LOCUS       V035497                10960 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035497
VERSION     V035497
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..5107
                     /label="Msh2(NM_008628)"
                     /note="Msh2(NM_008628)"
                     /gene="Msh2"
     promoter        5158..5369
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5382..5650
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5685..6392
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             6402..6455
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6456..7055
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7056..7643
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7717..7950
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8022..8143
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8162..8297
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8335..8351)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8359..8375
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8383..8413)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8428..8449
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8737..9325)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9496..10356)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10357..10461)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10935..10951
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatggcggt gcagcctaag gagacgctgc agttggaagg
     2341 cgcggccgag gcgggcttcg tgcgcttctt tgagggcatg ccggagaagc cgagcaccac
     2401 ggtgcgcctc ttcgaccgcg gcgactttta cacggcgcac ggagaggacg cgctgctggc
     2461 ggcccgcgag gtgttcaaga cccagggcgt gatcaagtac atggggccgg cagggagtaa
     2521 gaccctgcag agtgttgtgc ttagcaagat gaactttgag tctttcgtga aagatcttct
     2581 tctggttcgc cagtatcgag ttgaagttta taagaataaa gctggaaata aggcgtctaa
     2641 ggagaatgag tggtatctgg catttaaggc ttctcccggc aatctttctc agtttgaaga
     2701 catcctgttt ggtaacaatg acatgtcagc ttccgttggc gttatgggta ttaaaatggc
     2761 cgtggttgat ggtcaaagac atgttggagt tgggtatgtg gattccaccc agaggaagct
     2821 aggcttgtgt gagttccccg agaatgatca gttctccaat ctcgaggctc ttctgattca
     2881 gattggacca aaggaatgcg ttttaccagg aggagagact actggagaca tggggaaact
     2941 gaggcaggtt atccagagag gagggattct gatcacagaa agaaagagag ccgacttttc
     3001 cactaaagac atttatcagg atctcaaccg gttactgaaa ggcaaaaaag gagaacagat
     3061 aaatagtgct gccctaccag agatggagaa tcaggttgca gtttcatcac tatctgcagt
     3121 aatcaagttt ttagaactct tatcagacga ttcaaatttt gggcagtttg aactggccac
     3181 ttttgacttc agccagtaca tgaagttgga catggcagca gttagagccc tcaacctttt
     3241 ccagggttct gttgaagaca ccactggctc tcagtctctg gccgcattat tgaataaatg
     3301 caaaactgct caaggacaaa gattggttaa ccagtggatc aagcagccgc tcatggatag
     3361 gaacaggata gaggagaggt taaatttagt ggaagctttt gtcgaggatt cagaactgag
     3421 gcagagttta caggaggatt tgcttcgccg gtttccagac cttaaccggc ttgccaagaa
     3481 attccagaga caagcagcga atttacaaga ctgttaccga ctgtatcagg gtattaacca
     3541 gctccccagc gtcatccagg ctctggagaa atacgaagga agacaccagg cactgttgtt
     3601 ggcagttttt gtgactcctc ttattgatct tcgttctgat ttttcaaaat ttcaagaaat
     3661 gatagaaaca actttagata tggatcaggt ggaaaaccac gagttccttg taaaaccttc
     3721 atttgatcct aacctgagtg aactaagaga agtcatggat ggcctggaga agaagatgca
     3781 gtccacctta ataaatgcag cccgggggct cggattggat cctggcaaac agattaaatt
     3841 ggactccagt gcacagtttg gatattattt ccgtgtaacc tgcaaggaag agaaagtgct
     3901 tcgcaacaac aagaacttca gcacagtgga catccagaag aatggcgtga agtttaccaa
     3961 cagtgaattg tcctctttaa atgaagaata tactaagaac aaaggcgagt atgaagaggc
     4021 ccaggatgcc attgttaaag aaattgtcaa tatttcctca ggctacgtag agccaatgca
     4081 gacgctcaac gatgtgctgg ctcacttaga cgccattgtt agcttcgctc atgtgtcaaa
     4141 cgcagcaccc gttccttatg tacgaccagt catcttggag aaaggaaaag ggagaattat
     4201 attgaaagcc tccaggcatg cttgtgttga agttcaagat gaagttgcat ttattccaaa
     4261 tgacgtgcac tttgaaaaag ataaacagat gttccacatc attactggtc ccaatatggg
     4321 aggtaaatca acatacattc gtcagaccgg ggtgattgta ctcatggccc aaatcgggtg
     4381 ttttgtgccc tgtgagtcgg cagaagtgtc cattgtggat tgcatccttg ctcgagtcgg
     4441 ggctggtgac agtcaactga aaggcgtctc cacattcatg gctgaaatgc tggagactgc
     4501 ttccatcctc aggtcagcaa ccaaagactc cttaataatc attgatgagc tgggaagagg
     4561 aacctctacc tatgatggat ttgggttagc atgggctata tcagattaca ttgcaacgaa
     4621 gattggtgcc ttttgcatgt ttgccaccca ttttcatgaa cttactgctt tggccaacca
     4681 aataccaact gttaataatc tacatgtcac agcgctcact actgaggaga ccctaactat
     4741 gctttaccaa gtgaaaaaag gtgtctgtga tcagagtttc gggattcacg tggctgagct
     4801 cgctaacttc ccgaggcacg tgatagcgtg cgccaagcag aaggctctag agcttgaaga
     4861 atttcagaac attggaacct cgctgggatg tgacgaagcc gagccggctg caaagagacg
     4921 ctgcctggaa agagagcaag gtgagaaaat tattctggag ttcctgtcga aggtcaagca
     4981 ggtgcccttt actgccatgt cggaggagag catctccgcg aagctgaagc aactgaaagc
     5041 cgaggtggtc gcaaagaaca acagcttcgt aaacgagatc atttcacgga taaaggctcc
     5101 ggctccgtga ggatccgcgg ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg
     5161 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaacgg
     5221 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     5281 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     5341 ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt
     5401 cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc
     5461 ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt
     5521 cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca
     5581 cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc
     5641 gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggtg agcaagggcg
     5701 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
     5761 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     5821 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
     5881 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     5941 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     6001 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
     6061 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     6121 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
     6181 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
     6241 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
     6301 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
     6361 actccaccgg cggcatggac gagctgtaca agggatctcg cgagggcaga ggaagtcttc
     6421 taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag cccacggtgc
     6481 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     6541 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     6601 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     6661 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     6721 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     6781 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     6841 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     6901 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     6961 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     7021 gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat tacaaaattt
     7081 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     7141 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     7201 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     7261 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     7321 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     7381 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     7441 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     7501 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     7561 gcctgctgcc ggctctgcgg cctcttccgc gtctccgcct tcgccctcag acgagtcgga
     7621 tctccctttg gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg
     7681 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     7741 gaaaataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     7801 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7861 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7921 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     7981 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     8041 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     8101 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     8161 tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     8221 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     8281 aggctttttt ggaggcctag acttttgcag agacggccca aattcgtaat catggtcata
     8341 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8401 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     8461 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8521 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8581 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8641 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8701 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8761 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8821 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8881 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8941 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9001 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9061 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9121 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     9181 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9241 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9301 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9361 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9421 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9481 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9541 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9601 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9661 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9721 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9781 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9841 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9901 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9961 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10021 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10081 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10141 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    10201 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10261 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10321 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10381 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10441 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    10501 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
    10561 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
    10621 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    10681 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
    10741 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
    10801 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    10861 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    10921 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
