LOCUS       V028195                10690 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028195
VERSION     V028195
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4716
                     /label="NOC3L(NM_022451)"
                     /note="NOC3L(NM_022451)"
                     /gene="NOC3L"
     CDS             4723..4788
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4832..5043
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5056..5324
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5353..5362
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5383..6045
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6115..6168
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6169..6768
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6769..7357
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7431..7664
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7736..7857
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7897..8032
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8065..8081)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8089..8105
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8113..8143)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8158..8179
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8467..9055)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9226..10086)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10087..10191)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10665..10681
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga aggcgagaag aaataaaaaa
     2341 cagatcccaa gctttcgcaa gttaataaaa actagtaaag tcaaacttga aaacaagcta
     2401 aaaaataagc agtttaaaca acaaagcact ctcaagaagt accgaaaaga acagaggaaa
     2461 ctaaggcaag ctgtgaaaga tgctgtgtct aagaaaccca ttccattgga gaacccaaag
     2521 gaaaagcgac caggtaaaag gattgagagg gaagaagagg aagaagaaga agcccttcct
     2581 ttagatatga tggatgaaga tgacttacag ttaatgaagg atttaggaca aagagtatct
     2641 tttctaacaa gagatctttc ttctagtgag cctgttcatg cgaagaaacg gaagcatgaa
     2701 cgcattatag ataaatatga aaaaatacca agaactctgc aaactgcacc agagaaggaa
     2761 ctgattcatt tacttcctat caaagataaa agtggtataa tcccacagac tagggagaag
     2821 ccagttactg atagtaacaa agatgaagag gatcaagaag aagagaggga acttgaggaa
     2881 gagatcattg aagatcctat tcaagagctg accatagaag aacatttgat tgagagaaag
     2941 aagaaattac aggagaagaa gatgcatatt gcagccttgg catctgccat attatcagat
     3001 ccagaaaata atattaaaaa attgaaagaa ttacgttcta tgttgatgga acaagatcct
     3061 gatgtggctg ttactgttcg aaagctggta attgtttctc tgatggagtt atttaaagat
     3121 attactcctt catataaaat ccggcccctc acagaagcag aaaaatctac taagacccga
     3181 aaagaaaccc agaagttaag agaatttgaa gaaggcctgg ttagccaata caagttttat
     3241 ttggaaaatc tggaacaaat ggttaaagat tggaagcaga ggaagctgaa gaaaagtaat
     3301 gtagtttcct taaaggcata caaaggactg gcagaagtcg ctgtgaagag cttgtgtgag
     3361 ctgttggtgg cactacctca ttttaacttt cacaacaaca tcatcgtatt gattgtccct
     3421 ctcatgaatg acatgtcaaa attgatatct gaaatgtgtt gtgaagctgt gaagaaactc
     3481 tttaagcaag ataaattagg ccaagcttct cttggtgtaa ttaaagtgat ttctggtttt
     3541 gtgaagggca gaaattacga agttaggcca gagatgttaa aaacattttt atgcctaaga
     3601 atcaaggaag tagaagtgaa aaaagataca gaagacatta ataaaccaaa aaaatttatg
     3661 actttcaaag aaaagagaaa atctctatca agaatgcaga gaaagtggaa gaaagcagaa
     3721 gagaaactag agcgagagct tcgagaggca gaagcttcag agagtactga gaaaaaactt
     3781 aaactgcaca cagagactct gaatattgtg tttgtaacct acttcagaat attgaagaag
     3841 gcccagaggt cacctctcct gccagcagtt ctagaaggtc ttgccaagtt tgctcacctt
     3901 ataaatgtgg aattttttga tgatctgtta gtagttcttc atactctcat tgagtctggt
     3961 gacctaagct atcaagaaag tcttcactgt gtccagactg cttttcatat tctttctgga
     4021 caaggtgatg ttctgaatat tgatccattg aaattctaca cacatctcta caaaacactg
     4081 ttcaaattac atgcaggtgc taccaatgaa ggtgttgaga ttgtactcca gtgccttgat
     4141 gtcatgctaa ctaagcgcag aaagcaagtt tctcagcagc gagctcttgc cttcatcaaa
     4201 cgcctttgta cccttgctct tcatgttctt ccaaattcaa gtattggcat tttagcaact
     4261 accagaatat taatgcatac tttccccaaa acagatctac tgcttgacag tgaatctcag
     4321 ggaagtggag ttttccttcc tgaactggat gagcctgagt actgcaatgc tcagaacact
     4381 gctctgtggg aactgcatgc tctgcggagg cattatcatc ccatagtgca gagatttgca
     4441 gcccacctga tcgctggagc accttctgaa ggctctggag cactcaaacc agagttgagt
     4501 cgaagatctg ctactgaact ttttgaggca tatagcatgg cagaaatgac attcaatcct
     4561 cctgttgaat cttcaaaccc caaaataaag ggtaaatttt tacaagggga ttcatttttg
     4621 aatgaagatt taaatcagct aatcaaaaga tactccagtg aagttgctac tgaatcgcct
     4681 ctggatttca cgaaatattt gaaaacatca ctacacggat ccgactacaa agaccatgac
     4741 ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta gcggccgcga
     4801 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     4861 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
     4921 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     4981 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     5041 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc
     5101 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     5161 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     5221 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
     5281 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg
     5341 cctacgctag acgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag
     5401 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc
     5461 acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc
     5521 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc
     5581 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc
     5641 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc
     5701 ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc
     5761 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat
     5821 aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc
     5881 ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac
     5941 gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc
     6001 atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag
     6061 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc
     6121 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac
     6181 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     6241 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     6301 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     6361 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     6421 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     6481 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     6541 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     6601 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     6661 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     6721 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg
     6781 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     6841 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     6901 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     6961 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     7021 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     7081 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     7141 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     7201 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     7261 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     7321 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa gaccaatgac
     7381 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     7441 aattcactcc caacgaagat aagatctgct ttttgcttgt actgggtctc tctggttaga
     7501 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7561 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7621 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     7681 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     7741 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7801 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7861 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7921 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7981 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt
     8041 tgcagagacc aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
     8101 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     8161 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     8221 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     8281 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8341 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8401 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8461 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8521 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8581 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8641 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8701 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8761 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8821 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8881 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     8941 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9001 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9061 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9121 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9181 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9241 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9301 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9361 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9421 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9481 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9541 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9601 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9661 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9721 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9781 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9841 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9901 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9961 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10021 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    10081 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    10141 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    10201 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
    10261 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
    10321 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
    10381 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
    10441 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
    10501 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
    10561 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
    10621 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
    10681 tgccaagctg
//
