LOCUS       V029717                14612 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029717
VERSION     V029717
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..3946
                     /EC_number=""
                     /codon_start=1
                     /experiment=""
                     /gene="luc"
                     /product="firefly luciferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
                     /label="luciferase"
     CDS             3977..8656
                     /label="Med1(NM_013634)"
                     /note="Med1(NM_013634)"
                     /gene="Med1"
     CDS             8663..8728
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        8771..8982
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             8995..9263
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      9292..9301
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             9322..9984
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             10054..10107
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             10108..10707
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    10708..11295
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             11369..11602
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    11674..11795
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      11814..11949
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(11987..12003)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    12011..12027
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(12035..12065)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    12080..12101
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(12389..12977)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(13148..14008)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(14009..14113)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     14587..14603
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta
     2341 tcctcttgag gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct
     2401 ggttcctgga acaattgctt ttacagatgc acatatcgag gtgaacatca cgtacgcgga
     2461 atacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa
     2521 tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc
     2581 gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct
     2641 caacagtatg aacatttcgc agcctaccgt agtgtttgtt tccaaaaagg ggttgcaaaa
     2701 aattttgaac gtgcaaaaaa aattaccaat aatccagaaa attattatca tggattctaa
     2761 aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg
     2821 ttttaatgaa tacgattttg taccagagtc ctttgatcgt gacaaaacaa ttgcactgat
     2881 aatgaactcc tctggatcta ctgggttacc taagggtgtg gcccttccgc atagaactgc
     2941 ctgcgtcaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac
     3001 tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta cactcggata
     3061 tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttttacg
     3121 atcccttcag gattacaaaa ttcaaagtgc gttgctagta ccaaccctat tttcattctt
     3181 cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg
     3241 gggcgcacct ctttcgaaag aagtcgggga agcggttgca aaacgcttcc atcttccagg
     3301 gatacgacaa ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg
     3361 ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga
     3421 tctggatacc gggaaaacgc tgggcgttaa tcagagaggc gaattatgtg tcagaggacc
     3481 tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga
     3541 tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact tcttcatagt
     3601 tgaccgcttg aagtctttaa ttaaatacaa aggataccag gtggcccccg ctgaattgga
     3661 gtcgatattg ttacaacacc ccaacatctt cgacgcgggc gtggcaggtc ttcccgacga
     3721 tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga
     3781 aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg
     3841 agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat
     3901 cagagagatc ctcataaagg ccaagaaggg cggaaagtcc aaattgggtg gaggcggatc
     3961 aggaggcggt ggatctatga gctccctcct ggaacggctc catgcaaaat ttaaccagaa
     4021 cagaccttgg agtgaaacca ttaagcttgt gcgtcaagta atggagaaga gggtcgtaat
     4081 gagttctgga gggcatcagc atttggtcag ctgtttggag acattgcaga aggctctcaa
     4141 agtaacatct ttgccagcaa tgactgatcg tttggaatct atagccagac agaatggact
     4201 gggctctcac ctcagtgcca gtggcactga gtgttacatc acgtcagata tgttctatgt
     4261 ggaagtgcag ttagatcctg caggacagct ttgtgatgtc aaagtggctc accatgggga
     4321 gaatcctgtg agctgtccag agcttgtaca gcagttaagg gaaaagaatt ttgaggaatt
     4381 ttccaagcat cttaagggtc ttgttaatct gtataatctc ccaggggaca acaaactgaa
     4441 gactaaaatg tatctggctc tccaatcctt agaacaggac ctttctaaaa tggctattat
     4501 gtactggaag gcaaccaacg ccgctccctt ggataagatt cttcatggaa gtgttggtta
     4561 tctcaccccg cggagtgggg gtcatttaat gaatatgaaa tactatgcct ctccatctga
     4621 cctgctggat gataagactg cctctcctat cattttgcat gaaaagaatg ttcctcggtc
     4681 tttgggaatg aatgcctcag tgacaattga aggaacctct gctatgtaca aactcccaat
     4741 tgccccatta attatggggt cacacccagc tgacaacaaa tggacccctt ctttctccgc
     4801 agtcactagt gccaacagtg ttgatcttcc tgcgtgtttc ttcttgaaat ttccccagcc
     4861 aattccagta tctaaagcat ttgttcagaa actgcaaaat tgcacaggaa tcccgttgtt
     4921 tgagactccg cccacttacc tgcccctgta tgaactcatc actcagtttg agctgtcaaa
     4981 ggatcctgac cctttacctt tgaatcacaa catgcgattt tacgctgctc ttccaggtca
     5041 gcagcactgc tattttctca ataaagatgc tcctcttcct gatggtcaga gcctgcaggg
