LOCUS       V025340                11162 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025340
VERSION     V025340
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5205
                     /label="Myo19(NM_025414)"
                     /note="Myo19(NM_025414)"
                     /gene="Myo19"
     CDS             5212..5277
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5321..5532
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5545..5813
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5842..5851
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5872..6534
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6604..6657
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6658..7257
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7258..7845
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7919..8152
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8224..8345
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8364..8499
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8537..8553)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8561..8577
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8585..8615)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8630..8651
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8939..9527)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9698..10558)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10559..10663)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11137..11153
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc tccagcaggt gaatggccac
     2341 agtttaggct ctgatgccga aggtagggcc tccctcaagg gagacctaag ggagttcctg
     2401 ggtggggaga tcccactgca ccagctggat gacctcacca aggtgaatcc cgtgacactg
     2461 gaaacagtcc tgaggtgcct gcaggcccga tacacagaag acatattcta taccaatgct
     2521 ggctgtacct tggtagctct gaaccccttt aagcacgtcc ctcaactcta tgcaccagaa
     2581 ctgatgcaag agtaccatgc tgcacctcag cctcagaaat tgaaacccca catcttcaca
     2641 gtgggtgaac aaacctatag aaatgtcaag agcctgattg agccggtcaa ccagtccatc
     2701 gtggtcagtg gagagagtgg agccggaaag acatggacat cccgatgcct gatgaagttc
     2761 tatgcggtgg tggctgcctc accgacttcc tgtgagaacc ataagattgc agagaggatt
     2821 gaacagcgga ttctgaactc caaccctgtg atggaagctt ttgggaacgc atgcacactg
     2881 aggaacagca acagcagccg cttcgggaag ttcattcagc tccaactcaa cagagcccag
     2941 cagatgacag gagctgcagt ccagacctac ctcctagaga aaacccgtgt ggcctgtcag
     3001 gcctccagtg agaggaactt tcacatcttc taccagattt gcaaaggagc caccaaggat
     3061 gaaaggctcc aatggcacct ccctgaggga actgccttct cctggctacc caatccagaa
     3121 agcagtttgg aagaggattg tttcgaggtg accagagagg ccatgctcca tttgggcatc
     3181 gacaccccca cccagaacaa catctttaag gtcctagctg gactgctgca ccttggcaat
     3241 gtccattttg ttgactcaga ggatgaagcc ctgccctgcc aagtgatgga tgataccaag
     3301 gtctctgtga ggacctcagc cttgctgctg cagctcccag agaaaatgct gctggagagt
     3361 atgcagattc gaaccatcaa ggcaggcaag cagcagcagg tgttccagaa gccctgctcc
     3421 cgagccgagt gtgacacacg cagagactgt ctggccaaac tgatctatgc acggctgttc
     3481 gactggctgg tgtctgtgat caacagcagc atctgtgcag actccaaatc atggactgcc
     3541 ttcatagggc tgctagatgt gtatgggttt gagtcatttc ctaataacag tttggaacag
     3601 ctgtgcatca actatgccaa tgagaagcta cagcagcact tcgtggctca ctacctcagg
     3661 gcccagcagg aggagtacga agttgagggc ctggagtggt catttgtcaa ctaccaggac
     3721 aaccagacct gcttagatct ccttgaaggg agccccatca gcatctgttc cctcataaat
     3781 gaggaatgcc gccttaaccg gccaagcagt gcagcacaac tacagacgcg catcgagagc
     3841 acgctggcag gacggccctg cctgggccat aacaagctca gccgggagcc cagcttcgtg
     3901 gttgtgcatt tcgcgggacc tgtacggtac cacacagccg gcctggtgga aaagaacaag
     3961 gaccctgttc cccctgagct gactgagctc ctgcagcaat cccaagaccc cctgctcacg
     4021 atgctgtttc ctgccaaccc tgaagagaag acccaggagg agctgtctgg ccagagccgg
     4081 gctcctgcat tgactgtggt gtccaagttc aaggcctcac tggaacagct cctgcaggtc
     4141 ctacataata caacacccca ctacattcgc tgcatcaagc ccaacagcca gagtcagcca
     4201 cagactttcc tccaagaaga ggtcctgaac cagttagagg cctgtggcct tgtggaaacc
     4261 attcacatca gtgctgctgg ctttcccatc cgggtctctc accagaactt catagaaaga
     4321 tataaactac tgagaaggct cggacctcgc atgtcctctg gcctcggggg cctggagcct
     4381 gccgaagggt cctctgagca gcctctgtgt gccaaggagg ccacactgca acctctacta
     4441 caggacattc tccatgctct gccagcttta attcagacag cagccactcc cagtgacccg
     4501 gctaagaaca cacaaatccc actgtactgt ggcaggacaa agatcttcat gactgactcc
     4561 atgctagagc ttctggaatg tgggcgtgcc cagatgctgg agcagtgtgc ccgttgcatc
     4621 cagtgtggct ggaggcgaca ccggctccaa aagcaggaga aacagaggcg ggctgccgtg
     4681 ctcatccagg ctgcttttag gtcctggtta actcggaaac acatcagaag gttacacata
     4741 gctgccacgg tcatcaagca cgcatggcat aagtggagaa tcagaatggc ctgtcttgcc
     4801 tctaaagaac tggatggtat ggaggagaaa cccatgcctc aggctcctgg taccctgcgg
     4861 tcctcgatgt ccccagcaca cactaggttc ctgggagcaa taatccatct ctggcccctg
     4921 ggactggtgc tagccaactc agctgatggc gtacgtggct ttcagaggaa actggtagcc
     4981 catgcctgcc ttcggcttcc ctcagacaga cccagcaaca aagtccagac accacaacag
     5041 gatcaggctg gtattacatc tatcagagca ctgccccagg gctcgataaa gtttcactgc
     5101 aggaagtctc cacttcagta tgctgacatc tgccctgacc cttcagcctc ctgtgttact
     5161 ggctttaatc agattctgct agaaagccac aggccagtcc aagtgggatc cgactacaaa
     5221 gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga tgacaagtag
     5281 cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc
     5341 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg
     5401 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     5461 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     5521 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct
     5581 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc
     5641 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt
     5701 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg
     5761 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg
     5821 tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg
     5881 gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc
     5941 ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc
     6001 ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc
     6061 acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc
     6121 aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc
     6181 tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac
     6241 agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc
     6301 atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc
     6361 tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc
     6421 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc
     6481 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc
     6541 cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct
     6601 cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg
     6661 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     6721 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     6781 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     6841 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     6901 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     6961 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     7021 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     7081 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     7141 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     7201 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat
     7261 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     7321 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     7381 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     7441 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     7501 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     7561 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     7621 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     7681 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     7741 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctccgc
     7801 cttcgccctc agacgagtcg gatctccctt tggccgcctc cccgcctggt acctttaaga
     7861 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     7921 gaagggctaa ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc
     7981 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     8041 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     8101 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     8161 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     8221 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     8281 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     8341 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc
     8401 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     8461 agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc
     8521 caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     8581 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     8641 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     8701 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     8761 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8821 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8881 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8941 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     9001 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     9061 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     9121 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     9181 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     9241 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     9301 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     9361 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     9421 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     9481 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9541 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9601 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     9661 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     9721 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9781 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9841 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9901 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9961 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    10021 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    10081 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    10141 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    10201 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    10261 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    10321 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    10381 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    10441 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    10501 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    10561 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    10621 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    10681 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
    10741 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
    10801 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
    10861 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
    10921 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
    10981 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
    11041 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
    11101 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc
    11161 tg
//
