LOCUS       V039887                11567 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039887
VERSION     V039887
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5661
                     /label="Ncam1(NM_001404722)"
                     /note="Ncam1(NM_001404722)"
                     /gene="Ncam1"
     promoter        5726..5937
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5950..6218
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6247..6256
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6277..6939
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7009..7062
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7063..7662
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7663..8250
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8324..8557
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8629..8750
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8769..8904
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8942..8958)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8966..8982
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8990..9020)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9035..9056
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9344..9932)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10103..10963)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10964..11068)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11542..11558
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc tgcgaactaa ggatctcatc
     2341 tggactttgt ttttcctggg aactgcagtt tccctgcagg tagatattgt tcccagccaa
     2401 ggagaaatca gcgttggaga gtccaaattc ttcctgtgtc aagtggcagg agatgccaaa
     2461 gataaggaca tctcctggtt ctcccccaat ggggagaagc tgagcccaaa ccagcagcgg
     2521 atctcagtgg tgtggaatga tgacgactcc tctaccctca ccatctacaa cgccaacatc
     2581 gacgatgccg gcatatacaa gtgtgtggtc acggctgagg acggcaccca gtctgaggcc
     2641 actgtcaacg tgaagatctt ccagaagctc atgttcaaga atgcaccaac cccacaggag
     2701 tttaaggaag gggaggatgc tgtgattgtc tgtgatgtgg tcagctccct gcctccaacc
     2761 atcatctgga aacacaaagg ccgagatgtc attctgaaaa aagacgtccg gttcatagtc
     2821 ctgtccaaca actacctgca gatcaggggc atcaagaaaa cagatgaggg tacttaccgc
     2881 tgtgagggca ggatcctggc ccgcggggaa atcaacttca aggacattca ggtcattgtg
     2941 aatgtaccac ccactgtcca ggccagacag agcatcgtga atgccactgc caacctgggc
     3001 cagtctgtca ccctggtgtg tgatgccgat ggcttcccag agcccaccat gagctggaca
     3061 aaggatgggg aacccataga gaacgaggag gaagatgacg agaagcacat cttcagtgat
     3121 gacagctccg agctgaccat caggaatgtg gataaaaacg acgaggccga atacgtctgc
     3181 atcgcagaga acaaggctgg cgagcaggat gcctccatcc acctcaaggt ctttgcaaag
     3241 cccaaaatca cctatgtaga gaatcagacg gccatggaac tagaggagca agtcactctg
     3301 acctgtgaag cctccggaga ccccatcccc tccatcacct ggagaacatc cacccggaac
     3361 atcagcagtg aagaaaagac tctggatggg cacatggtgg ttcgcagcca tgctcgtgtg
     3421 tcctccttga ccctgaagag catccagtac acagatgctg gagaatacat ctgcactgcc
     3481 agcaacacca tcggccagga ttcccagtcc atgtacctcg aagttcaata tgctcccaag
     3541 ctccagggcc ctgtggctgt gtacacttgg gaagggaacc aggtgaacat cacctgtgag
     3601 gtcttcgcct acccaagtgc cacaatctcc tggttccgag atggtcagtt gctgccaagc
     3661 tccaactaca gcaatatcaa gatctacaac accccatctg ctagctatct ggaggtgacc
     3721 cctgattcag aaaatgactt tggaaactac aactgtacag cagtgaaccg tattggacag
     3781 gagtccttgg aattcatcct tgttcaagca gacacaccgt cttctccatc cattgaccgg
     3841 gtggaaccat actccagcac agcgcaggtg cagtttgatg agccagaggc cacagggggc
     3901 gtgcccattc tcaagtacaa ggctgagtgg aagtcgctgg gcgaagaatc ctggcatttc
     3961 aagtggtatg atgccaaaga agccaacatg gaaggcattg tcaccatcat gggcctgaaa
     4021 cctgagacga ggtactcggt acgactggcg gccctcaacg gcaaggggct gggcgagatc
     4081 agtgcagcca ctgagttcaa gacacagcca gtccgggaac ccagtgcacc caagctggaa
     4141 gggcagatgg gagaggacgg gaactccatc aaggtgaacc tgatcaagca ggatgacgga
     4201 ggctccccca tcagacacta tctggtcaag tacagagcgc tcgcctctga gtggaaaccg
     4261 gaaatcaggc tcccatccgg cagtgaccac gtcatgctca agtccctgga ctggaacgca
     4321 gagtatgaag tctatgtggt agctgaaaac cagcaaggaa aatccaaggc agctcacttt
     4381 gtgttcagga cctcagccca gcccacggcc atcccagcca atggcagccc caccgcaggc
     4441 ctgagcacag gcgccattgt gggcatcctc attgtcatct tcgtcctgct cctggtggtc
     4501 atggacatca cctgctactt cctgaacaag tgtggcctgc tcatgtgcat cgctgttaac
     4561 ctgtgtggca aagctgggcc cggagccaag ggcaaagaca tggaggaggg caaggctgct
     4621 ttctcgaaag atgagtcaaa agaacccatt gtggaggtcc gaacggagga agaacggact
     4681 ccaaaccatg atggggggaa gcacacagag cccaacgaga ccacgccgct gacagaaccc
     4741 gagctgcctg ccgacaccac agccactgtc gaggacatgc tgccttctgt caccaccgtc
     4801 accactaact ctgacactat caccgaaacc tttgccactg ctcagaacag ccctaccagt
     4861 gagaccacta cactgacctc cagtattgcc ccaccggcca caactgtgcc agactcaaat
     4921 tctgtgcccg ctggtcaggc caccccttcc aagggtgtca ctgcttcatc ctcgtcccca
     4981 gcctcagccc ccaaagttgc tcctctggtt gacctgagtg atactccaac ttcagctccc
     5041 tctgctagca atctgtcctc cactgtcttg gctaaccaag gagctgtact cagtcctagc
     5101 acccctgcca gtgcgggaga gacctccaag gcccctccag ccagtaaggc ctcccctgct
     5161 cccaccccca ctccagctgg ggcagccagc cccttagcag cagtagccgc ccctgccaca
     5221 gatgcccccc aggccaagca ggaagccccc agcaccaaag gtccggaccc agagcccacc
     5281 cagcctggca ccgtgaagaa cccacctgag gcagccacag cccctgctag cccgaagagc
     5341 aaggctgcaa ccacaaaccc ttcccagggc gaggacttaa aaatggacga agggaacttc
     5401 aagaccccag atattgacct tgcaaaggat gtttttgcag ccctgggctc tcctgctccc
     5461 gccactgggg ccagtggaca agcctctgag cttgctcctt ccactgcaga cagcgctgtg
     5521 cctcccgcac cagcaaagac cgaaaagggc cctgtagaaa caaagtctga gcccccggag
     5581 tcagaagcca agccagcgcc aactgaagtc aagacggtcc ccaacgatgc cacacaaaca
     5641 aaagagaatg agagcaaagc atagggatcc ctgcgatatc cagcgcggcc gcgaaggatc
     5701 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     5761 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg
     5821 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     5881 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg
