LOCUS V027134 10513 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V027134 VERSION V027134 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2317..4539 /label="NCAPH(NM_015341)" /note="NCAPH(NM_015341)" /gene="NCAPH" CDS 4546..4611 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 4655..4866 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4879..5147 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 5176..5185 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 5206..5868 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 5938..5991 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 5992..6591 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6592..7180 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7254..7487 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 7559..7680 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 7720..7855 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7888..7904) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7912..7928 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7936..7966) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7981..8002 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8290..8878) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9049..9909) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9910..10014) /gene="bla" /label="AmpR promoter" primer_bind 10488..10504 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg gacctcccgg cccagcactg 2341 ccagccacaa tgaataactc ttcttcagag acgcgaggac acccccacag tgcctcctct 2401 ccttcagagc gtgtgttccc gatgcccctg cccaggaagg cgcctctcaa tattcctggc 2461 accccagtcc tcgaagactt tcctcagaat gacgatgaga aggagcggct gcagcggagg 2521 cgctcgaggg tctttgatct gcagttcagc actgactcac ctcgcttatt ggcctccccc 2581 tccagcagga gtattgacat ttcagctact atccccaagt ttacaaacac gcagattacg 2641 gaacattact ccacctgtat caaactgtcc actgaaaata aaatcactac caagaatgct 2701 tttggtttgc acttgattga ttttatgtca gagattctta aacagaaaga caccgaacca 2761 accaacttta aagtggctgc gggtactctg gatgccagca ccaagatcta tgctgtgcgc 2821 gtggatgccg tccatgccga tgtatacaga gtccttgggg ggctgggcaa agatgcaccg 2881 tctttggaag aagtagaagg ccatgttgct gatggaagtg ctactgaaat gggaacaacc 2941 aaaaaggctg taaagccaaa gaagaagcac ttacacagaa ctattgagca gaacataaac 3001 aacctcaatg tctccgaagc agatcggaag tgtgagattg atcccatgtt tcagaagaca 3061 gcagcctcat ttgatgagtg cagcacagca ggggtgtttc tgtccactct ccactgccag 3121 gactacagaa gtgaactgct gtttccctct gatgtccaga ctctctccac gggagaacct 3181 ctcgagttgc cagagttagg ttgtgtagaa atgacagatt taaaagcgcc cttgcagcag 3241 tgtgcagaag atcgccagat ctgcccttcc ctggccgggt tccagtttac acagtgggac 3301 agtgaaacac ataatgagtc tgtgtcggcc ctggtagaca agtttaagaa gaatgaccag 3361 gtatttgaca tcaatgctga agttgacgag agtgactgtg gagacttccc cgatgggtcc 3421 ctgggggatg actttgatgc caacgatgaa cctgaccaca ccgcagttgg ggatcatgaa 3481 gagttcagga gctggaagga gccctgccag gttcagagct gccaggaaga aatgatttcc 3541 cttggggatg gagacatcag gaccatgtgc ccccttctgt ctatgaaacc tggagaatat 3601 tcttatttca gtcctcggac catgtcgatg tgggctggcc cggatcactg gcgctttagg 3661 cctcgacgca aacaagatgc tccttcccaa tcagaaaaca aaaagaagag tacaaaaaaa 3721 gattttgaaa ttgactttga agatgatatt gactttgatg tatattttag aaaaacaaag 3781 gctgctacta ttctgaccaa gtccactttg gagaaccaga attggagagc taccaccctt 3841 cctacagatt tcaactacaa tgttgacact ctggtccagc ttcacctcaa accaggcacc 3901 aggttactta agatggccca gggccatagg gtagagactg agcattatga agaaattgaa 3961 gactatgatt acaacaaccc taacgacacc tccaactttt gccctggatt acaggctgct 4021 gacagtgatg atgaagattt ggatgactta tttgtgggac ctgttgggaa ctctgacctc 4081 tcaccttatc cttgccatcc acctaagaca gcacaacaga atggtgacac tccagaagcc 4141 caaggattag acatcacaac atatggggag tcaaacttgg tagctgagcc tcagaaggta 4201 aataaaattg aaattcacta tgccaagact gccaaaaaga tggacatgaa gaaactgaag 4261 cagagcatgt ggagtctgct gacagcgctc tccggaaagg aggcagatgc agaggcaaac 4321 cacagggaag ctggaaaaga agcggccctg gcagaagtgg ctgacgagaa gatgcttagc 4381 gggctcacga aggacctgca gaggagcctg ccccctgtca tggctcagaa cctctccata 4441 cctctggctt ttgcctgtct cctacattta gccaatgaaa agaatctaaa actggaagga 4501 acagaggacc tctctgatgt tcttgtgagg caaggagatg gatccgacta caaagaccat 4561 gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa gtagcggccg 4621 cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc 4681 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg 4741 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa 4801 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga 4861 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga 4921 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga 4981 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg 5041 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct 5101 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg 5161 gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc 5221 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag 5281 ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc 5341 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac 5401 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc 5461 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag 5521 gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg 5581 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc 5641 gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac 5701 agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag 5761 aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg 5821 ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct 5881 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag 5941 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag 6001 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc 6061 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc 6121 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 6181 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 6241 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 6301 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 6361 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 6421 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 6481 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 6541 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct 6601 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 6661 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 6721 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 6781 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 6841 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 6901 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 6961 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt 7021 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 7081 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc 7141 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat 7201 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg 7261 gctaattcac tcccaacgaa gataagatct gctttttgct tgtactgggt ctctctggtt 7321 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 7381 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 7441 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg 7501 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa 7561 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 7621 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 7681 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc 7741 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 7801 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac 7861 ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc 7921 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 7981 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 8041 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 8101 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 8161 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 8221 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 8281 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 8341 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 8401 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 8461 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 8521 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 8581 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 8641 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 8701 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 8761 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 8821 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 8881 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 8941 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 9001 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 9061 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 9121 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 9181 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 9241 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 9301 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 9361 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 9421 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 9481 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 9541 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 9601 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 9661 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 9721 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 9781 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 9841 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 9901 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 9961 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 10021 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 10081 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct 10141 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 10201 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc 10261 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 10321 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga 10381 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc 10441 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc 10501 cagtgccaag ctg //