LOCUS       V025023                10367 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025023
VERSION     V025023
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4410
                     /label="Nek8(NM_080849)"
                     /note="Nek8(NM_080849)"
                     /gene="Nek8"
     CDS             4417..4482
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4526..4737
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4750..5018
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5047..5056
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5077..5739
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5809..5862
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5863..6462
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6463..7050
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7124..7357
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7429..7550
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7569..7704
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7742..7758)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7766..7782
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7790..7820)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7835..7856
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8144..8732)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8903..9763)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9764..9868)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10342..10358
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg agaagtacga gcggatccga
     2341 gtggtgggaa gaggtgcctt cgggattgtg cacctgtgcc tgcgaaaggc cgaccagaag
     2401 ctggtgatcc tcaagcagat cccagtggaa cagatgacca aagaagagag gcaggcggcc
     2461 cagaacgagt gccaggtgct caagctgctc aaccacccca acgtcatcga gtactatgag
     2521 aatttcctag aggacaaggc cctcatgatt gctatggagt acgcaccagg tggcaccttg
     2581 gccgagttca tccagaagcg ctgcaactct ctgctagagg aggagacgat ccttcacttc
     2641 tttgtgcaga tcctgctggc tctgcatcac gtgcacacac atctcatcct gcatcgggac
     2701 ctcaagacac agaacatcct tcttgacaaa caccgcatgg tggtcaagat cggtgacttt
     2761 ggcatctcta agatcctcag cagcaagagc aaggcctaca cggtggtggg tacaccctgc
     2821 tacatctccc ctgagctgtg tgagggcaaa ccctacaacc agaagagtga catctgggct
     2881 ctgggctgtg tcttatatga gctggccagc ctcaagaggg ccttcgaggc tgcaaacttg
     2941 ccagccctgg tgctgaagat catgagtggc accttcgcac ccatctctga ccggtacagc
     3001 cctgagcttc gccagctcgt cctgagtctg cttagcctgg aacctgcaca gaggccaccc
     3061 ctcagccaca tcatggcgca gcccctctgc atccgggccc tactcaacat ccacaccgat
     3121 gtgggcagcg tgcgcatgcg gagggcagag aagtccctga ccccaggacc acctatagcc
     3181 tctggcagca cagggagcag ggccaccagt gcccgatgta ggggtgtccc ccggggacct
     3241 gtgagaccgg ctatcccacc gccactgtct tcggtctacg cttggggtgg tggccttagc
     3301 agtcccctga ggttgccaat gctcaacacg gaggtggtcc aggtagccgc tgggcgcacg
     3361 cagaaggctg gcgttacgcg ctctggacgc ctcatcctgt gggaggcccc acccctggga
     3421 gctggaggcg gcacactcct tcctggagcc gtggagctgc ctcagcccca gtttgtctct
     3481 cgtttcctgg aaggccagtc aggtgtgacc atcaagcatg tggcctgcgg ggacctgttc
     3541 accgcttgct tgacagaccg aggcattatc atgacgttcg gcagtggcag caatgggtgt
     3601 ttaggtcacg gcaacctgac tgacatcagc cagcccacca ttgtagaagc cttgctgggc
     3661 tatgagatgg tgcaggtggc ctgtggggcc tctcatgtgc tggccctgtc cacagatgga
     3721 gagttattcg cctggggcag aggagatggt ggtagactgg gactaggcac cagggagtcc
     3781 cataactgcc ctcagcaggt gcctgtggcc ccaggacagg aagctcagag agttgtctgt
     3841 ggtattgact cttccatgat cctcacctct ccaggcaggg tcctggcctg tggaagtaac
     3901 aggttcaaca agctaggctt ggaccaccta tccctggacg aggagcctgt tccctaccag
     3961 caagtggagg aggccctgag cttcacacca ctgggctctg cacctctgga ccaggagccc
     4021 ctgctgtgtg tggaccttgg cactgctcat tcagctgcta tcactgcttc tggggactgc
     4081 tatacttttg gcagcaatca acatggacag ctgggcacca gttcccgcag ggtcagcagg
     4141 gcaccctgtc gggtccaagg cctggagggc atcaagatgg tgatggtggc ctgcggggat
     4201 gccttcactg tggccgttgg ggcagagggg gaagtgtatt cttggggcaa aggaacccga
     4261 ggccgactgg gaaggaggga tgaggatgcg gggctgccta ggccagtgca gctggatgag
     4321 actcatcctt acatggtgac ttcagtgtcc tgctgccatg ggaacactct gctggctgtt
     4381 cgatcagtca cagatgaacc agttcccccc ggatccgact acaaagacca tgacggtgat
     4441 tataaagatc atgacatcga ctacaaggat gacgatgaca agtagcggcc gcgaaggatc
     4501 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     4561 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg
     4621 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     4681 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg
     4741 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat
     4801 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc
     4861 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg
     4921 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac
     4981 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg
     5041 ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc
     5101 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc
     5161 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc
     5221 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc
     5281 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag
     5341 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc
     5401 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc
     5461 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg
     5521 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg
     5581 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc
     5641 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg
     5701 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc
     5761 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga
     5821 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc
     5881 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     5941 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     6001 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     6061 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     6121 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     6181 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     6241 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     6301 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     6361 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     6421 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac
     6481 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     6541 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     6601 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     6661 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     6721 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     6781 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     6841 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     6901 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     6961 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tccgccttcg ccctcagacg
     7021 agtcggatct ccctttggcc gcctccccgc ctggtacctt taagaccaat gacttacaag
     7081 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     7141 tcccaacgaa aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     7201 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7261 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7321 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     7381 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     7441 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7501 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     7561 ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     7621 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     7681 agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat
     7741 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     7801 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     7861 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     7921 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7981 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     8041 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8101 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8161 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8221 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8281 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8341 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8401 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8461 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8521 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8581 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8641 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8701 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8761 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8821 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8881 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8941 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     9001 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     9061 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9121 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     9181 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     9241 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     9301 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     9361 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9421 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     9481 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     9541 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9601 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9661 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9721 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9781 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9841 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     9901 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     9961 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
    10021 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
    10081 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
    10141 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
    10201 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
    10261 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
    10321 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