     5941 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat
     6001 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc
     6061 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg
     6121 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac
     6181 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg
     6241 ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc
     6301 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc
     6361 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc
     6421 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc
     6481 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag
     6541 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc
     6601 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc
     6661 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg
     6721 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg
     6781 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc
     6841 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg
     6901 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc
     6961 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga
     7021 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc
     7081 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     7141 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     7201 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     7261 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     7321 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     7381 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     7441 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     7501 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     7561 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     7621 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac
     7681 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     7741 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     7801 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     7861 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     7921 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     7981 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     8041 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     8101 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     8161 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tccgccttcg ccctcagacg
     8221 agtcggatct ccctttggcc gcctccccgc ctggtacctt taagaccaat gacttacaag
     8281 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     8341 tcccaacgaa aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     8401 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     8461 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     8521 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     8581 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     8641 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     8701 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     8761 ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     8821 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     8881 agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat
     8941 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     9001 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     9061 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     9121 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     9181 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     9241 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     9301 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     9361 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     9421 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     9481 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     9541 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     9601 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     9661 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9721 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9781 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9841 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9901 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9961 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    10021 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    10081 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    10141 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    10201 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    10261 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    10321 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    10381 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    10441 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    10501 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    10561 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    10621 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    10681 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    10741 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    10801 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    10861 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    10921 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    10981 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    11041 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
    11101 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
    11161 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
    11221 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
    11281 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
    11341 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
    11401 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
    11461 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
    11521 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
