LOCUS       V031774                11614 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031774
VERSION     V031774
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5640
                     /label="MYO1E(NM_004998)"
                     /note="MYO1E(NM_004998)"
                     /gene="MYO1E"
     CDS             5647..5712
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5756..5967
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5980..6248
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6277..6286
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6307..6969
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7039..7092
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7093..7692
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7693..8281
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8355..8588
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8660..8781
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8821..8956
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8989..9005)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9013..9029
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9037..9067)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9082..9103
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9391..9979)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10150..11010)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11011..11115)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11589..11605
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg gaagcaaagg tgtctaccag
     2341 taccactggc aaagccacaa tgtcaagcac agtggtgtgg acgacatggt gctactgtcc
     2401 aagatcacag agaactccat cgtggagaat ctgaagaaga gatacatgga tgactacatt
     2461 tttacatata taggatctgt attaatctca gtcaaccctt tcaagcagat gccatatttt
     2521 ggggaaaagg aaattgaaat gtaccaagga gcggcacagt atgaaaaccc accacatatc
     2581 tatgcccttg cagataatat gtacagaaac atgatcattg acagagagaa ccagtgcgtc
     2641 attatcagtg gtgaaagtgg tgctggaaaa acagtggctg ccaaatatat catgagctac
     2701 atctccagag tgtctggagg agggaccaaa gtccagcacg tgaaggacat tatcctgcag
     2761 tccaacccgc tgctggaggc cttcgggaac gccaagaccg tccggaacaa caactccagc
     2821 cgatttggaa aatactttga aatccagttc agtccaggtg gggaaccaga tggtggaaag
     2881 atctccaact tccttctgga aaaatctagg gtggtgatga ggaacccagg agagcggagt
     2941 tttcacatat tttaccagct catcgagggc gcctctgcag agcagaaaca cagccttggc
     3001 atcaccagca tggactatta ttactacctg agcctctcgg gctcatacaa ggttgatgac
     3061 attgacgaca ggcgggagtt tcaggaaact ctgcacgcca tgaatgtgat tgggatcttt
     3121 gcagaagagc aaacgctggt gttgcagata gtggcgggta ttctccacct gggaaacatc
     3181 agcttcaaag aagttggcaa ctacgcggct gtggagagtg aagagttttt agcttttcct
     3241 gcatatctgc tagggataaa ccaggaccgg ttgaaagaaa agctaacaag ccggcagatg
     3301 gatagcaagt ggggaggcaa atccgaatcc atccacgtga ccctcaacgt agagcaggcc
     3361 tgttacaccc gggatgcgct cgccaaggcc ctgcacgccc gggtctttga tttcttggta
     3421 gattccatca ataaagccat ggagaaagac catgaagaat acaacattgg cgtcctagac
     3481 atctatggct ttgaaatatt ccagaaaaat ggctttgaac agttttgtat caattttgtt
     3541 aatgaaaaac tgcagcagat ttttattgaa ctgacattaa aggcagaaca ggaagaatat
     3601 gttcaagagg gaataagatg gacacccatt gagtacttta ataataaaat cgtatgtgac
     3661 ctcatagaga acaaagtgaa ccctcctggc atcatgagca tcctggatga cgtgtgcgcc
     3721 acgatgcatg cggtgggtga gggggcagat cagacgctgc tccagaaact tcagatgcag
     3781 attgggagtc atgagcactt caacagttgg aaccaaggct tcatcattca tcattatgct
     3841 gggaaggtat cctatgacat ggatggcttt tgtgaaagga accgggatgt gctttttatg
     3901 gatctcatcg agcttatgca gagcagcgag ctgcctttca taaagtcttt atttccggaa
     3961 aatctgcagg ctgacaagaa agggcgccca actactgccg gaagcaaaat aaagaaacaa
     4021 gccaatgacc ttgtgagcac cctgatgaaa tgtacgcccc actacattcg ctgcatcaag
     4081 ccaaacgaaa ccaagaagcc cagagactgg gaggaaagca gggtaaagca tcaagtcgaa
     4141 tatttgggtc tgaaagagaa cattcgagtg agaagagctg gctatgccta tcggcgcatc
     4201 ttccaaaaat tcctacagag gtatgccatt ctgaccaaag ccacctggcc ttcttggcag
     4261 ggagaggaga agcaaggcgt cctgcacctg ctgcagtcgg tcaacatgga cagcgaccag
     4321 ttccagctgg ggaggagtaa agtgttcatc aaagcccccg agtctctatt tcttttagaa
     4381 gagatgagag agagaaagta tgatgggtat gctcgagtga tacagaaatc atggaggaaa
     4441 ttcgtggccc ggaagaaata cgttcaaatg agagaagaag cctcagacct cttattgaac
     4501 aagaaggaga gaaggagaaa cagtattaac aggaacttta taggggatta tattgggatg
     4561 gaagagcacc cagaactcca gcagttcgtg ggcaagaggg agaagattga tttcgcagac
     4621 acagtcacca agtatgacag gaggttcaag ggtgtaaagc gagacctgct ccttacccca
     4681 aagtgcttgt acttaatcgg acgagaaaaa gtcaaacagg gcccagacaa gggcctggtg
     4741 aaagaagtcc tgaagcggaa aatcgagata gaacggatct tgtctgtgtc cctcagtact
     4801 atgcaggatg acatttttat tctccatgag caagagtatg acagtttgct tgaatctgtc
     4861 ttcaaaactg aattcctaag cctcttagca aagcgttacg aggagaagac ccagaagcaa
     4921 ctacctctga aattcagcaa tacgcttgaa ctgaagttga aaaaggaaaa ctggggcccc
     4981 tggagtgcag ggggctcccg gcaagtgcag ttccaccaag ggtttgggga cctggctgtc
     5041 ctcaagccca gtaacaaagt gctgcaggtc agcatcggac ctggactgcc caagaactcc
     5101 cgtcctacca gaaggaacac tacccaaaat acaggttatt ccagtgggac tcaaaatgcc
     5161 aactacccag tgagagctgc ccctcctccc ccaggatacc atcagaacgg agtcatcaga
     5221 aaccagtatg tgccatatcc ccatgctcct ggaagccaga ggtccaatca gaaaagcctg
     5281 tacacctcca tggcccgccc gcccttgcct cggcagcagt ctaccagttc agaccgagtg
     5341 tcacagacgc cagagagcct ggatttcctc aaggtcccgg accagggagc tgcaggggtc
     5401 aggagacaaa caaccagtcg gcctccccca gcagggggca gacccaagcc ccagcccaag
     5461 cccaagcctc aggtgccaca gtgcaaggct ttgtatgcct atgacgctca ggacacagac
     5521 gaactcagct ttaatgccaa tgacattatt gatattatca aagaagatcc ttctggctgg
     5581 tggacgggtc gactacgagg caagcagggc ctgttcccca acaactatgt gaccaagatc
     5641 ggatccgact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
     5701 gacgatgaca agtagcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca
     5761 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt
     5821 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     5881 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     5941 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca
     6001 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct
     6061 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
     6121 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
     6181 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
     6241 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc
     6301 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc
     6361 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag
     6421 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc
     6481 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac
     6541 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg
     6601 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc
     6661 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg
     6721 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc
     6781 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc
     6841 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag
     6901 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc
     6961 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc
     7021 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag
     7081 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     7141 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     7201 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     7261 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     7321 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     7381 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     7441 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     7501 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     7561 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     7621 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     7681 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     7741 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     7801 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     7861 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     7921 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     7981 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     8041 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     8101 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     8161 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     8221 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     8281 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     8341 agaaaagggg ggactggaag ggctaattca ctcccaacga agataagatc tgctttttgc
     8401 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     8461 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     8521 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     8581 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     8641 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     8701 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     8761 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     8821 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8881 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     8941 ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt catagctgtt
     9001 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     9061 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     9121 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     9181 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     9241 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     9301 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     9361 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     9421 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     9481 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     9541 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     9601 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     9661 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     9721 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     9781 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     9841 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     9901 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     9961 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    10021 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    10081 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    10141 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    10201 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    10261 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    10321 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    10381 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    10441 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    10501 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    10561 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    10621 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    10681 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    10741 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    10801 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    10861 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    10921 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    10981 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    11041 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    11101 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    11161 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
    11221 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
    11281 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
    11341 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
    11401 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
    11461 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
    11521 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
    11581 acgacgttgt aaaacgacgg ccagtgccaa gctg
//
