LOCUS       V032918                13324 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032918
VERSION     V032918
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..7350
                     /label="N4bp2(NM_001024917)"
                     /note="N4bp2(NM_001024917)"
                     /gene="N4bp2"
     CDS             7357..7422
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        7466..7677
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7690..7958
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7987..7996
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             8017..8679
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             8749..8802
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8803..9402
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    9403..9991
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             10065..10298
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    10370..10491
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      10531..10666
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10699..10715)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10723..10739
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10747..10777)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10792..10813
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11101..11689)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11860..12720)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12721..12825)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     13299..13315
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg attgtctgtt agaattgtct
     2341 gccactgatg ccaagggggc agagtcgtct gcacagactt ctggtgatcg tgacagcaag
     2401 cctggtgcag caggaaactc agtaatggag acgtgccatc ccgaaggcga gggtgaagat
     2461 tccaagctgg attcattttt ggatatgcag ctaactgagg acttggattc cttaatacag
     2521 aacgcttttg agaaattaaa ctctcctcct gatgaccaag tatacccatt ctcacctctg
     2581 caagatgcta atagttttaa cgacccttcc acctttatga actcagattc aagtggcatg
     2641 acttctcttc tttctgtaca gagcacgagc tcaaacagcg aaactctcaa gagttctgct
     2701 tctttaccag gttcaaaccc accaacttca cattcagttt tgaatgcgcc tgagagttgt
     2761 gcaaaattgg cattggaagg gggctactca gaaggctctc ctctcagtag ctctggaaat
     2821 ccaacaaatg gctctgttag aggttgtggc aattttagcc aaagacagaa agatctttca
     2881 gaatctgagt gtcctagtgc gcagcattct caagcccttg cagatttggg caacagtgac
     2941 cctcaggctc cttcaactca ccctctgcac aattcagggt ctgacctacc aggtacagat
     3001 ggggatcaga agtcggcttc tgcgcctgat gtttttgtgc cctctgaagg gttcaatttc
     3061 aagccgcaca aacatcctga actgccacca aaggggaagg atatgaatta ctgcccggtg
     3121 ctcacccctc ttcccttact ccttccgccc ccgcccccac caccgatctg gaacccgatg
     3181 atccctgctt ttgatctctt tcaaggaaac catggctttg tagcccctgt ggtgaccaca
     3241 gctgctcact ggagacctgt caactacacg ttcccacccc ccatcatctc ccacaactct
     3301 ccaaccaaag tgtggagagg cggtgaggga gccagtgctt accaggtgca ggaggcccca
     3361 gcgtcacagc ctgtgaggaa gaaggctacc tccttcgttg gcttagttct cgtgcttctc
     3421 agagggctgc cgggctcagg gaaatccttt ctggcaagga ctttgcaaga ggataaccca
     3481 ggtggcgtca ttctcagcac tgatgactac ttctatataa acggacagta ccagtttgat
     3541 gtgaagtact taggggaagc ccatgagtgg aaccagaacc gagcaaaaga agcatttgag
     3601 aagaaggttt ctccagtaat catagataat acaaacctcc aagcgtggga aatgaagcca
     3661 tatgttgctt tgtctcaaaa gcataaatat aaggtccttt tccgggagcc agacacgtgg
     3721 tggaagttca agccgaagga gcttgcgaga cgtaatattc atggagtaag caaagagaaa
     3781 atatcaagaa tgctggagca ctatcagcgc tttgtctccg tgccgataat catgagctct
     3841 tccgatccag agaaaaccga gcgcattgag ttgtgtgcat acgcttctga cgacagccca
     3901 agagacagtg aagctattag ctctgcaaaa gaagacagtg tctcatctgc gtctccgaag
     3961 cactcagagc taacggaaga gaagaccctt gaggtggcca cacaagcagt gttatctgtg
     4021 cgtgatcccc agctcccttg tcctggttta aacagaggaa gaaaggagtt gggtgacgtg
     4081 agtcacggtg gtcataaccc cttctttcag gaagccccga gcacctattt tcctaaccct
     4141 gaaagcaaag gtcaagccac agatagaagt gaggaagaac aagagatggt gtctgaaaaa
     4201 gaacattgtg aagcagatgc gtggaggcct gcggggggag cgtcatcaga tgggcgctgt
     4261 gctgatgata atcaagaagc ctgtgggtct gcaggtgcag ccgcgcttcc aggtgagacc
     4321 ccctcgcctc ctgaaatgct ggaagaaaga acggcaggaa agaaaaagac cattggaaaa
     4381 caaaaaggca aatcatcttt ggaaaaaatc cccaaacaag agctatcaaa ttttgttggt
     4441 gactggccag ttgataagac aattagccag agaaccaaaa ggaaccggaa aactgaaaaa
     4501 ggcttatctg tacaaagtga caaaaaaggt aacctcccgc agtcccacaa agtattagat
     4561 gctagggtct ctgtgaatac agatcctgtc cagccccaag gatcttcaca tgggaacgac
     4621 gatctttcag aagtgcccag tagctatcac tatgatcctt acagaagtac cgagcaaagc
     4681 tcacttagtg ctgtgagcga ctggccttcc tctgcttcct tagctccgag agagcacaga
     4741 ccgagaatgc caaaagctga cttaagtgaa cccagcgtgg aatttggaac taatgacaac
