LOCUS       V043457                10160 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043457
VERSION     V043457
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5079
                     /label="Niban1(NM_022018)"
                     /note="Niban1(NM_022018)"
                     /gene="Niban1"
     promoter        5130..5341
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5354..5622
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5649..6248
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6255..6843
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6917..7150
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7222..7343
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7362..7497
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7535..7551)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7559..7575
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7583..7613)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7628..7649
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7937..8525)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8696..9556)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9557..9661)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10135..10151
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgggcggc tcagcttcca gccagctgga cgagggcaag
     2341 tgcgcctaca tccgagggaa aactgaggct tctatcaaaa acttcagccc ctactacagc
     2401 cggcagtatt ctgtagcttt ctgcaatcat gtgcgcagcg aggtggagca gcaaagagat
     2461 ttaacgtcac aatttctgaa gaccaagccc ccactggaac caggaacggt tttgtatgaa
     2521 gctgagctct cccagtttgc tgaagacatc aggaaatgga aagacagata cattgtgatt
     2581 aaaaacgatt ttgctgtgga aagctatgag agcaaagagg cctatcagag aggagctgtt
     2641 cctaaaagca ggattcttcc agccggtggc aaggtgttaa cctcagaaga ggaatatagt
     2701 ctcttatctg ataagcattt cccagacccc actgcttcca gcgagaagaa ctctcagccc
     2761 tttgtgctcc tccctaaggc attcccagtg tacctgtggc agccttacct caggcatggc
     2821 tacttctgtt tccatgaggc cgccgaacag cagaagttca gcgctcttct caatgactgc
     2881 atcagacacc tgaaccacga ttacatgaag cagacaacat ttgaagccca agccttttta
     2941 gaagctgtgc agttcttccg gcaggagaag ggtcactacg gctcatggga aatgaccacc
     3001 ggggatgaag tccaggtcct gagtaagctg gtgatggagg agctcctgcc gaccctccag
     3061 actgacctgc tgcctaaact gaaggggaag aagaatgaca gaaagagggc ctggtttgga
     3121 ctcctggagg aagcctacaa tctggttcag caccaagttt cagaaggatt aaatgccttg
     3181 aaggaggagt gcagagctct gacaaaggac ctggaaggga ccatccgctc agacatggat
     3241 cagattgtga cctcaaagaa ctttctaact gggaagatcc gagcaatggt ggctcagcca
     3301 gccgagcagt gctgtgggga gagcgtgcag cccttcctgg cgtccattct ggaggagcta
     3361 atggggccag tgagctctgg cttcagtgaa gtccgtgcac ttttcgaaaa agaagtagat
     3421 gaactcagtc agagcttcca tgctacccag gacagtgccc agctgaagga gggcctacag
     3481 cagctaatga agcttcctct ggattctgtg aagatggagc cttgctatac taaagtcacc
     3541 ctgcttccag agcgcctgct ggacctccag agccgcttca ggttccctca cgttgacctg
     3601 gtggtccaga ggactcagaa ctacatgcaa gagctcatgg agaatgctgt gttcacgttt
     3661 gagcagttgc tctccccata tctccaagga gaggcttcca gaataccagt ggccatcgag
     3721 aaggttaagc tccgtgtctt aaagcaatat gattacgaca gcagcaccat ccggaagaag
     3781 atcttccagg aggcattgat tcaaatcaca ctgcccactg tgcagaaggc gctggcatcc
     3841 acatgcaaac cagagcttca gaaatatgag cagttcatct ttgcagatca taccaacatg
     3901 atccacgttg agaacgtcta tgaagagatt ttgtatgaga tcctcctcga tgagaccctg
     3961 aaagtgataa cggaggcagc catcttgaag aaacacaact tatttgaaga caacatggcc
     4021 ttgcccagtg aaagtgtgtc cagcctgaca gacctcaaaa ccgccatggg gtcaaaccag
     4081 gccagcccag ccagaagagt atctgccatt ctcccaggag ctccagataa tgagctccca
     4141 agtaatgaag tgttccagga gccagaggaa aagaaggagc agcctggggt gcctggctca
     4201 ctggccatct ctgcgtccag ctgtccttca ggtggggatg ggcaggtgtc tgtggatcac
     4261 tctgctgggg gccctctcac tgtagagaat acagcaggac ccctcagctc acacttgtca
     4321 gaggtggaag ctggggggac ccttaaagat gaggaaccaa cctgccaaag tccagagcct
     4381 agcgctgtgc cagggtccct gaaggaactg aaaaagttgt tgactgtgac ggtgtccgta
     4441 gagtctgccc cggtggttga aaatgatatc cacaacggga cacctgttcc ccaagagaat
     4501 ataaaagaag aagaaagcaa gatccaccca gaagccagcc acccagctgc catccagcag
     4561 gacagctgtg aagaaaggga agtcagagag aaggaggccc agcctttgga agctgaggct
     4621 cctggggtgg acttggggat actgccagag ggtagaggct ctacctctca gtccaccagt
     4681 ggggggctca ctgagaacac cagctgtcca ggccccatag aggagccatt tgaggctcaa
     4741 gagccagcag agaaggtgct cccagccata gtttctacag aggacagccc ccaggcagga
     4801 ggtgaggctg agcattcggt gacagtcaca cctcaagaag atgccacgtt aagctctaac
     4861 cccatctgtc ccatggagag caatgaggtg gcccaggctt ctggggatca agaagtgctg
     4921 ggaggggagg atagctcagc ccttggtatg gatacagagc aagtcaatga tactcatgag
     4981 catgcttgtc agtggctggt ggaggatacc ctgagcactg acatcctagc cgtacatgat
     5041 tttgatgtga gctccccaga gcagccctca gaggagtggt agggatccgc ggccgcgaag
     5101 gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     5161 gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa
     5221 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     5281 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     5341 gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg
     5401 ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc
     5461 gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc
     5521 ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact
     5581 ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc
     5641 tacgctagat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc
     5701 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     5761 acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     5821 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     5881 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     5941 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     6001 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     6061 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     6121 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     6181 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     6241 gtgcctgagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     6301 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     6361 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6421 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6481 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6541 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6601 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     6661 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     6721 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     6781 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     6841 cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     6901 aaagaaaagg ggggactgga agggctaatt cactcccaac gaaaataaga tctgcttttt
     6961 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7021 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7081 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7141 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     7201 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     7261 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     7321 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     7381 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     7441 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     7501 acttttgcag agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt
     7561 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7621 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7681 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7741 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7801 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7861 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7921 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7981 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8041 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8101 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8161 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8221 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8281 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8341 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     8401 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8461 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8521 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8581 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8641 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8701 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8761 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8821 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     8881 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8941 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     9001 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9061 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9121 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9181 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9241 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9301 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9361 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9421 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9481 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9541 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9601 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9661 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     9721 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     9781 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     9841 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     9901 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     9961 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
    10021 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
    10081 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
    10141 cgacggccag tgccaagctg
//
