LOCUS       V032689                10882 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032689
VERSION     V032689
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4908
                     /label="Mlxipl(NM_021455)"
                     /note="Mlxipl(NM_021455)"
                     /gene="Mlxipl"
     CDS             4915..4980
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5024..5235
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5248..5516
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5545..5554
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5575..6237
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6307..6360
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6361..6960
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6961..7549
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7623..7856
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7928..8049
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8089..8224
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8257..8273)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8281..8297
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8305..8335)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8350..8371
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8659..9247)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9418..10278)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10279..10383)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10857..10873
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cgcgcgcgct ggcggatcta
     2341 tccgtgaact tgcaggtccc ccgggtcgtc cctagcccgg actcggactc ggatacggac
     2401 ttggaggatc cgagtccccg gcgcagcgcg ggtggcctgc atcgatcaca ggtcatccac
     2461 agcggacact tcatggtgtc ttcgccgcac agcgactcgc tgacccggcg acgcgaccag
     2521 gaggggcccg tggggctcgc cgacttcggg ccgcgcagca tcgatccgac actcacccac
     2581 ctcttcgagt gcttgagcct ggcttacagt ggcaagctgg tctctcccaa gtggaagaac
     2641 ttcaaaggcc tcaagttgct atgccgggac aagatccggc tgaacaacgc catctggaga
     2701 gcctggtaca ttcagtatgt gcaacggagg aagagcccag tgtgtggttt cgtgacccct
     2761 ctgcaggggt ctgaagcaga tgagcaccgg aaacctgagg ctgtcatcct ggagggtaat
     2821 tactggaagc ggcgcatcga ggtggtgatg cgcgaatacc acaagtggag aatctactac
     2881 aaaaagcggc tccgtaagtc cagcagggaa ggggatttcc tggctcccaa gcaggtggaa
     2941 ggtgggtggc cgccaccaga gcgatggtgc gaacagctct tctccagcgt ggtgcctgtg
     3001 ctgcttgggg gctccgagga ggagcctggg ggccggcagc ttctagacct ggactgcttc
     3061 ttgtccgata tctccgacac actcttcacc atgacacagc ccagtccttc gtccctgcag
     3121 ctgcccccag aagatgctta tgttggcaat gctgacatga tccagccaga cctgacgccg
     3181 ctgcagccca gcctagatga cttcatggag atatcagatt tcttcaccaa ttaccgcccc
     3241 cctcagacac ccacatcttc aaactacata gagtccccca gctttggccc catggctgac
     3301 tccctcttca gcagtgggat cctggcccca gagatgccat ctccagcctc gtcttcctcc
     3361 tcctcaggga tgacccctca ctcagggaat acacgcctac aggctcggaa cagctgctct
     3421 ggacccttgg atcccaaccc ctttctgagc tctgaattcc ttcttcctga agaccctaag
     3481 accaagatcc cacctgctcc tggaccgacc cctctccttc cattccccac ccctgtcaag
     3541 gtgcatggct tagagccctg caccccctct cccttcccca caatggctcc gcccccctct
     3601 ttgttgccag aagaatctct cctctctgcc aggttcccat tcacctcagc ccctcctgct
     3661 ccaggagtgt ctacgcttcc tgcacccaca acctttgtcc ccaccccaca gcccggccct
     3721 ggtcccgtcc ccttctctgt agaccatctg ccccatgggt acctggaacc cgtctttggg
     3781 cctcacttca ctgtgcctca gggtatgcaa cccaggtgca agccctcctc cccatcccct
     3841 gggggacaaa aagcaagtcc acccaccttg gcctctgcca ccgccagtcc cactgccact
     3901 gccactgcca gagacaacaa cccctgtctt acacagctgc tcagggcagc caagcctgag
     3961 caagcactgg agcctccgac tatgcccggt accctcctcc ggcctccaga gtccccgcag
     4021 gatacagttt ctgaaatccc ccgtgcccgt gcctttttcc ccccaatccc ggcccctaca
     4081 ccaccccggc cacctccagg cccagccaca ttggctcctc ccaggtccct ggttgtcccc
     4141 aaagcagagc ggctctcgcc tccagcctcc agcggcagtg agcggcgact atcaggggat
     4201 ctcaactcca tacaaccctc gggggcactg agtgtccacc tgtctccccc tcaaactgtc
     4261 ctaagccggg gtcgtgtaga caacaacaag atggagaacc gacgtatcac acacatctcc
     4321 gcggagcaga agaggcggtt caatattaag ctaggattcg acaccctgca cggacttgtc
     4381 agcacgctca gtgcccagcc cagcctcaag gtgagcaaag caaccacgct tcagaagaca
     4441 gcggagtaca tcctgatgct gcagcaggaa cgggcagcta tgcaggagga ggcgcagcag
     4501 ctgcgggatg aaatagagga gctcaacgct gccatcaact tgtgccagca gcagctaccg
     4561 gccaccgggg tgcccatcac acatcagcgc tttgaccaga tgcgggacat gtttgatgac
     4621 tatgtccgga cgcgcacgct gcacaactgg aagttctggg tgttcagcat cctcatccga
     4681 cctttatttg aatccttcaa tgggatggtg tctaccgcca gcttgcacag cctccgccag
     4741 acctcactgg cctggctgga acagtactgt tccctacctg ctctccggcc aactgtcctg
     4801 aattcccttc gccaactcag cacttccaca agcatcctga ctgaccccag ccttgtgcct
     4861 gagcaagcca cacgggcagt cacagagggc accctgggga gaccattagg atccgactac
     4921 aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag
     4981 tagcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg
     5041 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg
     5101 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     5161 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     5221 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc
     5281 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt
     5341 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt
     5401 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc
     5461 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag
     5521 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc
     5581 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc
     5641 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc
     5701 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc
     5761 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac
     5821 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac
     5881 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag
     5941 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac
     6001 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc
     6061 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc
     6121 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac
     6181 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga
     6241 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga
     6301 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct
     6361 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     6421 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     6481 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     6541 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     6601 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     6661 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     6721 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     6781 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     6841 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     6901 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     6961 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     7021 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     7081 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     7141 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     7201 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     7261 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     7321 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     7381 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     7441 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     7501 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggtaccttt
     7561 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     7621 actggaaggg ctaattcact cccaacgaag ataagatctg ctttttgctt gtactgggtc
     7681 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7741 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7801 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     7861 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     7921 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     7981 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     8041 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     8101 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     8161 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     8221 ggcctagact tttgcagaga ccaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa
     8281 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     8341 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8401 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8461 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8521 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8581 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8641 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8701 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8761 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8821 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8881 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8941 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9001 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9061 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     9121 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9181 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9241 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9301 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9361 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9421 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9481 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9541 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9601 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9661 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9721 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9781 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9841 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9901 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9961 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10021 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10081 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10141 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10201 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10261 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10321 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10381 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
    10441 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
    10501 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
    10561 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
    10621 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
    10681 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
    10741 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
    10801 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
    10861 aacgacggcc agtgccaagc tg
//
