LOCUS       V040506                11065 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040506
VERSION     V040506
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5091
                     /label="NACA(NM_001113203)"
                     /note="NACA(NM_001113203)"
                     /gene="NACA"
     CDS             5098..5163
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5207..5418
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5431..5699
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5728..5737
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5758..6420
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6490..6543
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6544..7143
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7144..7732
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7806..8039
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8111..8232
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8272..8407
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8440..8456)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8464..8480
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8488..8518)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8533..8554
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8842..9430)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9601..10461)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10462..10566)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11040..11056
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc ccggcgaagc cacagaaacc
     2341 gtccctgcta cagagcagga gttgccgcag ccccaggctg agacagctgt gctacctatg
     2401 tcttcagcct tgagtgtcac tgctgcctta gggcagcctg gacctaccct cccccctcct
     2461 tgctctcctg ccccacaaca gtgccctctc tcagctgcta accaggcttc cccattccct
     2521 tccccctcta ctattgcctc gaccccttta gaagttcctt ttccccagtc atcctctgga
     2581 acagccctac ctttgggaac tgcccctgaa gccccaacct tcctaccaaa cctaataggg
     2641 cctcccatct ccccagctgc cttagctcta gcctctccca tgatagctcc aactctgaaa
     2701 gggacccctt cctcttcagc tcccttagct ctggttgccc tggctcccca ctcagttcag
     2761 aagagttctg cttttccacc taaccttctt acttcacctc cttcagtggc tgtagctgag
     2821 tcagggtcag tgataactct gtcagctccc attgctccct cagaaccaaa gactaatctt
     2881 aataaagttc cctctgaggt agtccctaat ccaaaaggca cccccagccc tccatgtata
     2941 gtcagtactg ttccttacca ctgtgtgact cccatggcct ctattcaatc tggagtggcc
     3001 tcccttcctc agacaacacc cacaactacc ctagccatcg cttcccctca agtcaaagat
     3061 accaccattt cctcagttct gatttctcca caaaacccag gaagcctcag cctgaagggg
     3121 cctgttagtc cacctgctgc cttatctctt tcaactcagt ctcttcctgt ggtgacctct
     3181 tctcaaaaga ctgcgggtcc caacaccccc ccagattttc ccatttctct gggctctcat
     3241 cttgcacctt tacatcagag ttcttttggt tctgtccaac ttttaggtca aacaggtcct
     3301 agtgctttgt cagaccctac agtgaagacc atttctgtag atcattcttc cacaggggcc
     3361 tcttatcctt ctcagagatc tgtaattcct ccccttcctt ccagaaatga ggtagttcct
     3421 gctactgtgg ctgcctttcc agtggtggct ccatctgttg acaaaggtcc ctctaccatc
     3481 tctagcataa cctgcagccc ttctggctcc ttaaatgtag ctacctcttt ttcattatct
     3541 cctacaacct ctctcattct caaaagctct cctaatgcca cttatcatta tcctttagtg
     3601 gcccaaatgc ccgtttcttc tgttggaacc accccacttg tggtgactaa cccctgtaca
     3661 attgctgcag cacctactac tacctttgag gtagctactt gtgtttctcc tccaatgtca
     3721 tcaggtccca taagtaacat agaaccaact tcccctgctg ccttggttat ggcacctgtg
     3781 gctcccaaag agccttctac tcaagtagca accactctga ggataccagt ctctcctcct
     3841 ctgccagacc ctgaagacct caaaaatctc cccagttcag tattggttaa atttccaaca
     3901 caaaaagacc tccaaactgt acctgcctct cttgaaggag cccctttctc tccagcccaa
     3961 gcaggactca ccaccaagaa agaccctact gtattaccgt tagtccaggc agcccctaaa
     4021 aattcccctt ctttccaaag tacatcctct tctccagaga tacctctttc tcctgaagcc
     4081 accctagcaa agaaaagcct tggggagcct ctccctatag tggctgcatt tcctttggaa
     4141 agtgctgacc ctgccggggt ggctcccaca actgccaaag cagctgcctt tgagaaggtc
     4201 cttcctaaac ctgaatcagc atctgtctct gcagcaccct ccccaccagt ctctctgcct
     4261 cttgctccct ccccagttcc cactctgcct cctaaacagc aatttctgcc gtcctctcct
     4321 gggctggtgt tggaatcacc ctctaaaccc cttgcccctg ctgatgagga tgagctgctg
     4381 cctctgattc ccccggaacc aatctctggg ggagtgcctt tccagtcggt cctcgtcaac
     4441 atgcccaccc ctaaatctgc tggaatccct gtcccaaccc cctctgccaa gcaacctgtt
     4501 acgaagaaca acaaggggtc tggaacagaa tctgacagtg atgaatcagt accagagctt
     4561 gaagaacagg attccaccca ggcaaccaca caacaagccc agctggcggc agcagctgaa
     4621 attgatgaag aaccagtcag taaagcaaaa cagagtcgga gtgaaaagaa ggcacggaag
     4681 gctatgtcca aactgggtct tcggcaggtt acaggagtta ctagagtcac tatccggaaa
     4741 tctaagaata tcctctttgt catcacaaaa ccagatgtct acaagagccc tgcttcagat
     4801 acttacatag tttttgggga agccaagatc gaagatttat cccagcaagc acaactagca
     4861 gctgctgaga aattcaaagt tcaaggtgaa gctgtctcaa acattcaaga aaacacacag
     4921 actccaactg tacaagagga gagtgaagag gaagaggtcg atgaaacagg tgtagaagtt
     4981 aaggacattg aattggtcat gtcacaagca aatgtgtcga gagcaaaggc agtccgagcc
     5041 ctgaagaaca acagtaatga tattgtaaat gcgattatgg aattaacaat gggatccgac
     5101 tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga tgacgatgac
     5161 aagtagcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca
     5221 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga
     5281 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     5341 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     5401 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc
     5461 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt
     5521 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc
     5581 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg
     5641 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc
     5701 aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag cggcctgccc
     5761 gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg
     5821 ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa
     5881 ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac
     5941 ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc
     6001 tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc
     6061 taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc
     6121 gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg
     6181 caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac
     6241 ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc
     6301 agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc
     6361 aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat cgccttcgcc
     6421 agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc
     6481 ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc
     6541 cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     6601 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     6661 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     6721 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     6781 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     6841 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     6901 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     6961 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     7021 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     7081 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     7141 tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     7201 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     7261 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     7321 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     7381 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     7441 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     7501 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     7561 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     7621 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     7681 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggtacc
     7741 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     7801 gggactggaa gggctaattc actcccaacg aagataagat ctgctttttg cttgtactgg
     7861 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7921 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7981 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8041 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     8101 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     8161 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     8221 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     8281 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     8341 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     8401 ggaggcctag acttttgcag agaccaaatt cgtaatcatg tcatagctgt ttcctgtgtg
     8461 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     8521 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     8581 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     8641 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     8701 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8761 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8821 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8881 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8941 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     9001 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     9061 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     9121 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     9181 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     9241 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     9301 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     9361 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     9421 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     9481 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     9541 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     9601 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9661 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9721 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9781 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9841 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9901 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9961 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    10021 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    10081 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    10141 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    10201 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    10261 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    10321 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    10381 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    10441 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    10501 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    10561 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
    10621 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
    10681 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
    10741 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
    10801 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
    10861 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
    10921 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
    10981 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
    11041 taaaacgacg gccagtgcca agctg
//
