LOCUS       V035416                11945 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035416
VERSION     V035416
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..6123
                     /label="MYBPC3(NM_000256)"
                     /note="MYBPC3(NM_000256)"
                     /gene="MYBPC3"
     CDS             6160..6873
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of red fluorescent protein
                     from Entacmaea quadricolor&nbsp;(Merzlyak et al., 2007)"
                     /transl_table=1
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN*"
                     /label="TagRFP"
     promoter        6915..7126
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7139..7407
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             7434..8033
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8040..8628
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8702..8935
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9007..9128
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9147..9282
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9320..9336)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9344..9360
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9368..9398)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9413..9434
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9722..10310)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10481..11341)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11342..11446)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11920..11936
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgcctgag ccggggaaga agccagtctc agcttttagc
     2341 aagaagccac ggtcagtgga agtggccgca ggcagccctg ccgtgttcga ggccgagaca
     2401 gagcgggcag gagtgaaggt gcgctggcag cgcggaggca gtgacatcag cgccagcaac
     2461 aagtacggcc tggccacaga gggcacacgg catacgctga cagtgcggga agtgggccct
     2521 gccgaccagg gatcttacgc agtcattgct ggctcctcca aggtcaagtt cgacctcaag
     2581 gtcatagagg cagagaaggc agagcccatg ctggcccctg cccctgcccc tgctgaggcc
     2641 actggagccc ctggagaagc cccggcccca gccgctgagc tgggagaaag tgccccaagt
     2701 cccaaagggt caagctcagc agctctcaat ggtcctaccc ctggagcccc cgatgacccc
     2761 attggcctct tcgtgatgcg gccacaggat ggcgaggtga ccgtgggtgg cagcatcacc
     2821 ttctcagccc gcgtggccgg cgccagcctc ctgaagccgc ctgtggtcaa gtggttcaag
     2881 ggcaaatggg tggacctgag cagcaaggtg ggccagcacc tgcagctgca cgacagctac
     2941 gaccgcgcca gcaaggtcta tctgttcgag ctgcacatca ccgatgccca gcctgccttc
     3001 actggcagct accgctgtga ggtgtccacc aaggacaaat ttgactgctc caacttcaat
     3061 ctcactgtcc acgaggccat gggcaccgga gacctggacc tcctatcagc cttccgccgc
     3121 acgagcctgg ctggaggtgg tcggcggatc agtgatagcc atgaggacac tgggattctg
     3181 gacttcagct cactgctgaa aaagagagac agtttccgga ccccgaggga ctcgaagctg
     3241 gaggcaccag cagaggagga cgtgtgggag atcctacggc aggcaccccc atctgagtac
     3301 gagcgcatcg ccttccagta cggcgtcact gacctgcgcg gcatgctaaa gaggctcaag
     3361 ggcatgaggc gcgatgagaa gaagagcaca gcctttcaga agaagctgga gccggcctac
     3421 caggtgagca aaggccacaa gatccggctg accgtggaac tggctgacca tgacgctgag
     3481 gtcaaatggc tcaagaatgg ccaggagatc cagatgagcg gcagcaagta catctttgag
     3541 tccatcggtg ccaagcgtac cctgaccatc agccagtgct cattggcgga cgacgcagcc
     3601 taccagtgcg tggtgggtgg cgagaagtgt agcacggagc tctttgtgaa agagccccct
     3661 gtgctcatca cgcgcccctt ggaggaccag ctggtgatgg tggggcagcg ggtggagttt
     3721 gagtgtgaag tatcggagga gggggcgcaa gtcaaatggc tgaaggacgg ggtggagctg
     3781 acccgggagg agaccttcaa ataccggttc aagaaggacg ggcagagaca ccacctgatc
     3841 atcaacgagg ccatgctgga ggacgcgggg cactatgcac tgtgcactag cgggggccag
     3901 gcgctggctg agctcattgt gcaggaaaag aagctggagg tgtaccagag catcgcagac
     3961 ctgatggtgg gcgcaaagga ccaggcggtg ttcaaatgtg aggtctcaga tgagaatgtt
     4021 cggggtgtgt ggctgaagaa tgggaaggag ctggtgcccg acagccgcat aaaggtgtcc
     4081 cacatcgggc gggtccacaa actgaccatt gacgacgtca cacctgccga cgaggctgac
     4141 tacagctttg tgcccgaggg cttcgcctgc aacctgtcag ccaagctcca cttcatggag
     4201 gtcaagattg acttcgtacc caggcaggaa cctcccaaga tccacctgga ctgcccaggc
     4261 cgcataccag acaccattgt ggttgtagct ggaaataagc tacgtctgga cgtccctatc
     4321 tctggggacc ctgctcccac tgtgatctgg cagaaggcta tcacgcaggg gaataaggcc
     4381 ccagccaggc cagccccaga tgccccagag gacacaggtg acagcgatga gtgggtgttt
     4441 gacaagaagc tgctgtgtga gaccgagggc cgggtccgcg tggagaccac caaggaccgc
     4501 agcatcttca cggtcgaggg ggcagagaag gaagatgagg gcgtctacac ggtcacagtg
     4561 aagaaccctg tgggcgagga ccaggtcaac ctcacagtca aggtcatcga cgtgccagac
     4621 gcacctgcgg cccccaagat cagcaacgtg ggagaggact cctgcacagt acagtgggag
     4681 ccgcctgcct acgatggcgg gcagcccatc ctgggctaca tcctggagcg caagaagaag
     4741 aagagctacc ggtggatgcg gctgaacttc gacctgattc aggagctgag tcatgaagcg
     4801 cggcgcatga tcgagggcgt ggtgtacgag atgcgcgtct acgcggtcaa cgccatcggc
     4861 atgtccaggc ccagccctgc ctcccagccc ttcatgccta tcggtccccc cagcgaaccc
     4921 acccacctgg cagtagagga cgtctctgac accacggtct ccctcaagtg gcggccccca
     4981 gagcgcgtgg gagcaggagg cctggatggc tacagcgtgg agtactgccc agagggctgc
     5041 tcagagtggg tggctgccct gcaggggctg acagagcaca catcgatact ggtgaaggac
     5101 ctgcccacgg gggcccggct gcttttccga gtgcgggcac acaatatggc agggcctgga
     5161 gcccctgtta ccaccacgga gccggtgaca gtgcaggaga tcctgcaacg gccacggctt
     5221 cagctgccca ggcacctgcg ccagaccatt cagaagaagg tcggggagcc tgtgaacctt
     5281 ctcatccctt tccagggcaa gccccggcct caggtgacct ggaccaaaga ggggcagccc
     5341 ctggcaggcg aggaggtgag catccgcaac agccccacag acaccatcct gttcatccgg
     5401 gccgctcgcc gcgtgcattc aggcacttac caggtgacgg tgcgcattga gaacatggag
     5461 gacaaggcca cgctggtgct gcaggttgtt gacaagccaa gtcctcccca ggatctccgg
     5521 gtgactgacg cctggggtct taatgtggct ctggagtgga agccacccca ggatgtcggc
     5581 aacacggagc tctgggggta cacagtgcag aaagccgaca agaagaccat ggagtggttc
     5641 accgtcttgg agcattaccg ccgcacccac tgcgtggtgc cagagctcat cattggcaat
     5701 ggctactact tccgcgtctt cagccagaat atggttggct ttagtgacag agcggccacc
     5761 accaaggagc ccgtctttat ccccagacca ggcatcacct atgagccacc caactataag
     5821 gccctggact tctccgaggc cccaagcttc acccagcccc tggtgaaccg ctcggtcatc
     5881 gcgggctaca ctgctatgct ctgctgtgct gtccggggta gccccaagcc caagatttcc
     5941 tggttcaaga atggcctgga cctgggagaa gacgcccgct tccgcatgtt cagcaagcag
     6001 ggagtgttga ctctggagat tagaaagccc tgcccctttg acgggggcat ctatgtctgc
