LOCUS       V027246                11299 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027246
VERSION     V027246
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..5341
                     /label="NCAPG(NM_022346)"
                     /note="NCAPG(NM_022346)"
                     /gene="NCAPG"
     CDS             5348..5413
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5458..5669
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5682..5950
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5979..5988
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6009..6671
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6741..6794
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6795..7394
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7395..7982
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8056..8289
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8361..8482
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8501..8636
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8674..8690)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8698..8714
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8722..8752)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8767..8788
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9076..9664)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9835..10695)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10696..10800)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11274..11290
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgg gagcggaaag gaggctgctg tcgattaagg aggcctttcg
     2341 gctggcgcag cagccgcacc agaaccaggc gaagctggtg gtggcgctga gccgcaccta
     2401 ccgcacgatg gatgataaga cagtttttca tgaggagttc attcattacc ttaaatatgt
     2461 tatggtggtc tataaacgtg aaccagctgt ggagagggta atagaatttg cagcaaagtt
     2521 tgttacctca tttcaccaat cagatatgga agatgatgag gaagaggaag atggtggcct
     2581 tttaaattat ttgtttactt ttctcttaaa gtctcatgaa gcaaacagca atgcagtgag
     2641 atttagagtg tgcctgctca taaacaagct tttgggaagt atgccagaaa atgctcagat
     2701 tgatgatgat gtgtttgata aaattaataa agccatgctt attagattga aagataagat
     2761 tccaaatgtg agaatacagg cagttctggc gctttcacga cttcaggatc ccaaggatga
     2821 tgaatgccca gtggttaatg catatgctac tttgattgaa aatgattcaa atccagaagt
     2881 tagacgggca gtgttatcat gtattgcacc atcagcaaag actttgccaa aaattgtagg
     2941 gcgcaccaag gatgtgaaag aggctgtcag aaagctggct tatcaggttt tagctgaaaa
     3001 ggttcatatg agagctatgt ccattgctca gagagtaatg ctccttcaac aaggtcttaa
     3061 tgacagatca gatgctgtga aacaagctat gcagaagcat cttcttcaag gctggttacg
     3121 gttctctgaa ggaaatatct tagagttgct ccatcggttg gatgtagaaa attcttctga
     3181 agtggcagtc tctgttctca atgccttgtt ttcaataact cctctcagtg aactggtggg
     3241 actctgtaaa aacaatgatg gcaggaaatt gattccagtg gaaacattaa ctcctgaaat
     3301 tgctttgtat tggtgtgccc tttgtgaata tttgaaatca aaaggagatg aaggtgaaga
     3361 atttttagag cagattttgc cagagcctgt agtatatgca gactatttat tgagttacat
     3421 ccagagcatt ccagttgtta atgaagaaca cagaggtgat ttttcctata ttggaaattt
     3481 gatgacaaaa gaattcatag gtcaacaatt gattctaatt attaagtctt tggataccag
     3541 tgaagaagga ggaagaaaaa aactgctggc tgttttacag gagattctta ttttacccac
     3601 aatcccaata tccctggttt cttttcttgt tgaaagacta ctccacatca ttatagatga
     3661 taataagaga acacaaattg ttacagaaat tatctcagag attcgggcgc ccattgttac
     3721 tgttggtgtt aataacgatc cagctgatgt aagaaagaaa gaactcaaga tggctgaaat
     3781 aaaagttaag cttatcgaag ccaaagaagc tttggaaaat tgcattacct tacaggattt
     3841 taatcgggca tcagaattaa aagaagaaat aaaagcatta gaagatgcca gaataaacct
     3901 tttgaaagag acagagcaac ttgaaattaa agaagtccac atagagaaga atgatgctga
     3961 aacattgcag aaatgtctta ttttatgcta tgaactgttg aagcagatgt ccatttcaac
     4021 aggcttaagt gcaaccatga atggaatcat cgaatctttg attcttcctg gaataataag
     4081 tattcatcct gttgtaagaa acctggctgt tttatgcttg ggatgctgtg gactacagaa
     4141 tcaggatttt gcaaggaaac acttcgtatt actattgcag gttttgcaaa ttgatgatgt
     4201 cacaataaaa ataagtgctt taaaggcaat ctttgaccaa ctgatgacgt tcgggattga
     4261 accatttaaa actaaaaaaa tcaaaacact tcattgtgaa ggtacagaaa taaacagtga
     4321 tgatgagcaa gaatcaaaag aagttgaaga gactgctaca gctaagaatg ttctgaaact
     4381 cctttctgat ttcttagata gtgaggtatc tgaacttagg actggagctg cagaaggact
     4441 agccaagctg atgttctctg ggcttttggt cagcagcagg attctttctc gtcttatttt
     4501 gttatggtac aatcctgtga ctgaagagga tgttcaactt cgacattgcc taggcgtgtt
     4561 cttccccgtg tttgcttatg caagcaggac taatcaggaa tgctttgaag aagcttttct
     4621 tccaaccctg caaacactgg ccaatgcccc tgcatcttct cctttagctg aaattgatat
     4681 cacaaatgtt gctgagttac ttgtagattt gacaagacca agtggattaa atcctcaggc
     4741 caagacttcc caagattatc aggccttaac agtacatgac aatttggcta tgaaaatttg
     4801 caatgagatc ttaacaagtc cgtgctcgcc agaaattcga gtctatacaa aagccttgag
     4861 ttctttagaa ctcagtagcc atcttgcaaa agatcttctg gttctattga atgagattct
     4921 ggagcaagta aaagatagga catgtctgag agctttggag aaaatcaaga ttcagttaga
     4981 aaaaggaaat aaagaatttg gtgaccaagc tgaagcagca caggatgcca ccttgactac
     5041 aactactttc caaaatgaag atgaaaagaa taaagaagta tatatgactc cactcagggg
     5101 tgtaaaagca acccaagcat caaagtctac tcagctaaag actaacagag gacagagaaa
     5161 agtgacagtt tcagctagga cgaacaggag gtgtcagact gctgaagccg actctgaaag
     5221 tgatcatgaa gttccagaac cagaatcaga aatgaagatg agactaccaa gacgagccaa
     5281 aaccgcagca ctagaaaaaa gtaaacttaa ccttgcccaa tttctcaatg aagatctaag
     5341 tggatccgac tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga
     5401 tgacgatgac aagtgagcgg ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg
     5461 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaacgg
     5521 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     5581 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     5641 ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt
     5701 cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc
     5761 ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt
     5821 cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca
     5881 cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc
     5941 gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag agcgacgaga
     6001 gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt
     6061 tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc aacaagatga
     6121 agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg atgggctacg
     6181 gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg cacgccatca
     6241 acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc gtgctgcacg
     6301 tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca
     6361 ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc aacgccaccg
     6421 tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc cgcaccttca
     6481 gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac ttcaagagcg
     6541 ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc cgcgtggagg
     6601 agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc aagaccccca
     6661 tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc accgccggac
     6721 ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt gacgtggagg
     6781 agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     6841 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     6901 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     6961 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     7021 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     7081 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     7141 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     7201 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     7261 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     7321 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     7381 agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     7441 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     7501 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     7561 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     7621 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     7681 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     7741 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     7801 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     7861 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     7921 ctcttccgcg tctccgcctt cgccctcaga cgagtcggat ctccctttgg ccgcctcccc
     7981 gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     8041 aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat ctgctttttg
     8101 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     8161 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     8221 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     8281 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     8341 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     8401 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     8461 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     8521 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8581 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga
     8641 cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     8701 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8761 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8821 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8881 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8941 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     9001 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     9061 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     9121 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     9181 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     9241 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     9301 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     9361 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     9421 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     9481 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     9541 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9601 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9661 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9721 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9781 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9841 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9901 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9961 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    10021 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    10081 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    10141 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    10201 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    10261 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    10321 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    10381 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    10441 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    10501 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    10561 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    10621 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10681 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10741 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10801 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
    10861 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
    10921 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    10981 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
    11041 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
    11101 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
    11161 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
    11221 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
    11281 gacggccagt gccaagctg
//
