LOCUS       V039292                11327 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039292
VERSION     V039292
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..6159
                     /label="NEK1(NM_001199397)"
                     /note="NEK1(NM_001199397)"
                     /gene="NEK1"
     CDS             6166..6192
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             6196..6222
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             6226..6252
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        6297..6508
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6521..6789
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6816..7415
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7422..8010
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8084..8317
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8389..8510
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8529..8664
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8702..8718)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8726..8742
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8750..8780)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8795..8816
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9104..9692)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9863..10723)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10724..10828)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11302..11318
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggagaag tatgttagac tacagaagat tggagaaggt
     2341 tcatttggaa aagccattct tgttaaatct acagaagatg gcagacagta tgttatcaag
     2401 gaaattaaca tctcaagaat gtccagtaaa gaaagagaag aatcaaggag agaagttgca
     2461 gtattggcaa acatgaagca tccaaatatt gtccagtata gagaatcatt tgaagaaaat
     2521 ggctctctct acatagtaat ggattactgt gagggagggg atctgtttaa gcgaataaat
     2581 gctcagaaag gcgttttgtt tcaagaggat cagattttgg actggtttgt acagatatgt
     2641 ttggccctga aacatgtaca tgatagaaaa attcttcatc gagacattaa atctcagaac
     2701 atatttttaa ctaaagatgg aacagtacaa cttggagatt ttggaattgc tagagttctt
     2761 aatagtactg tagagctggc tcgaacttgc atagggaccc catactactt gtcacctgaa
     2821 atctgtgaaa acaaacctta caataataaa agtgacattt gggctctggg gtgtgtcctt
     2881 tatgagctgt gtacacttaa acatgctttt gaagctggca gtatgaaaaa cctggtactg
     2941 aagataatat ctggatcttt tccacctgtg tctttgcatt attcctatga tctccgcagt
     3001 ttggtgtctc agttatttaa aagaaatcct agggatagac catcagtcaa ctccatattg
     3061 gagaaaggtt ttatagccaa acgcattgaa aagtttctct ctcctcagct tattgcagaa
     3121 gaattttgtc taaaaacatt ttcgaagttt ggatcacagc ctataccagc taaaagacca
     3181 gcttcaggac aaaactcgat ttctgttatg cctgctcaga aaattacaaa gcctgccgct
     3241 aaatatggaa tacctttagc atataagaaa tatggagata aaaaattaca cgaaaagaaa
     3301 ccactgcaaa aacataaaca ggcccatcaa actccagaga agagagtgaa tactggagaa
     3361 gaaaggagga aaatatctga ggaagcagca agaaagagaa ggctggaatt tattgaaaaa
     3421 gaaaagaaac aaaaggatca gattattagt ttaatgaagg ctgaacaaat gaaaaggcaa
     3481 gaaaaggaaa ggttggaaag aataaatagg gccagggaac aaggatggag aaatgtgcta
     3541 agtgctggtg gaagtggtga agtaaaggct ccttttctgg gcagtggagg gactatagct
     3601 ccatcatctt tttcttctcg aggacagtat gaacattacc atgccatttt tgaccaaatg
     3661 cagcaacaaa gagcagaaga taatgaagct aaatggaaaa gagaaatata tggtcgaggt
     3721 cttccagaaa gaggaattct gcctggagtt cgtccaggat ttccttatgg ggctgcaggt
     3781 catcaccatt ttcctgatgc tgatgatatt agaaaaactt tgaaaagatt gaaggcggtg
     3841 tctaaacaag ccaatgcaaa caggcaaaaa gggcagctag ctgtagaaag agctaaacaa
     3901 gtagaagagt tcctgcagcg aaaacgggaa gctatgcaga ataaagctcg agccgaagga
     3961 catatgggaa tcctgcaaaa cctggcagct atgtatggag gcaggcccag ctcttcaaga
     4021 ggagggaagc caagaaacaa agaggaagag gtttatctgg caagactgag gcaaataaga
     4081 ctacagaatt tcaatgagcg ccaacagatt aaagccaaac ttcgtggtga aaagaaagaa
     4141 gctaatcatt ctgaaggaca agaaggaagt gaagaggctg acatgaggcg caaaaaaatc
     4201 gaatcactga aggcccatgc aaatgcacgt gctgctgtac taaaagaaca actagaacga
     4261 aagagaaagg aggcttatga gagagaaaaa aaagtgtggg aagagcattt ggtggctaaa
     4321 ggagttaaga gttctgatgt ttctccacct ttgggacagc atgaaacagg tggctctcca
     4381 tcaaagcaac agatgagatc tgttatttct gtaacttcag ctttgaaaga agttggcgtg
     4441 gacagtagtt taactgatac ccgggaaact tcagaagaga tgcaaaagac caacaatgct
     4501 atttcaagta agcgagaaat acttcgtaga ttaaatgaaa atcttaaagc tcaagaagat
     4561 gaaaaaggaa agcagaatct ctctgatact tttgagataa atgttcatga agatgccaaa
     4621 gagcatgaaa aagaaaaatc agtttcatct gatcgcaaga agtgggaggc aggaggtcaa
     4681 cttgtgattc ctctggatga gttaacacta gatacatcct tctctacaac tgaaagacat
     4741 acagtgggag aagttattaa attaggtcct aatggatctc caagaagagc ctgggggaaa
     4801 agtccgacag attctgttct aaagatactt ggagaagctg aactacaact tcagacagaa
     4861 ctattagaaa atacaactat tagaagtgag atttctcccg aaggggaaaa gtacaaaccc
     4921 ttaattactg gagaaaaaaa agtacaatgt atttcacatg aaataaaccc atcagctatt
     4981 gttgattctc ctgttgagac aaaaagtccc gagttcagtg aggcatctcc acagatgtca
     5041 ttgaaactgg aaggaaattt agaagaacct gatgatttgg aaacagaaat tctacaagag
     5101 ccaagtggaa caaacaaaga tgagagcttg ccatgcacta ttactgatgt gtggattagt
     5161 gaggaaaaag aaacaaagga aactcagtcg gcagatagga tcaccattca ggaaaatgaa
     5221 gtttctgaag atggagtctc gagtactgtg gaccaactta gtgacattca tatagagcct
     5281 ggaaccaatg attctcagca ctctaaatgt gatgtagata agtctgtgca accggaacca
     5341 tttttccata aggtggttca ttctgaacac ttgaacttag tccctcaagt tcaatcagtt
     5401 cagtgttcac cagaagaatc ctttgcattt cgatctcact cgcatttacc accaaaaaat
     5461 aaaaacaaga attccttgct gattggactt tcaactggtc tgtttgatgc aaacaaccca
     5521 aagatgttaa ggacatgttc acttccagat ctctcaaagc tgttcagaac ccttatggat
     5581 gttcccaccg taggagatgt tcgtcaagac aatcttgaaa tagatgaaat tgaagatgaa
     5641 aacattaaag aaggaccttc tgattctgaa gacattgtgt ttgaagaaac tgacacagat
     5701 ttacaagagc tgcaggcctc gatggaacag ttacttaggg aacaacctgg tgaagaatac
     5761 agtgaagaag aagagtcagt cttgaagaac agtgatgtgg agccaactgc aaatgggaca
