LOCUS       V043418                12592 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043418
VERSION     V043418
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6618
                     /label="NOS1(NM_000620)"
                     /note="NOS1(NM_000620)"
                     /gene="NOS1"
     CDS             6625..6690
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6734..6945
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6958..7226
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7255..7264
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7285..7947
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             8017..8070
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8071..8670
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8671..9259
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9333..9566
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9638..9759
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9799..9934
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9967..9983)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9991..10007
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10015..10045)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10060..10081
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10369..10957)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11128..11988)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11989..12093)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12567..12583
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg aggatcacat gttcggtgtt
     2341 cagcaaatcc agcccaatgt catttctgtt cgtctcttca agcgcaaagt tgggggcctg
     2401 ggatttctgg tgaaggagcg ggtcagtaag ccgcccgtga tcatctctga cctgattcgt
     2461 gggggcgccg cagagcagag tggcctcatc caggccggag acatcattct tgcggtcaac
     2521 ggccggccct tggtggacct gagctatgac agcgccctgg aggtactcag aggcattgcc
     2581 tctgagaccc acgtggtcct cattctgagg ggccctgaag gtttcaccac gcacctggag
     2641 accaccttta caggtgatgg gacccccaag accatccggg tgacacagcc cctgggtccc
     2701 cccaccaaag ccgtggatct gtcccaccag ccaccggccg gcaaagaaca gcccctggca
     2761 gtggatgggg cctcgggtcc cgggaatggg cctcagcatg cctacgatga tgggcaggag
     2821 gctggctcac tcccccatgc caacggcctg gcccccaggc ccccaggcca ggaccccgcg
     2881 aagaaagcaa ccagagtcag cctccaaggc agaggggaga acaatgaact gctcaaggag
     2941 atagagcctg tgctgagcct tctcaccagt gggagcagag gggtcaaggg aggggcacct
     3001 gccaaggcag agatgaaaga tatgggaatc caggtggaca gagatttgga cggcaagtca
     3061 cacaaacctc tgcccctcgg cgtggagaac gaccgagtct tcaatgacct atgggggaag
     3121 ggcaatgtgc ctgtcgtcct caacaaccca tattcagaga aggagcagcc ccccacctca
     3181 ggaaaacagt cccccacaaa gaatggcagc ccctccaagt gtccacgctt cctcaaggtc
     3241 aagaactggg agactgaggt ggttctcact gacaccctcc accttaagag cacattggaa
     3301 acgggatgca ctgagtacat ctgcatgggc tccatcatgc atccttctca gcatgcaagg
     3361 aggcctgaag acgtccgcac aaaaggacag ctcttccctc tcgccaaaga gtttattgat
     3421 caatactatt catcaattaa aagatttggc tccaaagccc acatggaaag gctggaagag
     3481 gtgaacaaag agatcgacac cactagcact taccagctca aggacacaga gctcatctat
     3541 ggggccaagc acgcctggcg gaatgcctcg cgctgtgtgg gcaggatcca gtggtccaag
     3601 ctgcaggtat tcgatgcccg tgactgcacc acggcccacg ggatgttcaa ctacatctgt
     3661 aaccatgtca agtatgccac caacaaaggg aacctcaggt ctgccatcac catattcccc
     3721 cagaggacag acggcaagca cgacttccga gtctggaact cccagctcat ccgctacgct
     3781 ggctacaagc agcctgacgg ctccaccctg ggggacccag ccaatgtgca gttcacagag
     3841 atatgcatac agcagggctg gaaaccgcct agaggccgct tcgatgtcct gccgctcctg
     3901 cttcaggcca acggcaatga ccctgagctc ttccagattc ctccagagct ggtgttggaa
     3961 gttcccatca ggcaccccaa gtttgagtgg ttcaaggacc tggggctgaa gtggtacggc
     4021 ctccccgccg tgtccaacat gctcctagag attggcggcc tggagttcag cgcctgtccc
     4081 ttcagtggct ggtacatggg cacagagatt ggtgtccgcg actactgtga caactcccgc
     4141 tacaatatcc tggaggaagt ggccaagaag atgaacttag acatgaggaa gacgtcctcc
     4201 ctgtggaagg accaggcgct ggtggagatc aatatcgcgg ttctctatag cttccagagt
     4261 gacaaagtga ccattgttga ccatcactcc gccaccgagt ccttcattaa gcacatggag
     4321 aatgagtacc gctgccgggg gggctgccct gccgactggg tgtggatcgt gccccccatg
     4381 tccggaagca tcacccctgt gttccaccag gagatgctca actaccggct caccccctcc
     4441 ttcgaatacc agcctgatcc ctggaacacg catgtctgga aaggcaccaa cgggaccccc
     4501 acaaagcggc gagccattgg cttcaagaag ctagcagaag ctgtcaagtt ctcggccaag
     4561 ctgatggggc aggctatggc caagagggtg aaagcgacca tcctctatgc cacagagaca
     4621 ggcaaatcgc aagcttatgc caagaccttg tgtgagatct tcaaacacgc ctttgatgcc
     4681 aaggtgatgt ccatggaaga atatgacatt gtgcacctgg aacatgaaac tctggtcctt
     4741 gtggtcacca gcacctttgg caatggagat ccccctgaga atggggagaa attcggctgt
     4801 gctttgatgg aaatgaggca ccccaactct gtgcaggaag aaaggaagag ctacaaggtc
     4861 cgattcaaca gcgtctcctc ctactctgac tcccaaaaat catcaggcga tgggcccgac
     4921 ctcagagaca actttgagag tgctggaccc ctggccaatg tgaggttctc agtttttggc
     4981 ctcggctcac gagcataccc tcacttttgc gccttcggac acgctgtgga caccctcctg
     5041 gaagaactgg gaggggagag gatcctgaag atgagggaag gggatgagct ctgtgggcag
     5101 gaagaggctt tcaggacctg ggccaagaag gtcttcaagg cagcctgtga tgtcttctgt
     5161 gtgggagatg atgtcaacat tgaaaaggcc aacaattccc tcatcagcaa tgatcgcagc
     5221 tggaagagaa acaagttccg cctcaccttt gtggccgaag ctccagaact cacacaaggt
     5281 ctatccaatg tccacaaaaa gcgagtctca gctgcccggc tccttagccg tcaaaacctc
     5341 cagagcccta aatccagtcg gtcaactatc ttcgtgcgtc tccacaccaa cgggagccag
     5401 gagctgcagt accagcctgg ggaccacctg ggtgtcttcc ctggcaacca cgaggacctc
     5461 gtgaatgccc tgatcgagcg gctggaggac gcgccgcctg tcaaccagat ggtgaaagtg
     5521 gaactgctgg aggagcggaa cacggcttta ggtgtcatca gtaactggac agacgagctc
     5581 cgcctcccgc cctgcaccat cttccaggcc ttcaagtact acctggacat caccacgcca
     5641 ccaacgcctc tgcagctgca gcagtttgcc tccctagcta ccagcgagaa ggagaagcag
     5701 cgtctgctgg tcctcagcaa gggtttgcag gagtacgagg aatggaaatg gggcaagaac
     5761 cccaccatcg tggaggtgct ggaggagttc ccatctatcc agatgccggc caccctgctc
     5821 ctgacccagc tgtccctgct gcagccccgc tactattcca tcagctcctc cccagacatg
     5881 taccctgatg aagtgcacct cactgtggcc atcgtttcct accgcactcg agatggagaa
     5941 ggaccaattc accacggcgt atgctcctcc tggctcaacc ggatacaggc tgacgaactg
     6001 gtcccctgtt tcgtgagagg agcacccagc ttccacctgc cccggaaccc ccaagtcccc
     6061 tgcatcctcg ttggaccagg caccggcatt gcccctttcc gaagcttctg gcaacagcgg
     6121 caatttgata tccaacacaa aggaatgaac ccctgcccca tggtcctggt cttcgggtgc
     6181 cggcaatcca agatagatca tatctacagg gaagagaccc tgcaggccaa gaacaagggg
     6241 gtcttcagag agctgtacac ggcttactcc cgggagccag acaaaccaaa gaagtacgtg
     6301 caggacatcc tgcaggagca gctggcggag tctgtgtacc gagccctgaa ggagcaaggg
