ctgaccatcg gccggactaa aatgcgggac
     2881 agccccatga gttcccttaa gctggagctg ctgtttgagc ccgaggacgg gcactcggag
     2941 cctgtgcaca cagtggtgtt ccagggagca gcaggcatcg ggaaaaccat cctagccagg
     3001 aagattatgt tggactgggc actgggaaag ctcttcaaag acaaatttga ctatttgttc
     3061 tttatccact gccgagaggt gagcctcagg acgccaagga gtctagcaga cctgattgtc
     3121 agctgctggc ctgacccaaa cccaccagtg tgcaagatcc tgcgcaagcc ttccaggatc
     3181 ctcttcctca tggatggctt tgatgagcta caaggggcct ttgacgagca cattggggag
     3241 gtctgcacag actggcaaaa ggctgtgcgg ggagacattc tgctaagcag cctcatccga
     3301 aagaaactgc tgcccaaggc ctctctgctc ataacgacga ggccggtagc cttggagaaa
     3361 ctgcagcatc tcctggacca cccccgccat gtggagatcc taggtttctc tgaggccaaa
     3421 aggaaggagt atttctttaa gtatttctcc aacgagctgc aggcccggga ggccttcagg
     3481 ctgatccaag agaatgaggt cctctttacc atgtgcttca tccccctggt ctgctggatt
     3541 gtgtgcacgg ggctaaagca acagatggag accgggaaga gcctggccca gacctccaag
     3601 accactacgg ccgtctacgt cttcttcctt tccagcctgc tgcaatcccg ggggggcatt
     3661 gaggagcatc tcttctctga ctacctacag gggctctgtt cactggctgc ggatggaatt
     3721 tggaaccaga aaatcctatt tgaggagtgt gatctgcgga agcacggcct gcagaagact
     3781 gacgtctccg ctttcctgag gatgaacgtg ttccagaagg aagtggactg cgagagattc
     3841 tacagcttca gccacatgac tttccaggag ttcttcgctg ctatgtacta tttgctggaa
     3901 gaggaggcag agggggagac cgtgaggaaa ggaccaggag gttgttcaga tcttctgaac
     3961 cgagacgtga aggtcctact agaaaattac ggcaagtttg aaaaaggcta tctgattttt
     4021 gttgtccgat tcctctttgg ccttgtaaac caggagagaa cctcttattt ggagaagaaa
     4081 ctaagttgca agatctctca gcaagtcaga ctggaactac tgaagtggat tgaagtgaaa
     4141 gccaaggcca agaagctgca gtggcagccc agccaactgg aactgttcta ctgcctgtac
     4201 gagatgcagg aggaagactt tgtgcagagt gccatggacc actttcccaa aattgagatc
     4261 aacctctcta ccagaatgga ccacgtggtt tcctcctttt gtattaagaa ctgtcatagg
     4321 gtcaaaacgc tttccctggg tttttttcac aactcgccca aggaggaaga agaagagagg
     4381 agaggaggtc gacccttgga ccaggttcag tgtgttttcc cagacactca tgttgcctgt
     4441 tcttccagac tggtgaactg ctgcctcact tctagcttct gccgtggtct cttctcaagt
     4501 ctaagcacca accggagcct cactgaactg gacctcagtg acaatactct gggagacccg
     4561 ggcatgaggg tgctgtgtga ggcactccag cacccaggct gtaacattca gagactgtgg
     4621 ttggggcgct gcggactgtc ccatcaatgc tgcttcgaca tctcctctgt cctgagcagc
     4681 agccagaagc tggtggagct ggacctcagt gacaatgccc tgggggactt tggaatcaga
     4741 ttgctgtgtg tgggactgaa gcacctgctc tgcaacctcc agaaactgtg gttggtgagc
     4801 tgctgtctca catccgcgtg ttgtcaggat ctcgcattgg ttctgagctc caaccattct
     4861 ctgaccagac tgtacattgg agaaaatgcc ttgggagact caggagtcca agttttgtgt
     4921 gaaaagatga aggacccaca gtgtaacttg cagaagctgg ggttggtgaa ttccggcctt
     4981 acttcaatct gttgttcagc tctgacctct gtgctcaaaa ccaaccagaa cttcacacac
     5041 ctctatctac gaagcaatgc ccttggagac acaggactca ggctcctctg tgaggggctt
     5101 ctgcacccgg actgtaaact acagatgctg gaattagaca actgcagcct cacctcacac
     5161 agctgctgga atctctccac aattctgacc cacaaccaca gccttcggaa gctgaacctg
     5221 ggcaacaatg atcttggcga tctgtgcgtg gtgaccctct gtgaggtgct gaaacagcag
     5281 ggctgcctcc tgcagagcct acagttgggt gaaatgtact taaatcgtga aacaaaacgt
     5341 gccttagaag cgctccagga agaaaagcct gagctgacta tagtcttcga gatttcctgg
     5401 ggatcctacc catacgacgt acctgattat gcaacttatc cttatgatgt acctgattat
     5461 gctagctatc cttatgatgt cccagactac gcataggcgg ccgcgaagga tctgcgatcg
     5521 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     5581 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     5641 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     5701 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
     5761 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
     5821 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
     5881 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
     5941 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
     6001 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagatga
     6061 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca
     6121 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc
     6181 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     6241 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     6301 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     6361 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     6421 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     6481 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     6541 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     6601 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgagtcg
     6661 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     6721 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     6781 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     6841 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     6901 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     6961 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     7021 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     7081 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     7141 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     7201 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggtacct
     7261 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     7321 ggactggaag ggctaattca ctcccaacga aaataagatc tgctttttgc ttgtactggg
     7381 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7441 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7501 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     7561 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     7621 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     7681 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     7741 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc agttccgccc
     7801 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     7861 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac ttttgcagag
     7921 acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     7981 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     8041 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     8101 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     8161 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     8221 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     8281 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     8341 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     8401 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     8461 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     8521 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     8581 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8641 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8701 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     8761 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     8821 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     8881 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     8941 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     9001 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     9061 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     9121 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     9181 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     9241 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     9301 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     9361 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     9421 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9481 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9541 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9601 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9661 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9721 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     9781 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     9841 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     9901 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     9961 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    10021 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    10081 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
    10141 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
    10201 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
    10261 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
    10321 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
    10381 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
    10441 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
    10501 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
    10561 ccaagctg
//
