LOCUS       V026480                13188 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026480
VERSION     V026480
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2301..8042
                     /label="MYLK(NM_053025)"
                     /note="MYLK(NM_053025)"
                     /gene="MYLK"
     CDS             8049..8114
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        8158..8369
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             8382..8650
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             8677..9276
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    9283..9871
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9945..10178
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    10250..10371
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      10390..10525
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10563..10579)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10587..10603
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10611..10641)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10656..10677
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10965..11553)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11724..12584)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12585..12689)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     13163..13179
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagattcta
     2281 gagctagcga attcgccacc atgggggatg tgaagctggt tgcctcgtca cacatttcca
     2341 aaacctccct cagtgtggat ccctcaagag ttgactccat gcccctgaca gaggcccctg
     2401 ctttcatttt gccccctcgg aacctctgca tcaaagaagg agccaccgcc aagttcgaag
     2461 ggcgggtccg gggttaccca gagccccagg tgacatggca cagaaacggg caacccatca
     2521 ccagcggggg ccgcttcctg ctggattgcg gcatccgggg gactttcagc cttgtgattc
     2581 atgctgtcca tgaggaggac aggggaaagt atacctgtga agccaccaat ggcagtggtg
     2641 ctcgccaggt gacagtggag ttgacagtag aaggaagttt tgcgaagcag cttggtcagc
     2701 ctgttgtttc caaaacctta ggggatagat tttcagctcc agcagtggag acccgtccta
     2761 gcatctgggg ggagtgccca ccaaagtttg ctaccaagct gggccgagtt gtggtcaaag
     2821 aaggacagat gggacgattc tcctgcaaga tcactggccg gccccaaccg caggtcacct
     2881 ggctcaaggg aaatgttcca ctgcagccga gtgcccgtgt gtctgtgtct gagaagaacg
     2941 gcatgcaggt tctggaaatc catggagtca accaagatga cgtgggagtg tacacgtgcc
     3001 tggtggtgaa cgggtcgggg aaggcctcga tgtcagctga actttccatc caaggtttgg
     3061 acagtgccaa taggtcattt gtgagagaaa caaaagccac caattcagat gtcaggaaag
     3121 aggtgaccaa tgtaatctca aaggagtcga agctggacag tctggaggct gcagccaaaa
     3181 gcaagaactg ctccagcccc cagagaggtg gctccccacc ctgggctgca aacagccagc
     3241 ctcagccccc aagggagtcc aagctggagt catgcaagga ctcgcccaga acggccccgc
     3301 agaccccggt ccttcagaag acttccagct ccatcaccct gcaggccgca agagttcagc
     3361 cggaaccaag agcaccaggc ctgggggtcc tatcaccttc tggagaagag aggaagaggc
     3421 cagctcctcc ccgtccagcc accttcccca ccaggcagcc tggcctgggg agccaagatg
     3481 ttgtgagcaa ggctgctaac aggagaatcc ccatggaggg ccagagggat tcagcattcc
     3541 ccaaatttga gagcaagccc caaagccagg aggtcaagga aaatcaaact gtcaagttca
     3601 gatgtgaagt ttccgggatt ccaaagcctg aagtggcctg gttcctggaa ggcacccccg
     3661 tgaggagaca ggaaggcagc attgaggttt atgaagatgc tggctcccat tacctctgcc
     3721 tgctgaaagc ccggaccagg gacagtggga catacagctg cactgcttcc aacgcccaag
     3781 gccagctgtc ctgtagctgg accctccaag tggaaaggct tgccgtgatg gaggtggccc
     3841 cctccttctc cagtgtcctg aaggactgcg ctgttattga gggccaggat tttgtgctgc
     3901 agtgctccgt acgggggacc ccagtgcccc ggatcacttg gctgctgaat gggcagccca
     3961 tccagtacgc tcgctccacc tgcgaggccg gcgtggctga gctccacatc caggatgccc
     4021 tgccggagga ccatggcacc tacacctgcc tagctgagaa tgccttgggg caggtgtcct
     4081 gcagcgcctg ggtcaccgtc catgaaaaga agagtagcag gaagagtgag taccttctgc
     4141 ctgtggctcc cagcaagccc actgcaccca tcttcctgca gggcctctct gatctcaaag
     4201 tcatggatgg aagccaggtc actatgactg tccaagtgtc agggaatcca ccccctgaag
     4261 tcatctggct gcacaatggg aatgagatcc aagagtcaga ggacttccac tttgaacaga
     4321 gaggaactca gcacagcctt tgtatccagg aagtgttccc ggaggacacg ggcacgtaca
     4381 cctgcgaggc ctggaacagc gctggagagg tccgcaccca ggccgtgctc acggtacaag
     4441 agcctcacga tggcacccag ccctggttca tcagtaagcc tcgctcagtg acagcctccc
     4501 tgggccagag tgtcctcatc tcctgcgcca tagctggtga cccctttcct accgtgcact
     4561 ggctcagaga tggcaaagcc ctctgcaaag acactggcca cttcgaggtg cttcagaatg
     4621 aggacgtgtt caccctggtt ctaaagaagg tgcagccctg gcatgccggc cagtatgaga
     4681 tcctgctcaa gaaccgggtt ggcgaatgca gttgccaggt gtcactgatg ctacagaaca
     4741 gctctgccag agcccttcca cgggggaggg agcctgccag ctgcgaggac ctctgtggtg
     4801 gaggagttgg tgctgatggt ggtggtagtg accgctatgg gtccctgagg cctggctggc
     4861 cagcaagagg gcagggttgg ctagaggagg aagacggcga ggacgtgcga ggggtgctga