     5821 gatgtggcag atgaagatga caatcccagc agtgaaagtg ccctgaacga agaatggcac
     5881 tcagataaca gtgatggtga aattgctagt gaatgtgaat gcgatagtgt ctttaaccat
     5941 ttagaggaac tgagacttca tctggagcag gaaatgggct ttgaaaaatt ctttgaggtt
     6001 tatgagaaaa taaaggctat tcatgaagat gaagatgaaa atattgaaat ttgttcaaaa
     6061 atagttcaaa atattttggg aaatgaacat cagcatcttt atgccaagat tcttcattta
     6121 gtcatggcag atggagccta ccaagaagat aatgatgaag gatcctaccc atacgacgta
     6181 cctgattatg caacttatcc ttatgatgta cctgattatg ctagctatcc ttatgatgtc
     6241 ccagactacg cataggcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc
     6301 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg
     6361 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     6421 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     6481 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc
     6541 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc
     6601 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc
     6661 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac
     6721 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg
     6781 ttacagatcc aagctgtgac cggcgcctac gctagatgac cgagtacaag cccacggtgc
     6841 gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg
     6901 actaccccgc cacgcgccac accgtcgacc cggaccgcca catcgagcgg gtcaccgagc
     6961 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     7021 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     7081 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     7141 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     7201 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     7261 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     7321 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     7381 gcacctggtg catgacccgc aagcccggtg cctgagtcga caatcaacct ctggattaca
     7441 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     7501 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     7561 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     7621 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     7681 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     7741 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     7801 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     7861 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     7921 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     7981 gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat gacttacaag
     8041 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     8101 tcccaacgaa aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     8161 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     8221 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     8281 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     8341 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     8401 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     8461 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     8521 ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     8581 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     8641 agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat
     8701 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     8761 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     8821 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     8881 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     8941 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     9001 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     9061 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     9121 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     9181 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     9241 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     9301 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     9361 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     9421 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9481 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9541 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9601 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9661 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9721 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     9781 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     9841 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     9901 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     9961 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    10021 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    10081 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    10141 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    10201 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    10261 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    10321 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    10381 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    10441 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    10501 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    10561 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    10621 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    10681 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    10741 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    10801 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
    10861 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
    10921 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
    10981 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
    11041 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
    11101 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
    11161 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
    11221 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
    11281 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
