LOCUS V037193 11107 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V037193 VERSION V037193 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2317..5133 /label="Nfatc1(NM_001415721)" /note="Nfatc1(NM_001415721)" /gene="Nfatc1" CDS 5140..5205 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 5249..5460 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 5473..5741 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 5770..5779 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 5800..6462 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 6532..6585 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 6586..7185 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 7186..7774 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7848..8081 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 8153..8274 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 8314..8449 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(8482..8498) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 8506..8522 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(8530..8560) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8575..8596 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8884..9472) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9643..10503) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(10504..10608) /gene="bla" /label="AmpR promoter" primer_bind 11082..11098 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc caagtaccag ctttccagtc 2341 ccttccaagt ttccactcgg ccctccggcc gcagtctgcg ggagcggaga aactttgcgg 2401 cccgcgccgc tcgccggcgg caccatgaag gcggccgagg aagaacacta cagttatgcg 2461 tcccctagcg tcacctcaac tctgcccctt cccacagcac actccgcctt gccagcggca 2521 tgccatgacc tccagacgtc caccccgggc atctcagctg ttccttcggc caatcacccc 2581 tctggttatg ggggcgctgt ggacagtggg ccttcaggat acttcctgtc ctccggcaac 2641 acgagaccca atggggcccc gactctcgag agtcctcgaa tcgagatcac ctcctatctg 2701 ggccttcacc acggcagcaa ccaatttttc cacgatgtgg aggtggaaga cgtacttcct 2761 agctgcaagc ggtcaccatc tacagcaacc ctgcacctgc ccagtctgga agcctacaga 2821 gacccctcct gcctgagccc agccagcagc ctgtcctcca ggagctgtaa ctctgaggcg 2881 tcctcctatg agtccaacta ttcatacccg tacgcatccc cccagacctc tccatggcag 2941 tcaccctgtg tgtctcccaa gaccacggac ccagaggagg gctttccccg aagcctgggt 3001 gcctgccact tgctgggatc gccaaggcac tccccatcca cctctcctcg tgcaagcgtc 3061 accgaggaga gctggctcgg tgcccgtggc tcccggccca catccccctg caacaagcgc 3121 aaatacagtc tcaacggcag gcagccctcc tgctcacccc accactcgcc cacaccatcc 3181 ccccatggct cccctcgggt cagcgtgacg gaagacacct ggcttggtaa caccacccag 3241 tacaccagct ctgctattgt ggcagccatc aacgccctga ccactgatag cactctggac 3301 ctgggtgacg gtgtccctat caagtctcga aagacagccc tggagcacgt gccctctgtg 3361 gccctcaagg tagagccggc tggggaagac ctgggcacca caccacccac ttctgacttc 3421 ccacccgagg agttcgcctt ccagcacctt cggaagggtg ccttttgtga gcagtatctg 3481 tcggtgccac aggcctcgta tcagtgggca aagcccaagt ctctatcccc aacgtcatac 3541 atgagcccat ccctgcctgc ccttgactgg cagctgccgt cgcactctgg tccgtatgaa 3601 ctccggatcg aggtgcaacc caagtctcac cacagggctc actatgagac ggaaggcagc 3661 cggggagctg tgaaggcttc tgctggagga caccctgtcg tgcagctaca tggttacttg 3721 gaaaacgagc ctctcacgct ccagctgttc attgggacgg ctgacgaccg cctgctacgg 3781 ccccatgcct tctaccaggt ccaccggatc acggggaaga ctgtctccac caccagccac 3841 gaggctatcc tgtccaacac caaagtcctg gagatcccac tgcttccaga aaataacatg 3901 cgagccatca tcgactgtgc tgggatcctg aagctcagaa actctgatat cgagctgagg 3961 aaaggggaga cagacatcgg gagaaagaac accagggtgc ggctggtctt ccgagtccac 4021 atcccccagc ccagcggccg gacgctgtct ctccaggtgg cctcaaaccc gatcgagtgc 4081 tcccagcggt cggcccagga actgcccctc gtggagaagc agagcacaga cagctacccg 4141 gtcattggag ggaagaagat ggtgttgtct ggccataact ttctgcaaga ctccaaggtc 4201 atcttcgtgg agaaggcccc agatggccac catgtctggg agatggaagc gaaaactgac 4261 cgagacctgt gcaagccaaa ttccctggtg ctggagatac cacctttccg caaccagaga 4321 ataaccagcc ccgtccaagt cagcttctat gtctgcaacg ggaagcggaa gagaagccag 4381 taccagcatt tcacgtacct tcctgccaat gttccaatta taaagacaga acccacggac 4441 gactttgagc cagctctgac ctgtggacca atgagccagg gacttagccc tctgccgagg 4501 ccttactata gccaacaact caccgcgcct cctgaccctg gctcctgcct tgtggccggc 4561 tttgccccct gctcacagag gaacacgctg atgcccacgt ctcccaatgc aagcccaaag 4621 ctccacgacc tttcctctcc cgcctacgcc aagggcctcg ccaacccagg ccacagtggt 4681 caccttggac ttcagccacc agcttcagag gcccccacca tgcaggaatt gccgagaccc 4741 atggccgtcc agcccaactc gcctgagcag cccacatccg tcaggctaca gccgcaggtg 4801 agtccacatc tgaacagtag ctgtcccctt ggtctccaac aagtgctcta tcccaacagc 4861 ccctcctctc cacttccacc tgctgcccac gagccaacct gcttacagcc ctcagctctc 4921 cctcctgatg tgggccaccg gcagcaacaa ccgcagaagg ttcacagaaa tgaatctccg 4981 tccatactgc cagaggtgtg tgaggacagt gaccataact tggcccctgt tcctgtagtg 5041 atcaagcaag agcctgagga attggaccag ttgtacctgg atgatgtaaa tgagatcatt 5101 cgtaacgacc tctccagcac aatcccccac tccggatccg actacaaaga ccatgacggt 5161 gattataaag atcatgacat cgactacaag gatgacgatg acaagtagcg gccgcgaagg 5221 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag 5281 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac 5341 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat 5401 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag 5461 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc 5521 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg 5581 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct 5641 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc 5701 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct 5761 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc 5821 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc 5881 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc 5941 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc 6001 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc 6061 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc 6121 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc 6181 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac 6241 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc 6301 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg 6361 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc 6421 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg 6481 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga 6541 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag 6601 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc 6661 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg 6721 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg 6781 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg 6841 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg 6901 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg 6961 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc 7021 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc 7081 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc 7141 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat 7201 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 7261 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 7321 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 7381 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 7441 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 7501 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 7561 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc 7621 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 7681 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag 7741 acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta 7801 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat 7861 tcactcccaa cgaagataag atctgctttt tgcttgtact gggtctctct ggttagacca 7921 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 7981 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 8041 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 8101 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt 8161 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 8221 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 8281 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 8341 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 8401 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc 8461 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac 8521 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 8581 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 8641 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 8701 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 8761 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 8821 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 8881 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 8941 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 9001 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 9061 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 9121 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 9181 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 9241 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 9301 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 9361 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 9421 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 9481 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 9541 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 9601 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 9661 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 9721 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 9781 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 9841 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 9901 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 9961 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 10021 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 10081 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 10141 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 10201 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 10261 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 10321 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 10381 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 10441 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 10501 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 10561 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 10621 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 10681 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 10741 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 10801 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 10861 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 10921 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 10981 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 11041 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc 11101 caagctg //