     6361 ggccacatat acgtctgtgg ggacgtcacc atggctgctg atgtcctcaa agccatccag
     6421 cgcatcatga cccagcaggg gaagctctcg gcagaggacg ccggcgtatt catcagccgg
     6481 atgagggatg acaaccgata ccatgaggat atttttggag tcaccctgcg aacgtacgaa
     6541 gtgaccaacc gccttagatc tgagtccatt gccttcattg aagagagcaa aaaagacacc
     6601 gatgaggttt tcagctccgg atccgactac aaagaccatg acggtgatta taaagatcat
     6661 gacatcgact acaaggatga cgatgacaag tagcggccgc gaaggatctg cgatcgctcc
     6721 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     6781 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     6841 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     6901 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
     6961 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
     7021 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
     7081 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
     7141 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
     7201 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc
     7261 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc
     7321 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc
     7381 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc
     7441 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc
     7501 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac
     7561 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac
     7621 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc
     7681 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc
     7741 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac
     7801 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc
     7861 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac
     7921 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg
     7981 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca
     8041 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc
     8101 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     8161 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     8221 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     8281 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     8341 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     8401 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     8461 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     8521 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     8581 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     8641 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa
     8701 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     8761 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     8821 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     8881 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     8941 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     9001 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     9061 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     9121 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     9181 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     9241 ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga
     9301 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     9361 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     9421 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     9481 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     9541 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     9601 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     9661 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     9721 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     9781 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     9841 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     9901 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt
     9961 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
    10021 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
    10081 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
    10141 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
    10201 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
    10261 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    10321 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    10381 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    10441 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    10501 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    10561 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    10621 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    10681 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    10741 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    10801 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    10861 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    10921 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    10981 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    11041 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    11101 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    11161 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    11221 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    11281 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    11341 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    11401 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    11461 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    11521 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    11581 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    11641 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    11701 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    11761 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    11821 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    11881 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    11941 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    12001 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    12061 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    12121 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
    12181 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
    12241 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
    12301 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
    12361 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
    12421 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
    12481 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
    12541 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//
