LOCUS       V039041                11203 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039041
VERSION     V039041
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5229
                     /label="Nfkb1(NM_008689)"
                     /note="Nfkb1(NM_008689)"
                     /gene="Nfkb1"
     CDS             5236..5301
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5345..5556
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5569..5837
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5866..5875
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5896..6558
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6628..6681
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6682..7281
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7282..7870
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7944..8177
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8249..8370
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8410..8545
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8578..8594)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8602..8618
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8626..8656)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8671..8692
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8980..9568)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9739..10599)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10600..10704)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11178..11194
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cagacgatga tccctacgga
     2341 actgggcaaa tgtttcattt gaacactgct ttgactcact caatatttaa tgcagaatta
     2401 tattcaccag aaataccact gtcaacagat ggcccatacc ttcaaatatt agagcaacca
     2461 aaacagaggg gatttcgatt ccgctatgtg tgtgaaggcc catcacacgg agggcttccg
     2521 ggagcctcta gtgagaagaa caagaaatcc tacccacagg tcaaaatttg caactatgtg
     2581 gggcctgcaa aggttatcgt tcagttggtc acaaatggaa aaaacatcca cctgcacgcc
     2641 cacagcctgg tgggcaagca ctgtgaggac ggggtatgca ccgtaacagc aggacccaag
     2701 gacatggtgg ttggctttgc aaacctggga atacttcatg tgactaagaa aaaggtattt
     2761 gaaacactgg aagcacggat gacagaggcg tgtattaggg gctataatcc tggacttctg
     2821 gtgcattctg accttgccta tctacaagca gaaggcggag gagaccggca actcacagac
     2881 agagagaagg agatcatccg ccaggcagcc gtgcagcaga ccaaggagat ggacctgagc
     2941 gtggtgcgcc tcatgttcac agccttcctc cctgacagca ctggcagctt cactcggaga
     3001 ctggagcctg tggtgtcaga cgccatctat gatagcaaag ccccgaatgc atccaacctg
     3061 aaaatcgtga gaatggacag aacagcagga tgtgtgacgg gaggggagga gatttacctt
     3121 ctctgtgaca aggttcagaa agatgacatc cagattcggt tttatgaaga ggaagaaaat
     3181 ggcggagttt gggaaggatt tggggacttt tcccccacag atgttcatag acagtttgcc
     3241 attgtcttca aaacgccaaa gtataaggat gtcaacatta caaagccagc ttccgtgttt
     3301 gttcagcttc ggaggaaatc agacctggaa actagtgaac cgaaaccctt tctctactac
     3361 cctgaaatca aagacaaaga ggaagtgcaa aggaaacgcc agaagcttat gccgaacttc
     3421 tcggacagct tcggtggcgg cagtggagcg ggagccggtg gtggaggcat gttcggtagt
     3481 ggcggtggcg gagggagtac cggaagccct ggcccagggt atggctactc gaactacgga
     3541 tttcctccct acggtgggat tacattccat cccggagtca cgaaatccaa cgcaggggtc
     3601 acccatggca ccataaacac caaatttaaa aatggcccta aagattgtgc caagagtgat
     3661 gacgaggaga gtctgactct ccctgagaag gaaactgaag gtgaagggcc cagcctgccc
     3721 atggcctgca ccaagacgga acccatcgcc ttggcatcca ccatggaaga caaggagcag
     3781 gacatgggat ttcaggataa cctctttctt gagaaggctc tgcagctcgc caggcgacac
     3841 gccaacgccc ttttcgacta cgcagtgacg ggggatgtga agatgttgct ggccgtgcaa
     3901 cgccatctca ccgccgtgca ggatgagaat ggggacagtg tcttacactt agccatcatc
     3961 cacctccacg ctcagcttgt gagggatctg ctggaagtca catctggttt gatctctgat
     4021 gacatcatca acatgagaaa tgacctgtat cagacacctc tgcacttggc cgtgatcacc
     4081 aagcaggaag atgtagtaga ggatttgctg agggttgggg ctgacctgag ccttctggac
     4141 cgctggggca actctgtcct gcacctagct gccaaagaag gacacgacag aatcctcagc
     4201 atcctgctca agagcagaaa agcagcgccc cttatcgacc accccaatgg ggaaggtcta
     4261 aatgccatcc acatagctgt gatgagcaat agcctgccat gtctgctgct gctggtggct
     4321 gccggggcag aagtcaatgc tcaggagcag aagtctgggc gcacagcgct gcacctggcc
     4381 gtggagtacg acaacatctc cttggctggc tgcctgcttc tggagggtga tgcccacgtg
     4441 gacagtacca cctatgatgg gactacacct ctgcatatag cggccggaag agggtccacc
     4501 agactggcag ctcttctcaa agcagcagga gcagaccccc tggtggagaa ctttgagcct
     4561 ctctatgacc tggacgactc ttgggagaag gctggagaag atgagggagt ggtgccaggt
     4621 accacacccc tggacatggc tgccaactgg caggtatttg acatactaaa tgggaaaccg
     4681 tatgagcctg tgttcacatc tgatgatata ctaccacaag gggacatgaa gcagctgaca
     4741 gaagacacga ggctacaact ctgcaaactg ctggaaattc ctgatccaga caaaaactgg
     4801 gccactctgg cacagaagtt gggtctgggg atactgaaca atgccttccg gctgagtcct
     4861 gctccttcta aaactctcat ggacaactat gaggtctctg ggggtaccat caaagagctg
     4921 atggaggccc tgcaacagat gggctacaca gaggccattg aagtgatcca ggcagccttc
     4981 cgcaccccgg caaccacagc ctccagcccc gtgaccactg ctcaggtcca ctgtctgcct
     5041 ctctcgtctt cctccacgag gcagcacata gatgaactcc gggatagtga cagcgtctgt
     5101 gacagtggtg tggagacatc cttccgcaaa ctcagcttta cagagtctct tactggagac
     5161 agcccactgc tatctctgaa caaaatgccc cacggttatg ggcaggaagg acctattgaa
     5221 ggcaaaattg gatccgacta caaagaccat gacggtgatt ataaagatca tgacatcgac
     5281 tacaaggatg acgatgacaa gtagcggccg cgaaggatct gcgatcgctc cggtgcccgt
     5341 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     5401 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     5461 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     5521 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
     5581 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
     5641 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
