LOCUS       V037866                11314 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037866
VERSION     V037866
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..5374
                     /label="Nat10(NM_153126)"
                     /note="Nat10(NM_153126)"
                     /gene="Nat10"
     CDS             5381..5407
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5411..5437
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5441..5467
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5512..5723
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5736..6004
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6039..6746
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             6756..6809
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6810..7409
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7410..7997
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8071..8304
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8376..8497
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8516..8651
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8689..8705)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8713..8729
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8737..8767)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8782..8803
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9091..9679)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9850..10710)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10711..10815)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11289..11305
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgaatcg gaagaaggtg gataaccgaa ttcgaattct
     2341 cattgagaat ggcgtagctg agcggcagag gtctcttttt gttgtagttg gggatcgagg
     2401 aaaagatcag gtggttattc ttcatcatat gttgtccaag gcaactgtga aggctcggcc
     2461 ctcagtcctg tggtgttata agaaagagct gggatttagc agtcaccgga agaagaggat
     2521 gcggcagcta cagaagaaaa taaagagtgg gaccttgaac ttaaagcaag atgacccctt
     2581 tgagcttttt gtagcagcca caaacattcg ctactgctac tacaatgaaa cccacaagat
     2641 tctgggcaat actttcggca tgtgtgtcct ccaggatttt gaagcgttaa ctccgaactt
     2701 gttggccaga actgtagaaa cagtagaagg tggtggactg gtggtcatcc tcctgcggac
     2761 catgaactcg cttaagcagc tgtacacgat gactatggat gtgcattcca ggtacaggac
     2821 tgaggcccat caggacgtgg tgggaagatt taacgagagg tttattctct ctctggcctc
     2881 ttgtaagaag tgtctggtca ttgacgatca gctcgacatc ctgcccatct cctcccacgt
     2941 ggccagcatt gaagccttac ctcctcaggc cccggatgag aatctcagtc ctgctgctct
     3001 ggagctgctg gagttgaaag agagcttgca ggacactcag cccgtgggtg ttctggtgga
     3061 ctgctgcaag accctggacc aggccaaagc tgtcttgaaa ttcattgagg ggatctcgga
     3121 gaagactcta aggagtactg tagccctcac cgctgcccga ggaaggggca agtctgcggc
     3181 cctgggcctg gctatcgctg gagcagtggc attcgggtat tccaatattt ttgttacctc
     3241 cccaagcccg gataacctcc acacgctgtt tgaatttgta tttaaaggat ttgatgctct
     3301 gcagtatcag gagcatctgg attatgagat tgtacagtcg ctgaaccccg agtttaataa
     3361 agcggtgatc agggtcaatg tgttccgaga gcacagacag actattcagt acatccaccc
     3421 tgcagatgct gtgaaactgg gccaggctga gctggttgtg atagatgaag ctgccgctat
     3481 tcccctcccc ctggtgaaga gcctgcttgg gccctacctg gttttcatgg catctactat
     3541 caatggctac gagggcactg gccggtcact gtccctcaag ctcattcaac aactccgtca
     3601 gcagagtgcc cagagccagg tcagcaccac tgctgagaac aagaccacaa cgacagccag
     3661 actggcctca gctcgaacct tgcatgaggt ttccctccaa gagtcaatcc gatacgcccc
     3721 tggggatgca gtggagaagt ggcttaatga cctgctgtgc ctggattgcc tcaacatcac
     3781 ccgcatcgtt tccggctgcc ccttgcctga ggcctgtgag ctctactatg ttaacagaga
     3841 taccctcttt tgctaccaca aggcctctga agttttcctc cagcggctca tggctctcta
     3901 tgtggcttct cattacaaga actctcccaa cgacctgcag atgctctcag acgctcctgc
     3961 tcaccacctc ttctgcctcc tgccacctgt gccccccacc cagaatgccc tgcctgaagt
     4021 gcttgcagtt gtccaggtgt gccttgaggg agagatttct cgtcagtcca ttttgaatag
     4081 cctctctcga ggcaagaagg cttctgggga cctgattcca tggacagtgt cggaacagtt
     4141 tcaagatcca gactttggag gcctttctgg tggacgggtt gttcgaattg ctgttcaccc
     4201 agattatcaa gggatgggat atggcagccg agcgctgcag ctgctgcaga tgtattatga
     4261 aggcaagttc ccttgtctgg aggaaaaagt cttggagaca ccacaagaaa tccgcaccgt
     4321 gagcagtgag gctgtcagct tgttggaaga agttattact ccccggaagg atctgccccc
     4381 cttgctcctc aagttgaatg agaggcctgc agagcgcctg gattacctgg gggtttccta
     4441 tgggctgacc cccaggcttc tcaagttctg gaaacgagct ggatttgttc ctgtctattt
     4501 gagacagacc ccaaacgacc tgactgggga acactcgtgc attatgctga agacgctggc
     4561 cgatgaggat gaggctgagc agggagcttg gctggcagca ttttggaaag atttccgaag
     4621 acggttcctg gccttgctgt cataccagtt cagcaccttc tctcctgccc tgtctctgaa
     4681 catcattcag aacaggaacg tagccaagtc agccctgcca gccctaggcc gggagcatct
     4741 ggaggcactt ttccttccct atgacctgaa gcgtctagag atgtactctc ggaacatggt
     4801 cgattaccac ctcatcatgg atcttatccc agccatttcc cgcttgtact tcctgaacca
     4861 gctaggagac ctgtccctgt cggctgccca gtcggctctt ctcttgggaa ttggcctaca
     4921 gcacaagtct gtggatcagc tggaaaagga gattgagctg ccctcaggcc agttgatggg
     4981 gcttttcaac cggatcatcc gcaaagttgt gaagctgttc aatgatgtgc aggaaaaggc
     5041 tattgaggag cagatggtgg cagtgaagga tgtggtcatg gagcccacta tgaagaccct
     5101 gagtgatgac ctggatgaag cagcaaagga attccaggag aaacacaaga aggaagtcgg
     5161 gaagctgaag gacatggacc tctctcaata tgtaattcgt ggggacgatg aagagtggaa
     5221 tgaagttttg agcaaagcag ggcagaatgc ctccattgtt agtttgaaaa gtgacaagaa
     5281 aaggaaactg gaaacaaaac aagaacccaa acagagcaag aagttgaaga agagagataa
     5341 caacagaaag gatatgaaac tgaagcgaaa gaagggatcc tacccatacg acgtacctga
     5401 ttatgcaact tatccttatg atgtacctga ttatgctagc tatccttatg atgtcccaga
     5461 ctacgcatag gcggccgcga aggatctgcg atcgctccgg tgcccgtcag tgggcagagc
     5521 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct
     5581 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
     5641 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
     5701 acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg
     5761 cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg
     5821 cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac
     5881 cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt
     5941 gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca
     6001 gatccaagct gtgaccggcg cctacgctag acgccaccat ggtgagcaag ggcgaggagg
     6061 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
     6121 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     6181 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
     6241 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
     6301 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     6361 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
     6421 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     6481 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
     6541 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     6601 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
     6661 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     6721 ccggcggcat ggacgagctg tacaagggat ctcgcgaggg cagaggaagt cttctaacat
     6781 gcggtgacgt ggaggagaat cccggcccta tgaccgagta caagcccacg gtgcgcctcg
     6841 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     6901 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     6961 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     7021 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     7081 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     7141 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     7201 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     7261 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     7321 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     7381 ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa
     7441 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     7501 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     7561 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     7621 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     7681 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     7741 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     7801 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     7861 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     7921 tgccggctct gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt cggatctccc
     7981 tttggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     8041 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaaat
     8101 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     8161 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     8221 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     8281 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     8341 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     8401 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     8461 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     8521 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     8581 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     8641 ttttggaggc ctagactttt gcagagacgg cccaaattcg taatcatggt catagctgtt
     8701 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     8761 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     8821 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     8881 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     8941 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     9001 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     9061 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     9121 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     9181 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     9241 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     9301 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     9361 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     9421 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     9481 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     9541 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     9601 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     9661 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9721 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9781 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9841 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9901 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9961 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    10021 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    10081 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    10141 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    10201 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    10261 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    10321 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    10381 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    10441 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    10501 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    10561 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    10621 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    10681 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    10741 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    10801 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    10861 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
    10921 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
    10981 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
    11041 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
    11101 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
    11161 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
    11221 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
    11281 acgacgttgt aaaacgacgg ccagtgccaa gctg
//