     4921 agaggcgcgt ggagacgagg cagcacactg aggaggcgat ccgccagcag gaggtggagc
     4981 agctggactt ccgagacctc ctggggaaga aggtgagtac aaagacccta tcggaagacg
     5041 acctgaagga gatcccagcc gagcagatgg atttccgtgc caacctgcag cggcaagtga
     5101 agccaaagac tgtgtctgag gaagagagga aggtgcacag cccccagcag gtcgattttc
     5161 gctctgtcct ggccaagaag gggacttcca agacccccgt gcctgagaag gtgccaccgc
     5221 caaaacctgc caccccggat tttcgctcag tgctgggtgg caagaagaaa ttaccagcag
     5281 agaatggcag cagcagtgcc gagaccctga atgccaaggc agtggagagt tccaagcccc
     5341 tgagcaatgc acagccttca gggcccttga aacccgtggg caacgccaag cctgctgaga
     5401 ccctgaagcc aatgggcaac gccaagcctg ccgagaccct gaagcccatg ggcaatgcca
     5461 agcctgatga gaacctgaaa tccgctagca aagaagaact caagaaagac gttaagaatg
     5521 atgtgaactg caagagaggc catgcaggga ccacagataa tgaaaagaga tcagagagcc
     5581 aggggacagc cccagccttc aagcagaagc tgcaagatgt tcatgtggca gagggcaaga
     5641 agctgctgct ccagtgccag gtgtcttctg accccccagc caccatcatc tggacgctga
     5701 acggaaagac cctcaagacc accaagttca tcatcctctc ccaggaaggc tcactctgct
     5761 ccgtctccat cgagaaggca ctgcctgagg acagaggctt atacaagtgt gtagccaaga
     5821 atgacgctgg ccaggcggag tgctcctgcc aagtcaccgt ggatgatgct ccagccagtg
     5881 agaacaccaa ggccccagag atgaaatccc ggaggcccaa gagctctctt cctcccgtgc
     5941 taggaactga gagtgatgcg actgtgaaaa agaaacctgc ccccaagaca cctccgaagg
     6001 cagcaatgcc ccctcagatc atccagttcc ctgaggacca gaaggtacgc gcaggagagt
     6061 cagtggagct gtttggcaaa gtgacaggca ctcagcccat cacctgtacc tggatgaagt
     6121 tccgaaagca gatccaggaa agcgagcaca tgaaggtgga gaacagcgag aatggcagca
     6181 agctcaccat cctggccgcg cgccaggagc actgcggctg ctacacactg ctggtggaga
     6241 acaagctggg cagcaggcag gcccaggtca acctcactgt cgtggataag ccagaccccc
     6301 cagctggcac accttgtgcc tctgacattc ggagctcctc actgaccctg tcctggtatg
     6361 gctcctcata tgatgggggc agtgctgtac agtcctacag catcgagatc tgggactcag
     6421 ccaacaagac gtggaaggaa ctagccacat gccgcagcac ctctttcaac gtccaggacc
     6481 tgctgcctga ccacgaatat aagttccgtg tacgtgcaat caacgtgtat ggaaccagtg
     6541 agccaagcca ggagtctgaa ctcacaacgg taggagagaa acctgaagag ccgaaggatg
     6601 aagtggaggt gtcagatgat gatgagaagg agcccgaggt tgattaccgg acagtgacaa
     6661 tcaatactga acaaaaagta tctgacttct acgacattga ggagagatta ggatctggga
     6721 aatttggaca ggtctttcga cttgtagaaa agaaaactcg aaaagtctgg gcagggaagt
     6781 tcttcaaggc atattcagca aaagagaaag agaatatccg gcaggagatt agcatcatga
     6841 actgcctcca ccaccctaag ctggtccagt gtgtggatgc ctttgaagaa aaggccaaca
     6901 tcgtcatggt cctggagatc gtgtcaggag gggagctgtt tgagcgcatc attgacgagg
     6961 actttgagct gacggagcgt gagtgcatca agtacatgcg gcagatctcg gagggagtgg
     7021 agtacatcca caagcagggc atcgtgcacc tggacctcaa gccggagaac atcatgtgtg
     7081 tcaacaagac gggcaccagg atcaagctca tcgactttgg tctggccagg aggctggaga
     7141 atgcggggtc tctgaaggtc ctctttggca ccccagaatt tgtggctcct gaagtgatca
     7201 actatgagcc catcggctac gccacagaca tgtggagcat cggggtcatc tgctacatcc
     7261 tagtcagtgg cctttccccc ttcatgggag acaacgataa cgaaaccttg gccaacgtta
     7321 cctcagccac ctgggacttc gacgacgagg cattcgatga gatctccgac gatgccaagg
     7381 atttcatcag caatctgctg aagaaagata tgaaaaaccg cctggactgc acgcagtgcc
     7441 ttcagcatcc atggctaatg aaagatacca agaacatgga ggccaagaaa ctctccaagg
     7501 accggatgaa gaagtacatg gcaagaagga aatggcagaa aacgggcaat gctgtgagag
     7561 ccattggaag actgtcctct atggcaatga tctcagggct cagtggcagg aaatcctcaa
     7621 cagggtcacc aaccagcccg ctcaatgcag aaaaactaga atctgaagaa gatgtgtccc
     7681 aagctttcct tgaggctgtt gctgaggaaa agcctcatgt aaaaccctat ttctctaaga
     7741 ccattcgcga tttagaagtt gtggagggaa gtgctgctag atttgactgc aagattgaag
     7801 gatacccaga ccccgaggtt gtctggttca aagatgacca gtcaatcagg gagtcccgcc
     7861 acttccagat agactacgat gaggacggga actgctcttt aattattagt gatgtttgcg
     7921 gggatgacga tgccaagtac acctgcaagg ctgtcaacag tcttggagaa gccacctgca
     7981 cagcagagct cattgtggaa acgatggagg aaggtgaagg ggaaggggaa gaggaagaag
     8041 agggatccga ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg
     8101 atgacgatga caagtagcgg ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg
     8161 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaacgg
     8221 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     8281 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     8341 ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt
     8401 cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc
     8461 ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt
     8521 cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca
     8581 cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc
     8641 gttacagatc caagctgtga ccggcgccta cgctagatga ccgagtacaa gcccacggtg
     8701 cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc
     8761 gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag
     8821 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     8881 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     8941 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     9001 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     9061 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     9121 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     9181 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     9241 cgcacctggt gcatgacccg caagcccggt gcctgagtcg acaatcaacc tctggattac
     9301 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     9361 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     9421 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     9481 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     9541 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     9601 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     9661 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     9721 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     9781 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     9841 agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa
     9901 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     9961 ctcccaacga aaataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
    10021 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
    10081 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
    10141 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
    10201 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
    10261 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    10321 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    10381 gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
    10441 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
    10501 tagtgaggag gcttttttgg aggcctagac ttttgcagag acggcccaaa ttcgtaatca
    10561 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
    10621 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
    10681 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
    10741 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
    10801 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    10861 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    10921 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    10981 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    11041 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    11101 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    11161 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    11221 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    11281 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    11341 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    11401 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    11461 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    11521 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    11581 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    11641 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    11701 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    11761 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    11821 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    11881 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    11941 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    12001 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    12061 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    12121 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    12181 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    12241 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    12301 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    12361 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    12421 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    12481 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    12541 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    12601 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    12661 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    12721 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
    12781 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
    12841 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
    12901 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
    12961 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
    13021 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
    13081 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
    13141 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg
//
