LOCUS       V035388                10414 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035388
VERSION     V035388
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4440
                     /label="NEK5(NM_199289)"
                     /note="NEK5(NM_199289)"
                     /gene="NEK5"
     CDS             4447..4512
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4556..4767
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4780..5048
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5077..5086
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5107..5769
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5839..5892
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5893..6492
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6493..7081
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7155..7388
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7460..7581
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7621..7756
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7789..7805)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7813..7829
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7837..7867)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7882..7903
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8191..8779)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8950..9810)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9811..9915)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10389..10405
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ataagtacga tgtgattaag
     2341 gccatcgggc aaggtgcctt cgggaaagca tacttagcta aagggaaatc agatagcaag
     2401 cactgtgtca taaaagagat caattttgaa aagatgccca tacaagaaaa agaagcttca
     2461 aagaaagaag tgattcttct ggaaaagatg aaacatccca acattgtagc cttcttcaat
     2521 tcatttcaag agaatggcag gctgtttatt gtaatggaat attgtgatgg aggggatctc
     2581 atgaaaagga tcaatagaca acggggtgtg ttatttagtg aagatcagat cctcggttgg
     2641 tttgtacaga tttctctagg actaaaacat attcatgaca ggaagatatt acacagggac
     2701 ataaaagctc agaacatttt tcttagcaag aacggaatgg tggcaaagct tggggacttt
     2761 ggtatagcaa gagtcctgaa taattccatg gaacttgctc gaacttgtat tggaacacct
     2821 tactacctgt ccccagagat ctgtcagaat aaaccctaca acaataaaac ggatatttgg
     2881 tctcttggct gtgtcttata tgagctctgc acacttaaac atccttttga gggtaacaac
     2941 ttacagcagc tggttctgaa gatttgtcaa gcacattttg ccccaatatc tccggggttt
     3001 tctcgtgagc tccattcctt gatatctcag ctctttcaag tatctcctcg agaccgacca
     3061 tccataaatt ccattttgaa aaggcccttt ttagagaatc ttattcccaa atatttgact
     3121 cctgaggtca ttcaggaaga attcagtcac atgcttatat gcagagcagg agcgccagct
     3181 tctcgacatg ctgggaaggt ggtccagaag tgtaaaatac aaaaagtgag attccaggga
     3241 aagtgcccac caagatcaag gatatctgtg ccaattaaaa ggaatgctat attgcataga
     3301 aatgaatgga gaccaccagc tggagcccag aaggccagat ctataaaaat gatagaaaga
     3361 cccaaaattg ctgctgtctg tggacattat gattattatt atgctcaact tgatatgctg
     3421 aggaggagag cccacaaacc aagttatcac cctattcctc aagaaaatac tggagttgag
     3481 gattacggtc aggaaacgag gcatggtcca tccccaagtc aatggcctgc tgagtacctt
     3541 cagagaaaat ttgaagctca acaatataag ttgaaagtgg agaagcaatt gggtcttcgt
     3601 ccatcttctg ccgagccaaa ttacaaccag agacaagagc taagaagtaa tggagaagag
     3661 cctagattcc aggagctgcc atttaggaaa aacgaaatga aggaacagga atattggaag
     3721 cagttagagg aaatacgcca acagtaccac aatgacatga aagaaattag aaagaagatg
     3781 gggagagaac cagaggagaa ctcaaaaata agtcataaaa cctatttggt gaagaagagt
     3841 aacctgcctg tccatcaaga tgcatctgag ggagaagcac ctgtgcagat ggaatttcgc
     3901 tcttgttgcc caggctggag tgcaatggca cgatcttggc tcaccgcaac ctccgcctcc
     3961 caggacattg aaaaagactt gaaacaaatg aggcttcaga acacaaagga aagtaaaaat
     4021 ccagaacaga aatataaagc taagaagggg gtaaaatttg aaattaattt agacaaatgt
     4081 atttctgatg aaaacatcct ccaagaggaa gaggcaatgg atataccaaa tgaaactttg
     4141 acctttgagg atggcatgaa gtttaaggaa tatgaatgtg taaaggagca tggagattat
     4201 acagacaaag catttgaaaa acttcactgc ccagaagcag ggttttccac gcagactgta
     4261 gctgctgtgg gaaacaggag gcagtgggat ggaggagcgc ctcagactct gctgcagatg
     4321 atggcagtgg ccgacatcac ctccacctgc cccacggggc ctgacagtga gtctgtgctt
     4381 agcgtcagtc gtcaggaagg gaagaccaag gacccgtaca gcccagtgct catcctgatg
     4441 ggatccgact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
     4501 gacgatgaca agtagcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca
     4561 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt
     4621 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     4681 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     4741 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca
     4801 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct
     4861 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
     4921 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
     4981 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
     5041 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc
     5101 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc
     5161 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag
     5221 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc
     5281 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac
     5341 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg
     5401 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc
     5461 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg
     5521 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc
     5581 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc
     5641 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag
     5701 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc
     5761 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc
     5821 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag
     5881 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     5941 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     6001 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     6061 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     6121 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     6181 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     6241 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     6301 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     6361 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     6421 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     6481 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     6541 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6601 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     6661 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     6721 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     6781 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     6841 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     6901 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     6961 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     7021 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     7081 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     7141 agaaaagggg ggactggaag ggctaattca ctcccaacga agataagatc tgctttttgc
     7201 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7261 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7321 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7381 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     7441 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     7501 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7561 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     7621 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     7681 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     7741 ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt catagctgtt
     7801 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     7861 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     7921 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     7981 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     8041 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     8101 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8161 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8221 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8281 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8341 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8401 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8461 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8521 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8581 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     8641 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8701 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8761 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8821 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8881 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8941 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9001 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9061 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9121 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9181 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9241 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9301 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9361 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9421 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9481 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9541 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9601 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9661 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9721 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9781 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9841 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9901 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     9961 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
    10021 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
    10081 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
    10141 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
    10201 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
    10261 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
    10321 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
    10381 acgacgttgt aaaacgacgg ccagtgccaa gctg
//
