LOCUS       V041823                10662 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041823
VERSION     V041823
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2279..2288
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             2288..2359
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags"
                     /transl_table=1
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
                     /label="3xFLAG"
     CDS             2366..4771
                     /label="Map3k20(NM_023057)"
                     /note="Map3k20(NM_023057)"
                     /gene="Map3k20"
     promoter        4821..5032
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5045..5313
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5342..5351
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5372..6034
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6104..6157
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6158..6757
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6758..7345
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7419..7652
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7724..7845
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7864..7999
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8037..8053)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8061..8077
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8085..8115)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8130..8151
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8439..9027)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9198..10058)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10059..10163)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10637..10653
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 caccatggac tacaaggatg acgatgacaa ggattacaaa gacgacgatg ataaggacta
     2341 taaggatgat gacgacaaag aattcatgtc gtctctcgga gcctcctttg tgcaaattaa
     2401 gttcgacgac ttgcagtttt ttgaaaactg cggtggagga agttttggga gtgtgtatcg
     2461 agccaaatgg atatcacagg acaaggaggt ggctgtaaag aagttactca aaatagagaa
     2521 agaggcagaa atcctgagcg tcctcagtca cagaaacatc atccagtttt atggagtgat
     2581 tctggaacct cccaactatg gcatcgtcac agaatatgct tccctgggct ccctgtatga
     2641 ttacattaac agcaacagga gtgaagagat ggacatggaa cacatcatga cctgggccac
     2701 tgacgtagcc aaagggatgc attacttaca catggaagct cctgtcaagg tgatccacag
     2761 agacctcaag tcaagaaacg ttgttatcgc tgctgacgga gtgctgaaga tctgtgactt
     2821 cggtgcctcg cggttccata accacacaac acacatgtcc ttggttggaa ctttcccatg
     2881 gatggctcca gaagttatcc agagtctccc tgtgtctgaa acctgtgaca cgtattccta
     2941 tggtgtggtt ctctgggaga tgctaacaag ggaggtcccc tttaaaggtt tggaaggatt
     3001 acaagtagct tggcttgtag tggaaaaaaa cgagagatta accattccaa gcagctgccc
     3061 cagaagcttt gctgaactgc tacaccagtg ttgggaggct gatgccaaga aacggccatc
     3121 gttcaagcaa atcatttcaa tcctggagtc tatgtcaaat gacacgaacc ttcctgacca
     3181 gtgtaactca tttttacaca acaaggcgga gtggaggtgt gaaattgagg caacccttga
     3241 gcgactgaag aaactagagc gagatctcag ctttaaagag caggagctca aagaacggga
     3301 gagacgtctc aagatgtggg agcagaagct gacggagcaa tccaacaccc cgctgctgcc
     3361 ttcctttgag attggtgcgt ggactgaaga cgatgtgtat ttttgggttc agcagctcgt
     3421 cagaaaaggc gagtcttcag tagagatgag tggatacgca agtttgttta aggagaacaa
     3481 catcacaggc aagcggttgc tgcttctgga agaggaagat ctgaaagaca tgggcatcgt
     3541 ctccaaggga cacatcattc actttaagtc agctattgag aagctaaccc acgattatct
     3601 gaacctgttt cacttcccac cactgattaa ggattcagga ggggaacctg aagaaaatga
     3661 ggaaaaaata gtgaaccttg aacttgtttt tggttttcac ttgaagccag gaactggccc
     3721 acaggattgc aaatggaaaa tgtatatgga gatggatggg gacgaagttg caatcacgta
     3781 cataaaagat gtgactttca acacgagcct ccctgatgca gagattttaa agatgacaaa
     3841 gccaccattt gtaatggaga agtggatcgt gggaatagcg gaggatcaga ctgtggagtg
     3901 cacggtcacc tatgagaatg atgtcagaac accaaaactc actaagcacg tgcactccat
     3961 acagtgggac agaacgaagc ctcaggatga ggtgaaggcg gtccagcttg ccattcagac
     4021 tctgttctcc agctcagagg gcaaccctgg cagcagatcc gactccagtg cggactgcca
     4081 atggttagac actctgcgca tgcggcagat tgcatcccac acttctctac agcgttccca
     4141 aagcaaccct atcttggggt ctcctttctt cccttacttt gccaaccagg attcctatgc
     4201 tgctgcggtg aggaggaccc agactccggt gaagtaccag cagattacac caagcatcaa
     4261 cccttcccgg agctcctccc ccacgcagta tgggctgtcc agaaacttct cctccctgaa
     4321 cctcagctcc agggacagcg gcttctccag cctcaatgac agctcctccg agaggggccg
     4381 ctactcagac cgaagcagaa acaagtacta ccgtggtagt gtgtccctca attcttcccc
     4441 gaaaggaaga tatggtggga aaagtcagca ttccacacct tcaagagaaa gatattctgg
     4501 aaagttctac aggctgcccc agtcagcgct gaacactcat cagtcccctg acttcaagag
     4561 gagcccaaat gaccatgacc gccgtgtgcc caggaccata cccgggatgc ctctgcaccc
     4621 tgagactgcc tccaaagcag gggaagagga gagcagggtg agcgaagggg gctggactaa
     4681 agtggaatat cggaagaaga cacacaggca actttcggcc aagactagca aggagagaac
     4741 ccgtggcaac taccgtgggc ggcggaattt ttaggatccg cggccgcgaa ggatctgcga
     4801 tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     4861 gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag
     4921 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     4981 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct
     5041 tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg
     5101 ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt
     5161 ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct
     5221 acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt
     5281 tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga
     5341 cgccaccatg gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac
     5401 cggcaccctg aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca
     5461 gggccgcatg accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct
     5521 gctgagccac gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga
     5581 gaaccccttc ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta
     5641 cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat
     5701 cggcgacttc aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa
     5761 gatcatccgc agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt
     5821 gggcagcttc gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga
     5881 cagccacatg cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat
     5941 gttcgccttc cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta
     6001 ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc
     6061 tgccgtggac ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct
     6121 tctaacatgc ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt
     6181 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     6241 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     6301 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     6361 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     6421 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     6481 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     6541 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     6601 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     6661 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     6721 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat
     6781 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     6841 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     6901 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     6961 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     7021 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     7081 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     7141 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     7201 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     7261 cggcctgctg ccggctctgc ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg
     7321 gatctccctt tggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc
     7381 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     7441 acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     7501 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7561 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7621 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     7681 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     7741 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7801 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     7861 gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     7921 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     7981 ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca
     8041 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     8101 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     8161 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     8221 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     8281 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     8341 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8401 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8461 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8521 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8581 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     8641 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     8701 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     8761 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8821 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     8881 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8941 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9001 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9061 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9121 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9181 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9241 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9301 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9361 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9421 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9481 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9541 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9601 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     9661 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     9721 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9781 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     9841 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9901 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9961 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10021 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10081 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    10141 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
    10201 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
    10261 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
    10321 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
    10381 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
    10441 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
    10501 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
    10561 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
    10621 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//