     4801 ctgggtgaaa tacccttata tccagcacac gaggcctact gggggacaag ccctgaggag
     4861 ctgaagacgt tgagttccag ccttcgaagt tctgagatgc tgcccagtca aacagctcct
     4921 gagcatcatc cgacctccct gaatccactg gctcacagcc agcacgcgct gcccctgacc
     4981 ttccccagta gtgcagcagc tcttcctgga gtagtgggtc cccggtctct aacagaattt
     5041 ccagtggggg tgtctggaat tgtctctgga gtagatacag gcacttgtac ccagactgaa
     5101 ccccaagatt ttgctctctt gtggaaaata gaaaagaata aaatcaatgt ttcagattct
     5161 gtcagagtgt tgacaggaag attggatggg tttaagccca aagatttcac tcttaataga
     5221 aaagtaaatg tccaagaaac aattccctac agagtaatgc atgataaaag tacattcgtt
     5281 gaagaaagtg agcttaccag tgctgatgag tctgaaaatc ttaacatcct ttgcaaacta
     5341 ttcggatcct tttccttaga agccttgaag gatttatatg agcgatgtaa taaagacatt
     5401 atttgggcca caagtctctt gttggattct gagactaaac tgtgcgagga cactgcggtt
     5461 gagagctccc ccaagtcgta cagcgagtca caggttgggc cgttttctat gggcttggac
     5521 ctgaaggaaa ttattagcca cagaggaacc tcagaaggtt ctaattcttc tgtatcagaa
     5581 tttagtcctg gaattggtat ccggaacacc agtgcacggt ctgctggtaa cccaggaaag
     5641 gggacctcag agcaggaagg gagaagagct atgaatcctg aaaaccctga attaataact
     5701 agagtattcc ccaatgctgc tgtaaaggtc aagagtaata atgagacact gcctgaccgt
     5761 caggctgaac tgccaggcgc gtacaccttt aagcagcctc tttcacttac tccacgatct
     5821 tatatcccta aagatgttag tgagatagaa aaaaatctag taatgacaga gactagagac
     5881 aatatgcatt catttctaaa tttatctgat attataaact ctgcaacaag cacttcaaat
     5941 cctgaattaa atgaagatgt ttatcttact ggctctttgg aagtaaagaa aaatgaaaat
     6001 cttcctaagg attatgtgaa atttgcaaac atggaagaat ttatcaatga agataaacag
     6061 gaaatggaga aaaatctaat gccaggaagt ggttggtcag caggagttag tgaagagggt
     6121 aaagctgagg tattgactcc cacgccagtg acagccacat ctctgaccat agactgtctg
     6181 gagctggcgt taccccctga gctggctttc caacttaatg agctgtttgg cccagttggt
     6241 attgattcag ggtctctaac agtcgaggac tgtgtggttc atatagatct gaatctggct
     6301 aaagtgattc atgagaaatg gagagaatct gtaatggagc gacagagaca ggaggaggtt
     6361 tctcgaggca agcgcacaca agatcctttg ctggctggac atactggtct tgataatttt
     6421 gaacaaaaat catctcagaa aacaggcaaa aggttattga agactttagc agcacctgag
     6481 accctggatc actggaacac tcaaacaaaa aaagtttcac ttcgagaaat aatgtcagaa
     6541 gaaattgcct tacaggagaa acatgacttg aaaagggaga cacttatgtt tgaaaaagac
     6601 tgtgccacaa aactaaagga aaagcagctc tttaaaatat ttccagccat taatcaaaat
     6661 ttcctggtgg acattttcaa agatcacagc tactcactgg aaaacactgt acagtttcta
     6721 aactgtgtgc ttgaaggaga ccctgtgaaa acagttgtag ctcaagagtg tgttcaccaa
     6781 aatgagaata acacttatac tgcacagaaa tcaaaagaga aaaaggcaaa gaaaccgaaa
     6841 gaggctgagg acagcccagg tgacccatct ttccaggatt ttgagtaccc tgagtatgat
     6901 gactacaggg cagaagcttt cctgcaccag cagaagagga tggagtgcta cagcaaggcc
     6961 aaggaagcct accgcatggg gaagaagaac gtggccacct tctacgctca gcagggcagt
     7021 cttcatgagc agaagatgaa agaagccaat cacctcgctg ctgtggagat ctttgagaaa
     7081 gtcaacgcct ctctgctacc gcagaacgtc ttagacctcc atgggctgca tgtggatgaa
     7141 gctatagaac atttgacagc agttctacag cagaaaacag aagaatttaa acagagtggt
     7201 ggcaagccgt atctgtcggt gattactggg agaggaaatc acagccaggg aggagttgct
     7261 cgcatcaaac cagctgtcat taaatacctc acaagccaca gcttcaggtt ctctgaaatt
     7321 aagccagggt gcttgaaagt catgctaaag ggatccgact acaaagacca tgacggtgat
     7381 tataaagatc atgacatcga ctacaaggat gacgatgaca agtagcggcc gcgaaggatc
     7441 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     7501 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg
     7561 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     7621 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg
     7681 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat
     7741 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc
     7801 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg
     7861 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac
     7921 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg
     7981 ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc
     8041 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc
     8101 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc
     8161 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc
     8221 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag
     8281 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc
     8341 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc
     8401 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg
     8461 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg
     8521 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc
     8581 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg
     8641 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc
     8701 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga
     8761 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc
     8821 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     8881 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     8941 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     9001 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     9061 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     9121 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     9181 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     9241 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     9301 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     9361 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac
     9421 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     9481 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     9541 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     9601 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     9661 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     9721 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     9781 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     9841 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     9901 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     9961 agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa
    10021 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
    10081 ctcccaacga agataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
    10141 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
    10201 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
    10261 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
    10321 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
    10381 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    10441 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    10501 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
    10561 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
    10621 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga
    10681 gaccaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat
    10741 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
    10801 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
    10861 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
    10921 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    10981 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    11041 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    11101 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    11161 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    11221 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    11281 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    11341 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    11401 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    11461 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    11521 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
    11581 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    11641 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    11701 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    11761 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    11821 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    11881 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    11941 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    12001 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    12061 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    12121 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    12181 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    12241 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    12301 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    12361 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    12421 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    12481 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    12541 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    12601 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    12661 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    12721 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    12781 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    12841 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
    12901 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
    12961 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
    13021 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
    13081 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
    13141 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
    13201 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
    13261 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
    13321 gctg
//
