LOCUS       V028477                10990 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028477
VERSION     V028477
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5016
                     /label="NFKB2(NM_001077494)"
                     /note="NFKB2(NM_001077494)"
                     /gene="NFKB2"
     CDS             5023..5088
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5132..5343
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5356..5624
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5653..5662
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5683..6345
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6415..6468
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6469..7068
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7069..7657
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7731..7964
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8036..8157
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8197..8332
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8365..8381)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8389..8405
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8413..8443)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8458..8479
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8767..9355)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9526..10386)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10387..10491)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10965..10981
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg agagttgcta caacccaggt
     2341 ctggatggta ttattgaata tgatgatttc aaattgaact cctccattgt ggaacccaag
     2401 gagccagccc cagaaacagc tgatggcccc tacctggtga tcgtggaaca gcctaagcag
     2461 agaggcttcc gatttcgata tggctgtgaa ggcccctccc atggaggact gcccggtgcc
     2521 tccagtgaga agggccgaaa gacctatccc actgtcaaga tctgtaacta cgagggacca
     2581 gccaagatcg aggtggacct ggtaacacac agtgacccac ctcgtgctca tgcccacagt
     2641 ctggtgggca agcaatgctc ggagctgggg atctgcgccg tttctgtggg gcccaaggac
     2701 atgactgccc aatttaacaa cctgggtgtc ctgcatgtga ctaagaagaa catgatgggg
     2761 actatgatac aaaaacttca gaggcagcgg ctccgctcta ggccccaggg ccttacggag
     2821 gccgagcagc gggagctgga gcaagaggcc aaagaactga agaaggtgat ggatctgagt
     2881 atagtgcggc tgcgcttctc tgccttcctt agagccagtg atggctcctt ctccctgccc
     2941 ctgaagccag tcatctccca gcccatccat gacagcaaat ctccgggggc atcaaacctg
     3001 aagatttctc gaatggacaa gacagcaggc tctgtgcggg gtggagatga agtttatctg
     3061 ctttgtgaca aggtgcagaa agatgacatt gaggttcggt tctatgagga tgatgagaat
     3121 ggatggcagg cctttgggga cttctctccc acagatgtgc ataaacagta tgccattgtg
     3181 ttccggacac ccccctatca caagatgaag attgagcggc ctgtaacagt gtttctgcaa
     3241 ctgaaacgca agcgaggagg ggacgtgtct gattccaaac agttcaccta ttaccctctg
     3301 gtggaagaca aggaagaggt gcagcggaag cggaggaagg ccttgcccac cttctcccag
     3361 cccttcgggg gtggctccca catgggtgga ggctctgggg gtgcagccgg gggctacgga
     3421 ggagctggag gaggtggcag cctcggtttc ttcccctcct ccctggccta cagcccctac
     3481 cagtccggcg cgggccccat gggctgctac ccgggaggcg ggggcggggc gcagatggcc
     3541 gccacggtgc ccagcaggga ctccggggag gaagccgcgg agccgagcgc cccctccagg
     3601 accccccagt gcgagccgca ggccccggag atgctgcagc gagctcgaga gtacaacgcg
     3661 cgcctgttcg gcctggcgca gcgcagcgcc cgagccctac tcgactacgg cgtcaccgcg
     3721 gacgcgcgcg cgctgctggc gggacagcgc cacctgctga cggcgcagga cgagaacgga
     3781 gacacaccac tgcacctagc catcatccac gggcagacca gtgtcattga gcagatagtc
     3841 tatgtcatcc accacgccca ggacctcggc gttgtcaacc tcaccaacca cctgcaccag
     3901 acgcccctgc acctggcggt gatcacgggg cagacgagtg tggtgagctt tctgctgcgg
     3961 gtaggtgcag acccagctct gctggatcgg catggagact cagccatgca tctggcgctg
     4021 cgggcaggcg ctggtgctcc tgagctgctg cgtgcactgc ttcagagtgg agctcctgct
     4081 gtgccccagc tgttgcatat gcctgacttt gagggactgt atccagtaca cctggcggtc
     4141 cgagcccgaa gccctgagtg cctggatctg ctggtggaca gtggggctga agtggaggcc
     4201 acagagcggc aggggggacg aacagccttg catctagcca cagagatgga ggagctgggg
     4261 ttggtcaccc atctggtcac caagctccgg gccaacgtga acgctcgcac ctttgcggga
     4321 aacacacccc tgcacctggc agctggactg gggtacccga ccctcacccg cctccttctg
     4381 aaggctggtg ctgacatcca tgctgaaaac gaggagcccc tgtgcccact gccttcaccc
     4441 cctacctctg atagcgactc ggactctgaa gggcctgaga aggacacccg aagcagcttc
     4501 cggggccaca cgcctcttga cctcacttgc agcaccaagg tgaagacctt gctgctaaat
     4561 gctgctcaga acaccatgga gccacccctg accccgccca gcccagcagg gccgggactg
     4621 tcacttggtg atacagctct gcagaacctg gagcagctgc tagacgggcc agaagcccag
     4681 ggcagctggg cagagctggc agagcgtctg gggctgcgca gcctggtaga cacgtaccga
     4741 cagacaacct cacccagtgg cagcctcctg cgcagctacg agctggctgg cggggacctg
     4801 gcaggtctac tggaggccct gtctgacatg ggcctagagg agggagtgag gctgctgagg
     4861 ggtccagaaa cccgagacaa gctgcccagc acagcagagg tgaaggaaga cagtgcgtac
     4921 gggagccagt cagtggagca ggaggcagag aagctgggcc caccccctga gccaccagga
     4981 gggctctgcc acgggcaccc ccagcctcag gtgcacggat ccgactacaa agaccatgac
     5041 ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta gcggccgcga
     5101 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     5161 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
     5221 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     5281 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     5341 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc
     5401 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     5461 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     5521 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
     5581 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg
     5641 cctacgctag acgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag
     5701 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc
     5761 acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc
     5821 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc
     5881 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc
     5941 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc
     6001 ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc
     6061 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat
     6121 aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc
     6181 ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac
     6241 gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc
     6301 atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag
     6361 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc
     6421 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac
     6481 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     6541 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     6601 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     6661 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     6721 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     6781 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     6841 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     6901 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     6961 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     7021 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg
     7081 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     7141 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     7201 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     7261 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     7321 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     7381 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     7441 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     7501 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     7561 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     7621 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa gaccaatgac
     7681 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     7741 aattcactcc caacgaagat aagatctgct ttttgcttgt actgggtctc tctggttaga
     7801 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7861 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7921 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     7981 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     8041 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     8101 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     8161 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     8221 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     8281 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt
     8341 tgcagagacc aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
     8401 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     8461 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     8521 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     8581 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8641 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8701 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8761 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8821 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8881 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8941 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9001 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     9061 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9121 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9181 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     9241 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9301 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9361 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9421 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9481 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9541 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9601 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9661 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9721 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9781 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9841 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9901 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9961 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    10021 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    10081 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    10141 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10201 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    10261 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10321 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    10381 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    10441 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    10501 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
    10561 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
    10621 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
    10681 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
    10741 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
    10801 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
    10861 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
    10921 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
    10981 tgccaagctg
//
