LOCUS       V044966                14363 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044966
VERSION     V044966
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..3018
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             3055..9192
                     /label="NIN(NM_182944)"
                     /note="NIN(NM_182944)"
                     /gene="NIN"
     CDS             9202..9228
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             9232..9258
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             9262..9288
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        9333..9544
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             9557..9825
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             9852..10451
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    10458..11046
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             11120..11353
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    11425..11546
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      11565..11700
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(11738..11754)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11762..11778
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11786..11816)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11831..11852
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(12140..12728)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12899..13759)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13760..13864)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     14338..14354
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     2341 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     2401 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     2461 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     2521 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     2581 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     2641 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2701 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     2761 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     2821 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2881 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     2941 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     3001 atggacgagc tgtacaaggg cggcggaggg agtggaggag ggggaagtgg atccatggat
     3061 gaggtggagc aggaccagca tgaggcccga ctcaaggagc tgtttgacag ttttgacacg
     3121 acgggcacag ggtccctggg gcaggaggaa ctcaccgacc tttgccacat gttgagcttg
     3181 gaggaggtgg ccccagtgct gcagcagaca ttacttcagg acaacctctt gggcagggta
     3241 cattttgacc aatttaaaga agcattaata ctcatcttgt ccagaactct gtcaaatgaa
     3301 gaacactttc aagaaccaga ctgctcacta gaagctcagc ccaaatatgt tagaggtggg
     3361 aagcgttacg gacgaaggtc cttgcccgag ttccaagagt ccgtggagga gtttcctgaa
     3421 gtgacggtga ttgagccact ggatgaagaa gcgcggcctt cacacatccc agccggtgac
     3481 tgcagtgagc actggaagac gcaacgcagt gaggagtatg aagcggaagg ccagttaagg
     3541 ttttggaacc cagatgactt gaatgcttca cagagtggat cttcccctcc ccaagactgg
     3601 atagaagaga aactgcaaga agtttgtgaa gatttgggga tcacccgtga tggtcacctg
     3661 aaccggaaga agctggtctc catctgtgag cagtatggtt tacagaatgt ggatggagag
     3721 atgctcgagg aagtattcca taatcttgat cctgacggta caatgagtgt agaagatttt
     3781 ttctatggtt tgtttaaaaa tggaaaatct cttacaccat cagcatctac tccatataga
     3841 caactaaaaa ggcacctttc catgcagtct ttcgatgaga gtggacgacg taccacaacc
     3901 tcatcagcaa tgacaagtac cattggcttt cgggtcttct cctgcctgga tgatgggatg
     3961 ggccatgcat ctgtggagag aatactggac acctggcagg aagagggcat tgagaacagc
     4021 caggagatcc tgaaggcctt ggatttcagc ctcgatggaa acatcaattt gacagaatta
     4081 acactggccc ttgaaaatga acttttggtt accaagaaca gcattcacca ggcggctctg
     4141 gccagcttta aggctgaaat ccggcatttg ttggaacgag ttgatcaggt ggtcagagaa
     4201 aaagagaagc tacggtcaga tctggacaag gccgagaagc tcaagtcttt aatggcctcg
     4261 gaggtggatg atcaccatgc ggccatagag cggcggaatg agtacaacct caggaaactg
     4321 gatgaagagt acaaggagcg aatagcagcc ttaaaaaatg aactccgaaa agagagagag
     4381 cagatcctgc agcaggcagg caagcagcgt ttagaacttg aacaggaaat tgaaaaggca
     4441 aaaacagaag agaactatat ccgggaccgc cttgccctct ctttaaagga aaacagtcgt
     4501 ctggaaaatg agcttctaga aaatgcagag aagttggcag aatatgagaa tctgacaaac
     4561 aaacttcaga gaaatttgga aaatgtgtta gcagaaaagt ttggtgacct cgatcctagc
     4621 agtgctgagt tcttcctgca agaagagaga ctgacacaga tgagaaatga atatgagcgg
     4681 cagtgcaggg tactacaaga ccaagtagat gaactccagt ctgagctgga agaatatcgt
     4741 gcacaaggca gagtgctcag gcttccgttg aagaactcac cgtcagaaga agttgaggct
     4801 aacagcggtg gcattgagcc cgaacacggg ctcggttctg aagaatgcaa tccattgaat
     4861 atgagcattg aggcagagct ggtcattgaa cagatgaaag aacaacatca cagggacata
     4921 tgttgcctca gactggagct cgaagataaa gtgcgccatt atgaaaagca gctggacgaa
     4981 accgtggtca gctgcaagaa ggcacaggag aacatgaagc aaaggcatga gaacgaaacg
     5041 cacaccttag aaaaacaaat aagtgacctt aaaaatgaaa ttgctgaact tcaggggcaa
     5101 gcagcagtgc tcaaggaggc acatcatgag gccacttgca ggcatgagga ggagaaaaaa
     5161 caactgcaag tgaagcttga ggaggaaaag actcacctgc aggagaagct gaggctgcaa
     5221 catgagatgg agctcaaggc tagactgaca caggctcaag caagctttga gcgggagagg
