LOCUS       V029410                10573 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029410
VERSION     V029410
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4599
                     /label="MYB(NM_001130173)"
                     /note="MYB(NM_001130173)"
                     /gene="MYB"
     CDS             4606..4671
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4715..4926
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4939..5207
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5236..5245
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5266..5928
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5998..6051
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6052..6651
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6652..7240
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7314..7547
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7619..7740
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7780..7915
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7948..7964)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7972..7988
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7996..8026)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8041..8062
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8350..8938)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9109..9969)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9970..10074)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10548..10564
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cccgaagacc ccggcacagc
     2341 atatatagca gtgacgagga tgatgaggac tttgagatgt gtgaccatga ctatgatggg
     2401 ctgcttccca agtctggaaa gcgtcacttg gggaaaacaa ggtggacccg ggaagaggat
     2461 gaaaaactga agaagctggt ggaacagaat ggaacagatg actggaaagt tattgccaat
     2521 tatctcccga atcgaacaga tgtgcagtgc cagcaccgat ggcagaaagt actaaaccct
     2581 gagctcatca agggtccttg gaccaaagaa gaagatcaga gagtgataga gcttgtacag
     2641 aaatacggtc cgaaacgttg gtctgttatt gccaagcact taaaggggag aattggaaaa
     2701 caatgtaggg agaggtggca taaccacttg aatccagaag ttaagaaaac ctcctggaca
     2761 gaagaggaag acagaattat ttaccaggca cacaagagac tggggaacag atgggcagaa
     2821 atcgcaaagc tactgcctgg acgaactgat aatgctatca agaaccactg gaattctaca
     2881 atgcgtcgga aggtcgaaca ggaaggttat ctgcaggagt cttcaaaagc cagccagcca
     2941 gcagtggcca caagcttcca gaagaacagt catttgatgg gttttgctca ggctccgcct
     3001 acagctcaac tccctgccac tggccagccc actgttaaca acgactattc ctattaccac
     3061 atttctgaag cacaaaatgt ctccagtcat gttccatacc ctgtagcgtt acatgtaaat
     3121 atagtcaatg tccctcagcc agctgccgca gccattcaga gacactataa tgatgaagac
     3181 cctgagaagg aaaagcgaat aaaggaatta gaattgctcc taatgtcaac cgagaatgag
     3241 ctaaaaggac agcaggtgct accaacacag aaccacacat gcagctaccc cgggtggcac
     3301 agcaccacca ttgccgacca caccagacct catggagaca gtgcacctgt ttcctgtttg
     3361 ggagaacacc actccactcc atctctgcca gcggatcctg gctccctacc tgaagaaagc
     3421 gcctcgccag caaggtgcat gatcgtccac cagggcacca ttctggataa tgttaagaac
     3481 ctcttagaat ttgcagaaac actccaattt atagattctg attcttcatc atggtgtgat
     3541 ctcagcagtt ttgaattctt tgaagaagca gatttttcac ctagccaaca tcacacaggc
     3601 aaagccctac agcttcagca aagagagggc aatgggacta aacctgcagg agaacctagc
     3661 ccaagggtga acaaacgtat gttgagtgag agttcacttg acccacccaa ggtcttacct
     3721 cctgcaaggc acagcacaat tccactggtc atccttcgaa aaaaacgggg ccaggccagc
     3781 cccttagcca ctggagactg tagctccttc atatttgctg acgtcagcag ttcaactccc
     3841 aagcgttccc ctgtcaaaag cctacccttc tctccctcgc agttcttaaa cacttccagt
     3901 aaccatgaaa actcagactt ggaaatgcct tctttaactt ccacccccct cattggtcac
     3961 aaattgactg ttacaacacc atttcataga gaccagactg tgaaaactca aaaggaaaat
     4021 actgttttta gaaccccagc tatcaaaagg tcaatcttag aaagctctcc aagaactcct
     4081 acaccattca aacatgcact tgcagctcaa gaaattaaat acggtcccct gaagatgcta
     4141 cctcagacac cctctcatct agtagaagat ctgcaggatg tgatcaaaca ggaatctgat
     4201 gaatctggaa ttgttgctga gtttcaagaa aatggaccac ccttactgaa gaaaatcaaa
     4261 caagaggtgg aatctccaac tgataaatca ggaaacttct tctgctcaca ccactgggaa
     4321 ggggacagtc tgaataccca actgttcacg cagacctcgc ctgtggcaga tgcaccgaat
     4381 attcttacaa gctccgtttt aatggcacca gcatcagaag atgaagacaa tgttctcaaa
     4441 gcatttacag tacctaaaaa caggtccctg gcgagcccct tgcagccttg tagcagtacc
     4501 tgggaacctg catcctgtgg aaagatggag gagcagatga catcttccag tcaagctcgt
     4561 aaatacgtga atgcattctc agcccggacg ctggtcatgg gatccgacta caaagaccat
     4621 gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa gtagcggccg
     4681 cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     4741 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg
     4801 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     4861 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     4921 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga
     4981 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga
     5041 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg
     5101 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct
     5161 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg
     5221 gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc
     5281 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag
     5341 ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc
     5401 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac
     5461 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc
     5521 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag
     5581 gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg
     5641 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc
     5701 gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac
     5761 agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag
     5821 aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg
     5881 ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct
     5941 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag
     6001 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag
     6061 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     6121 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     6181 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     6241 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     6301 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     6361 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     6421 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     6481 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     6541 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     6601 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct
     6661 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     6721 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     6781 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     6841 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     6901 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     6961 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     7021 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     7081 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     7141 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     7201 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat
     7261 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     7321 gctaattcac tcccaacgaa gataagatct gctttttgct tgtactgggt ctctctggtt
     7381 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7441 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7501 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     7561 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     7621 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7681 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     7741 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     7801 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     7861 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac
     7921 ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
     7981 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8041 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8101 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8161 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8221 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8281 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8341 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8401 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8461 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8521 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8581 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8641 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8701 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     8761 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     8821 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8881 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8941 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9001 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     9061 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9121 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9181 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9241 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9301 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9361 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9421 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9481 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9541 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9601 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9661 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     9721 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     9781 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     9841 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9901 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9961 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10021 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    10081 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
    10141 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
    10201 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
    10261 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
    10321 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    10381 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
    10441 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    10501 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    10561 cagtgccaag ctg
//