     5701 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
     5761 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
     5821 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggagagc
     5881 gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc
     5941 gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac
     6001 aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg
     6061 ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac
     6121 gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg
     6181 ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg
     6241 gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac
     6301 gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc
     6361 accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc
     6421 aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc
     6481 gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag
     6541 acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc
     6601 gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac
     6661 gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc
     6721 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     6781 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     6841 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     6901 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     6961 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     7021 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     7081 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     7141 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     7201 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     7261 acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga aagattgact
     7321 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     7381 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     7441 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7501 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     7561 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     7621 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     7681 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     7741 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     7801 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     7861 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     7921 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gataagatct
     7981 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     8041 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     8101 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     8161 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     8221 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     8281 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     8341 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     8401 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8461 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     8521 tagtgaggag gcttttttgg aggcctagac ttttgcagag accaaattcg taatcatgtc
     8581 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     8641 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     8701 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     8761 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     8821 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8881 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8941 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     9001 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     9061 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     9121 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     9181 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     9241 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9301 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9361 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9421 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9481 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9541 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9601 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9661 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9721 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9781 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9841 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9901 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9961 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    10021 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    10081 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    10141 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    10201 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    10261 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    10321 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    10381 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    10441 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10501 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10561 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10621 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10681 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
    10741 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
    10801 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
    10861 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
    10921 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
    10981 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
    11041 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
    11101 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
    11161 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