     5281 gaaggccttc agagtagcgc ctggacagaa gagaaggtga gaggcttgac tcaggaacta
     5341 gagcagtttc accaggagca gctgacaagc ctggtggaga aacacactct tgagaaagag
     5401 gagttaagaa aagagctctt ggaaaagcac caaagggagc ttcaggaggg aagggaaaaa
     5461 atggaaacag agtgtaatag aagaacctct caaatagaag cccagtttca gtctgattgt
     5521 cagaaagtca ctgagaggtg tgaaagcgct ctgcaaagcc tggaggggcg ctaccgccaa
     5581 gagctgaagg acctccagga acagcagcgt gaggagaaat cccagtggga atttgagaag
     5641 gacgagctca cccaggagtg tgcggaagcc caggagctgc tgaaagagac tcttaagaga
     5701 gagaaaacaa cttctctggt cctgacccag gagagagaga tgctggagaa aacatacaaa
     5761 gaacatttga acagcatggt cgtcgagaga cagcagctac tccaagacct ggaagaccta
     5821 agaaatgtat ctgaaaccca gcaaagcctg ctgtctgacc agatacttga gctgaagagc
     5881 agtcacaaaa gggaactgag ggagcgtgag gaggtcctgt gccaggcagg ggcttcggag
     5941 cagctggcca gccagcggct ggaaagacta gaaatggaac atgaccagga aaggcaggaa
     6001 atgatgtcca agcttctagc catggagaac attcacaaag cgacctgtga gacagcagat
     6061 cgagaaagag ccgagatgag cacagaaatc tccagacttc agagtaaaat aaaggaaatg
     6121 cagcaggcaa catctcctct ctcaatgctt cagagtggtt gccaggtgat aggagaggag
     6181 gaggtggaag gagatggagc cctgtccctg cttcagcaag gggagcagct gttggaagaa
     6241 aatggggacg tcctcttaag cctgcagaga gctcatgaac aggcagtgaa ggaaaatgtg
     6301 aaaatggcta ctgaaatttc tagattgcaa cagaggctac aaaagttaga gccagggtta
     6361 gtaatgtctt cttgtttgga tgagccagct actgagtttt ttggaaatac tgcggaacaa
     6421 acagagcagt ttttacagca aaaccgaacg aagcaagtag aaggtgtgac caggcggcat
     6481 gtcctaagtg acctggaaga tgatgaggtc cgggacctgg gaagtacagg gacgagctct
     6541 gttcagagac aggaagtcaa aatagaggag tctgaagctt cagtagaggg tttttctgag
     6601 cttgaaaaca gtgaagagac caggactgaa tcctgggagc tgaagaatca gattagtcag
     6661 cttcaggaac agctaatgat gttatgtgcg gactgtgatc gagcttctga aaagaaacag
     6721 gacctacttt ttgatgtttc tgtgctaaaa aagaaactga agatgcttga gagaatccct
     6781 gaggcttctc ccaaatataa gctgttgtat gaagatgtga gccgagaaaa tgactgcctt
     6841 caggaagagc tgagaatgat ggagacacgc tacgatgagg cactagaaaa taacaaagaa
     6901 ctcactgcag aggttttcag gttgcaggat gagctgaaga aaatggagga agtcactgaa
     6961 acattcctca gcctggaaaa gagttacgat gaggtcaaaa tagaaaatga ggggctgaat
     7021 gttctggttt tgagacttca aggcaagatt gagaagcttc aggaaagcgt ggtccagcgg
     7081 tgtgactgct gcttatggga agccagttta gagaacctgg aaatcgaacc tgatggaaat
     7141 atactccagc tcaatcagac actggaagag tgtgtgccca gggttaggag tgtacatcat
     7201 gtcatagagg aatgtaagca agaaaaccag taccttgagg ggaacacaca gctcttggaa
     7261 aaagtaaaag cacatgaaat tgcctggtta catggaacaa ttcagacaca tcaagaaagg
     7321 ccaagagtac agaatcaagt tatactggag gaaaacacta ctctcctagg ctttcaagac
     7381 aaacattttc agcatcaggc caccatagca gagttagaac tggagaaaac aaagttacag
     7441 gagctgacta ggaagttgaa ggagagagtc actattttag ttaagcaaaa agatgtactt
     7501 tctcacggag aaaaggagga agagctgaag gcaatgatgc atgacttgca gatcacgtgc
     7561 agtgagatgc agcaaaaagt tgaacttctg agatatgaat ctgaaaagct tcaacaggaa
     7621 aattctattt tgagaaatga aattactact ttaaatgaag aagatagcat ttctaacctg
     7681 aaattaggga cattaaatgg atctcaggaa gaaatgtggc aaaaaacgga aactgtaaaa
     7741 caagaaaatg ctgcagttca gaagatggtt gaaaatttaa agaaacagat ttcagaatta
     7801 aaaatcaaaa accaacaatt ggatttggaa aatacagaac ttagccaaaa gaactctcaa
     7861 aaccaggaaa aactgcaaga acttaatcaa cgtctaacag aaatgctatg ccagaaggaa
     7921 aaagagccag gaaacagtgc attggaggaa cgggaacaag agaagtttaa tctgaaagaa
     7981 gaactggaac gttgtaaagt gcagtcctcc actttagtgt cttctctgga ggcggagctc
     8041 tctgaagtta aaatacagac ccatattgtg caacaggaaa accaccttct caaagatgaa
     8101 ctggagaaaa tgaaacagct gcacagatgt cccgatctct ctgacttcca gcaaaaaatc
     8161 tctagtgttc taagctacaa cgaaaaactg ctgaaagaaa aggaagctct gagtgaggaa
     8221 ttaaatagct gtgtcgataa gttggcaaaa tcaagtcttt tagagcatag aattgcgacg
     8281 atgaagcagg aacagaaatc ctgggaacat cagagtgcga gcttaaagtc acagctggtg
     8341 gcttctcagg aaaaggttca gaatttagaa gacaccgtgc agaatgtaaa cctgcaaatg
     8401 tcccggatga aatctgacct acgagtgact cagcaggaaa aggaggcttt aaaacaagaa
     8461 gtgatgtctt tacataagca acttcagaat gctggtggca agagctgggc cccagagata
     8521 gctactcatc catcagggct ccataaccag cagaaaaggc tgtcctggga caagttggat
     8581 catctgatga atgaggaaca gcagctgctt tggcaagaga atgagaggct ccagaccatg
     8641 gtacagaaca ccaaagccga actcacgcac tcccgggaga aggtccgtca gttggaatcc
     8701 aatcttcttc ccaagcacca aaaacatcta aacccatcag gtaccatgaa tcccacagag
     8761 caagaaaaat tgagcttaaa gagagagtgt gatcagtttc agaaagaaca atctcctgct
     8821 aacaggaagg tcagtcagat gaattccctt gaacaagaat tagaaacaat tcatttggaa
     8881 aatgaaggcc tgaaaaagaa acaagtaaaa ctggatgagc agctcatgga gatgcagcac
     8941 ctgaggtcca ctgcgacgcc tagcccgtcc cctcatgctt gggatttgca gctgctccag
     9001 cagcaagcct gtccgatggt gcccagggag cagtttctgc agcttcaacg ccagctgctg
     9061 caggcagaaa ggataaacca gcacctgcag gaggaacttg aaaacaggac ctccgaaacc
     9121 aacacaccac aggccttgct accggagcag cgagcagtgc acgcagattc ctacagaagg
     9181 attgggcacc tggcctcgag ttacccatac gacgtacctg attatgcaac ttatccttat
     9241 gatgtacctg attatgctag ctatccttat gatgtcccag actacgcata ggcggccgcg
     9301 aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     9361 cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt
     9421 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     9481 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     9541 acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg
     9601 ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac
     9661 tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct
     9721 cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca
     9781 actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc
     9841 gcctacgcta gatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     9901 cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     9961 tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
    10021 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
    10081 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
    10141 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
    10201 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
    10261 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
    10321 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
    10381 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
    10441 ccggtgcctg agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
    10501 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
    10561 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
    10621 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
    10681 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
    10741 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
    10801 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
    10861 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
    10921 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
    10981 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
    11041 ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
    11101 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt
    11161 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
    11221 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
    11281 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
    11341 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
    11401 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
    11461 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    11521 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
    11581 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
    11641 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
    11701 tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa
    11761 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
    11821 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
    11881 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    11941 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
    12001 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    12061 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    12121 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    12181 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    12241 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    12301 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    12361 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    12421 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    12481 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    12541 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
    12601 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    12661 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    12721 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    12781 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    12841 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    12901 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    12961 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    13021 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    13081 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    13141 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    13201 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    13261 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    13321 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    13381 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    13441 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    13501 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    13561 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    13621 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    13681 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    13741 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    13801 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    13861 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
    13921 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
    13981 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
    14041 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
    14101 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
    14161 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
    14221 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
    14281 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
    14341 aaacgacggc cagtgccaag ctg
//