     6061 agggccacca acttacaggg cgaggcacgg tgtgagtgcc gcctggaggt gcgagtgcct
     6121 cagggcggag gtggttcagg tggcggaggt tctggatcca tggtgtctaa gggcgaagag
     6181 ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgaa caaccaccac
     6241 ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc
     6301 aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg
     6361 tacggcagca gaaccttcat caaccacacc cagggcatcc ccgacttctt taagcagtcc
     6421 ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc
     6481 gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg
     6541 gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccaac
     6601 accgagatgc tgtaccccgc tgacggcggc ctggaaggca gaagcgacat ggccctgaag
     6661 ctcgtgggcg ggggccacct gatctgcaac ttcaagacca catacagatc caagaaaccc
     6721 gctaagaacc tcaagatgcc cggcgtctac tatgtggacc acagactgga aagaatcaag
     6781 gaggccgaca aagagaccta cgtcgagcag cacgaggtgg ctgtggccag atactgcgac
     6841 ctccctagca aactggggca caaacttaat tgagcggccg cgaaggatct gcgatcgctc
     6901 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     6961 ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     7021 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     7081 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg
     7141 ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt
     7201 gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt
     7261 aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag
     7321 actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc
     7381 gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagatgaccg
     7441 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc
     7501 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca
     7561 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     7621 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     7681 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     7741 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     7801 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     7861 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     7921 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     7981 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagtcgaca
     8041 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     8101 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     8161 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     8221 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     8281 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     8341 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     8401 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     8461 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     8521 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     8581 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta
     8641 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     8701 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct
     8761 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     8821 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     8881 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     8941 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     9001 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     9061 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     9121 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt
     9181 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     9241 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg
     9301 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     9361 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     9421 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     9481 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     9541 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     9601 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     9661 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     9721 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     9781 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     9841 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     9901 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     9961 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    10021 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    10081 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    10141 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
    10201 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    10261 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    10321 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    10381 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    10441 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    10501 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    10561 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    10621 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    10681 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    10741 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    10801 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    10861 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    10921 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    10981 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    11041 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    11101 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    11161 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    11221 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    11281 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    11341 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    11401 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    11461 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
    11521 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
    11581 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
    11641 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
    11701 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
    11761 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
    11821 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
    11881 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca
    11941 agctg
//