     5101 aacactggtc agcaaaatca ccttccagca ccctggccga gttcctctta tcttgaatat
     5161 gatcagacac caagtggcct ataacactct aattggaagc tgtgtcaaaa gaactatttt
     5221 aaaagaagat tctcctgggc tcctccagtt tgaagtgtgt cctctctcag aatctcgctt
     5281 cagtgtatct tttcagcacc ctgtgaatga ctcccttgtg tgtgtggtga tggatgtgca
     5341 agactcaaca catgtgagct gtaaactcta caaggggctg tcagatgcac taatctgtac
     5401 agacgacttc attgccaaag ttgttcaaag atgtatgtcc attcctgtga cgatgagggc
     5461 tattcggagg aaggctgaaa ccatacaggc tgacacccca gcactgtctc tcattgcaga
     5521 gacagttgaa gacatggtga aaaagaacct gcccccggct agcagcccag ggtatggcat
     5581 gaccacaggc aacaacccaa tgagtggtac cactacacca accaacacct ttccgggggg
     5641 tcccattacc accttgttta atatgagcat gagcatcaaa gatcggcatg agtcggtggg
     5701 ccatggggag gacttcagca aggtgtctca gaacccaatt cttaccagtt tgttgcaaat
     5761 cacagggaac ggggggtcta ccattggctc gagtccgacc cctcctcatc acacgccgcc
     5821 acctgtctct tcgatggccg gcaacaccaa gaaccacccg atgctcatga accttcttaa
     5881 agataaccct gcccaggatt tctcaaccct ttatggaagc agccctttag aaaggcagaa
     5941 ctcctcttcc ggatcacccc ggatggaaat gtgctcgggg agcaacaagg ccaagaagaa
     6001 gaagtcgtca agagtcccac ctgacaaacc caagcaccag actgaagacg atttccagag
     6061 ggagctcttt tccatggatg tcgactcaca gaaccctatg tttgacgtca gcatgaccgc
     6121 tgacgcgctg gatacacctc atatcacccc agctccaagc cagtgtagca ctcccccagc
     6181 aacgtaccca cagccagtgt ctcaccccca gcccagtatt cagaggatgg tccgactgtc
     6241 cagttcagac agcattggcc cagatgtaac tgatattctt tcagatattg ccgaagaagc
     6301 ttcaaagctt cccagcacga gtgatgactg cccaccaatt ggcacccctg ttcgagattc
     6361 ctcaagttct gggcattctc agagtgccct ctttgattct gatgtctttc aaactaataa
     6421 taatgaaaat ccatacactg atccagctga ccttattgca gatgctgctg gaagccccaa
     6481 tagtgattct cctaccaatc attttttccc tgatggagta gatttcaatc ctgatttgtt
     6541 gaacagccaa agccaaagtg gttttggaga ggagtatttt gatgaaagta gtcagagtgg
     6601 ggataatgat gatttcaaag gatttgcatc tcaggcatta aatacattgg ggatgccaat
     6661 gcttggaggt gacaatgggg agccaaaatt taagggcagc agccaggctg acacggtgga
     6721 cttcagtatt atatcagtag ccggtaaggc tttgggtgct gcagatctga tggagcacca
     6781 cagtgggagt cagagtcctt tactgaccac tggagaatta gggaaagaaa aaactcaaaa
     6841 gagggtgaag gaaggcaacg gcacaggtgc tagcagtgga tcaggtccag ggtcagacag
     6901 caagccaggc aagcgcagcc gcactccctc caatgatggg aagagcaagg ataagcctcc
     6961 aaagcggaag aaggcagaca ctgaggggaa gtccccatct cacagttctt ctaatagacc
     7021 tttcacccca cctaccagca cgggtgggtc caaatcccca ggcagttcag gacgatctca
     7081 gacgccccca ggtgttgcca ccccgcccat tcccaagatt accattcaga ttcctaaagg
     7141 gacagtgatg gtgggcaagc cctcctctca cagtcagtac actagcagtg gttctgtgtc
     7201 ttcctctggc agcaaaagcc accatagtca ttcttcctcc tcctcttcct tagcttctgc
     7261 ttccacctca ggcaaggtga aaagcagtaa atctgaaggc tcatcaagtt ccaagctcag
     7321 tggcagtatg tatgctagcc aagggtcttc tggatccagc cagtccaaaa attcatctca
     7381 gactgggggg aagccaggct cctctcccat taccaaacat ggactgagca gtgggtccag
     7441 cagtaccaag atgaaacctc aaggcaagcc atcctccctt atgaaccctt ctataagtaa
     7501 gccaaacata tccccttccc attcaaggcc tcccggaggc tcagataagc ttgcctctcc
     7561 aatgaagcct gttcctggaa cccccccatc ctctaaagcc aagtccccta tcagttcagg
     7621 ttccagtggt tctcatgtgt caggaactag ttcaagctct ggtatgaagt catcttcagg
     7681 gtcagcatcc tcaggctcag tgtctcaaaa aacccctcca gcatctaatt cttgtacacc
     7741 atcttcctct tcgttttcct caagtggttc ttccatgtca tcctctcaga atcaacatgg
     7801 cagttccaaa gggaaatctc ccagtaggaa taagaagcct tccttgacag ctgtcataga
     7861 taaattgaag catggggtgg ttaccagtgg gcctgggggt gaggatccaa tagacagtca
     7921 gatgggcgca agcacaaatt cttctaacca tcccatgtcc tccaaacata acacgtcagg
     7981 aggggagttc cagagcaaac gtgagaaaag tgataaagac aaatccaagg tctctgcttc
     8041 tggggggtca gtggattcct ctaagaagac ttcagagtca aaaaatgtgg ggagcacggg
     8101 ggtggcaaaa atcattatca gcaagcacga cggaggctcc ccgagcatca aagccaaagt
     8161 gacgctacag aaacctggag aaagtggtgg agatgggctc aggccacaga tagcctcatc
     8221 aaagaactat ggctctccac ttatcagtgg ttccactcca aagcacgaac ggggttctcc
     8281 cagccacagt aagtcgccag catatacacc acagaatgtg gacagtgaaa gtgagtcagg
     8341 ctcctccata gcagagagat cctaccagaa cagtcccagc tcagaggatg gtatccgacc
     8401 acttccagag tacagcactg agaagcataa gaagcacaaa aaggaaaaga agaaagtcag
     8461 agacaaagac agagacaaga agaagtctca cagcatgaag ccagagaact ggtcgaaatc
     8521 ccccatttct tcagatccga cggcgtctgt gacaaataac cctatcttat ctgcagacag
     8581 gccttctagg ctcagccctg acttcatgat tggggaggaa gatgatgatc tcatggatgt
     8641 ggccctgatt ggcaatggat ccgactacaa agaccatgac ggtgattata aagatcatga
     8701 catcgactac aaggatgacg atgacaagta gcggccgcaa ggatctgcga tcgctccggt
     8761 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     8821 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     8881 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     8941 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct
     9001 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt
     9061 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt
     9121 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc
     9181 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt
     9241 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg
     9301 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg
     9361 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg
     9421 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac
     9481 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc
     9541 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc
     9601 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc
     9661 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc
     9721 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc
     9781 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg
     9841 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc
     9901 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc
     9961 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac
    10021 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc
    10081 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc
    10141 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
    10201 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
    10261 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
    10321 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
    10381 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
    10441 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
    10501 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
    10561 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
    10621 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
    10681 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga
    10741 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
    10801 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
    10861 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
    10921 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
    10981 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
    11041 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
    11101 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
    11161 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
    11221 ccggctctgc ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg gatctccctt
    11281 tggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
    11341 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa
    11401 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
    11461 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
    11521 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
    11581 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
    11641 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
    11701 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    11761 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
    11821 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
    11881 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
    11941 ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc
    12001 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
    12061 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
    12121 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
    12181 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
    12241 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
    12301 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
    12361 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
    12421 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
    12481 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    12541 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
    12601 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    12661 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    12721 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    12781 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
    12841 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    12901 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    12961 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    13021 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    13081 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    13141 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    13201 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    13261 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    13321 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    13381 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    13441 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    13501 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    13561 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    13621 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    13681 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    13741 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    13801 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    13861 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    13921 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    13981 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    14041 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    14101 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
    14161 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
    14221 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
    14281 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
    14341 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
    14401 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
    14461 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
    14521 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
    14581 gacgttgtaa aacgacggcc agtgccaagc tg
//
