LOCUS       V026678                11317 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026678
VERSION     V026678
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5343
                     /label="NLRP7(NM_139176)"
                     /note="NLRP7(NM_139176)"
                     /gene="NLRP7"
     CDS             5350..5415
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5459..5670
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5683..5951
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5980..5989
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6010..6672
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6742..6795
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6796..7395
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7396..7984
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8058..8291
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8363..8484
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8524..8659
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8692..8708)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8716..8732
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8740..8770)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8785..8806
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9094..9682)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9853..10713)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10714..10818)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11292..11308
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga catcgcccca gctagagtgg
     2341 actctgcaga cccttctgga gcagctgaac gaggatgaat taaagagttt caaatccctt
     2401 ttatgggctt ttcccctcga agacgtgcta cagaagaccc catggtctga ggtggaagag
     2461 gctgatggca agaaactggc agaaattctg gtcaacacct cctcagaaaa ttggataagg
     2521 aatgcgactg tgaacatctt ggaagagatg aatctcacgg aattgtgtaa gatggcaaag
     2581 gctgagatga tggaggacgg acaggtgcaa gaaatagata atcctgagct gggagatgca
     2641 gaagaagact cggagttagc aaagccaggt gaaaaggaag gatggagaaa ttcaatggag
     2701 aaacagtctt tggtctggaa gaacaccttt tggcaaggag acattgacaa tttccatgac
     2761 gacgtcactc tgagaaacca acggttcatt ccattcttga atcccagaac acccaggaag
     2821 ctaacacctt acacggtggt gctgcacggc cccgcaggcg tggggaaaac cacgctggcc
     2881 aaaaagtgta tgctggactg gacagactgc aacctcagcc cgacgctcag atacgcgttc
     2941 tacctcagct gcaaggagct cagccgcatg ggcccctgca gttttgcaga gctgatctcc
     3001 aaagactggc ctgaattgca ggatgacatt ccaagcatcc tagcccaagc acagagaatc
     3061 ctgttcgtgg tcgatggcct tgatgagctg aaagtcccac ctggggcgct gatccaggac
     3121 atctgcgggg actgggagaa gaagaagccg gtgcccgtcc tcctggggag tttgctgaag
     3181 aggaagatgt tacccagggc agccttgctg gtcaccacgc ggcccagggc actgagggac
     3241 ctccagctcc tggcgcagca gccgatctac gtaagggtgg agggcttcct ggaggaggac
     3301 aggagggcct atttcctgag acactttgga gacgaggacc aagccatgcg tgcctttgag
     3361 ctaatgagga gcaacgcggc cctgttccag ctgggctcgg cccccgcggt gtgctggatt
     3421 gtgtgcacga ctctgaagct gcagatggag aagggggagg acccggtccc cacctgcctc
     3481 acccgcacgg ggctgttcct gcgtttcctc tgcagccggt tcccgcaggg cgcacagctg
     3541 cggggcgcgc tgcggacgct gagcctcctg gccgcgcagg gcctgtgggc gcagatgtcc
     3601 gtgttccacc gagaggacct ggaaaggctc ggggtgcagg agtccgacct ccgtctgttc
     3661 ctggacggag acatcctccg ccaggacaga gtctccaaag gctgctactc cttcatccac
     3721 ctcagcttcc agcagtttct cactgccctg ttctacgccc tggagaagga ggagggggag
     3781 gacagggacg gccacgcctg ggacatcggg gacgtacaga agctgctttc cggagaagaa
     3841 agactcaaga accccgacct gattcaagta ggacacttct tattcggcct cgctaacgag
     3901 aagagagcca aggagttgga ggccactttt ggctgccgga tgtcaccgga catcaaacag
     3961 gaattgctgc aatgcaaagc acatcttcat gcaaataagc ccttatccgt gaccgacctg
     4021 aaggaggtct tgggctgcct gtatgagtct caggaggagg agctggcgaa ggtggtggtg
     4081 gccccgttca aggaaatttc tattcacctg acaaatactt ctgaagtgat gcattgttcc
     4141 ttcagcctga agcattgtca agacttgcag aaactctcac tgcaggtagc aaagggggtg
     4201 ttcctggaga attacatgga ttttgaactg gacattgaat ttgaaagctc aaacagcaac
     4261 ctcaagtttc tggaagtgaa acaaagcttc ctgagtgact cttctgtgcg gattctttgt
     4321 gaccacgtaa cccgtagcac ctgtcatctg cagaaagtgg agattaaaaa cgtcacccct
     4381 gacaccgcgt accgggactt ctgtcttgct ttcattggga agaagaccct cacgcacctg
     4441 accctggcag ggcacatcga gtgggaacgc acgatgatgc tgatgctgtg tgacctgctc
     4501 agaaatcata aatgcaacct gcagtacctg aggttgggag gtcactgtgc caccccggag
     4561 cagtgggctg aattcttcta tgtcctcaaa gccaaccagt ccctgaagca cctgcgtctc
     4621 tcagccaatg tgctcctgga tgagggtgcc atgttgctgt acaagaccat gacacgccca
     4681 aaacacttcc tgcagatgtt gtcgttggaa aactgtcgtc ttacagaagc cagttgcaag
     4741 gaccttgctg ctgtcttggt tgtcagcaag aagctgacac acctgtgctt ggccaagaac
     4801 cccattgggg atacaggggt gaagtttctg tgtgagggct tgagttaccc tgattgtaaa
     4861 ctgcagacct tggtgttaca gcaatgcagc ataaccaagc ttggctgtag atatctctca
     4921 gaggcgctcc aagaagcctg cagcctcaca aacctggact tgagtatcaa ccagatagct
     4981 cgtggattgt ggattctctg tcaggcatta gagaatccaa actgtaacct aaaacaccta
     5041 cgcctctgga gctgctccct catgcctttc tattgtcagc atcttggatc tgctctcctc
     5101 agcaatcaga agcttgaaac tctggacctg ggccagaatc atttgtggaa gagtggcata
     5161 attaagctct ttggggttct aagacaaaga actggatcct tgaagatact caggttgaag
     5221 acctatgaaa ctaatttgga aatcaagaag ctgttggagg aagtgaaaga aaagaatccc
     5281 aagctgacta ttgattgcaa tgcttccggg gcaacggcac ctccgtgctg tgactttttt
     5341 tgcggatccg actacaaaga ccatgacggt gattataaag atcatgacat cgactacaag
     5401 gatgacgatg acaagtagcg gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg
     5461 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg
     5521 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     5581 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     5641 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct
     5701 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg
     5761 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg
     5821 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc
     5881 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc
     5941 cgttacagat ccaagctgtg accggcgcct acgctagacg ccaccatgga gagcgacgag
     6001 agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag
     6061 ttcgagctgg tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg
     6121 aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac
     6181 ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc
     6241 aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac
     6301 gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc
     6361 accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc
     6421 gtggagcacc tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc
     6481 agcctgcgcg acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc
     6541 gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag
     6601 gagctgcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc
     6661 atcgccttcg ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga
     6721 cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag
     6781 gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     6841 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     6901 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     6961 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     7021 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     7081 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     7141 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     7201 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     7261 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     7321 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     7381 aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     7441 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     7501 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     7561 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     7621 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     7681 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     7741 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     7801 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     7861 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     7921 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     7981 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     8041 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagataag atctgctttt
     8101 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     8161 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     8221 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     8281 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     8341 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     8401 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     8461 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     8521 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     8581 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     8641 ggaggctttt ttggaggcct agacttttgc agagaccaaa ttcgtaatca tgtcatagct
     8701 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     8761 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     8821 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     8881 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     8941 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     9001 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     9061 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     9121 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     9181 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     9241 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     9301 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     9361 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     9421 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     9481 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     9541 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     9601 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     9661 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     9721 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     9781 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     9841 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     9901 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     9961 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    10021 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    10081 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    10141 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    10201 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    10261 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    10321 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    10381 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    10441 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    10501 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    10561 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    10621 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    10681 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    10741 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    10801 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
    10861 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
    10921 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
    10981 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
    11041 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
    11101 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc
    11161 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
    11221 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
    11281 gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
