LOCUS       V028086                11338 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028086
VERSION     V028086
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5364
                     /label="MTCL2(NM_199181)"
                     /note="MTCL2(NM_199181)"
                     /gene="MTCL2"
     CDS             5371..5436
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5480..5691
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5704..5972
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6001..6010
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6031..6693
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6763..6816
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6817..7416
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7417..8005
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8079..8312
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8384..8505
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8545..8680
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8713..8729)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8737..8753
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8761..8791)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8806..8827
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9115..9703)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9874..10734)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10735..10839)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11313..11329
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc tggagatgcg ggacgtctat
     2341 atggaggagg acgtgtatca gctgcaggag ctgcgacagc agctggacca ggccagcaag
     2401 acctgccgca tcctgcagta ccggctgcgc aaagccgagc gccgcagtct ccgtgccgcc
     2461 cagaccggcc aggtggacgg cgagcttatc cgtggtctgg agcaggatgt caaggtctct
     2521 aaggacatct ccatgcggct gcataaggag ctcgaggtgg tggagaagaa acgggcgcgg
     2581 ctggaggagg agaacgaaga gcttcgtcag cggctcatcg agactgagct ggctaagcag
     2641 gtgctgcaga cggagctgga gcgaccgaga gagcattcct tgaagaaaag aggaacccgc
     2701 tccctgggga aggccgataa gaagactttg gtgcaggagg acagtgcaga cctgaagtgc
     2761 cagttgcact ttgcaaagga ggagtcagcc ctcatgtgca agaagctcac taagcttgcc
     2821 aaggagaatg acagcatgaa ggaggagctg ctgaagtacc gctcgctcta tggggacctg
     2881 gacagcgcgc tgtcagccga ggagctggcc gatgcccccc actcgcggga gaccgagctg
     2941 aaggtgcacc tgaagctggt ggaggaggaa gccaacctgc tgagccgccg catcgtggag
     3001 ctggaggtgg agaaccgagg cctgcgggct gagatggacg acatgaagga tcatggaggt
     3061 ggctgtgggg gtcctgaggc acgcctggcc ttctccgcgc tgggtggcgg agagtgcggg
     3121 gagagcttgg cagagctgcg gcgacacctg cagtttgtcg aagaggaggc cgagctgctg
     3181 cggcgctcct ctgccgagct cgaggaccag aacaagctgc tgctgaacga gctggccaag
     3241 ttccgctcgg agcacgagct ggacgtggcg ctgtcggagg acagttgttc tgtgctcagc
     3301 gaaccttcac aggaggagct ggcggccgcc aagctgcaga tcggcgagct cagcggcaag
     3361 gtcaagaagc tgcagtacga gaaccgcgtg ctcctctcca acctccagcg ctgtgacctc
     3421 gcctcctgcc agagtacgcg gcccatgctg gagacggacg ccgaggccgg ggactctgcc
     3481 cagtgtgtgc ctgctcccct gggcgagaca cacgagtccc atgcggtccg actctgcaga
     3541 gccagggagg ccgaggtgct gcctgggctg agagagcagg ccgccctggt cagtaaggcc
     3601 atcgatgtcc tggtggctga tgccaatggc ttcacggctg gcctccggct gtgtctggac
     3661 aacgagtgtg ctgacttccg gctgcatgag gcccccgaca acagcgaggg ccccagggac
     3721 accaagctca tccatgccat cctggtgcgc ctgagcgtgc tgcagcagga gctgaatgcc
     3781 ttcacgcgga aggcagatgc agtcctcggg tgctctgtca aggaacagca ggagtccttc
     3841 tcatcactgc cccccttggg ctcccagggg ctctctaagg agattcttct ggcaaaagac
     3901 cttggctcag actttcagcc acctgacttc agggacctgc cggaatggga gcccaggatc
     3961 cgagaggctt tccgcactgg tgacttggac tctaagcccg accccagccg gagcttcagg
     4021 ccttaccgag ctgaagacaa tgattcctat gcctctgaga tcaaggagct gcagctggtg
     4081 ctggctgagg cccacgacag cctccggggc ttgcaagagc agctctccca ggagcggcag
     4141 ctacgaaagg aggaggccga caatttcaac cagaaaatgg tccagctgaa ggaggaccag
     4201 cagagggcgc tcctgaggcg ggagtttgag ctgcagagtc tgagcctcca gcggaggctg
     4261 gagcagaaat tctggagcca ggagaagaac atgctggtgc aggagtccca gcaattcaag
     4321 cacaacttcc tgctgctctt catgaagctc aggtggttcc tcaagcgctg gcggcagggc
     4381 aaggttttgc ccagcgaagg ggatgacttc ctcgaggtga acagcatgaa ggagctgtac
     4441 ttgctgatgg aggaagagga gataaacgct cagcattctg ataacaaggc ctgcacgggg
     4501 gacagctgga cccagaacac gcccaatgag tacatcaaga cactggccga catgaaggtg
     4561 acgctgaagg agctgtgctg gctgctccgg gatgaacgcc gtggtctgac ggagcttcag
     4621 caacagtttg ccaaggccaa ggctacctgg gagacagagc gggcagagct caagggccat
     4681 acctcccaga tggagctgaa gacagggaag ggggccgggg agcgggcagg gcccgactgg
     4741 aaggcagccc tacagcggga gcgtgaggag cagcagcacc tcctagctga gtcctacagc
     4801 gctgtcatgg agctgactcg gcagctgcag atcagtgagc gcaactggag ccaggaaaag
     4861 ctgcagctgg tggagcggct gcagggtgag aagcagcagg tggagcagca ggtgaaggag
     4921 ctgcagaacc gcctaagcca gctgcagaag gctgccgacc cctgggtcct gaagcactcg
     4981 gagctggaga agcaggacaa cagctggaag gagacacgca gtgagaagat ccacgacaag
     5041 gaggctgttt ccgaagttga gcttggagga aatggtttaa agagaaccaa atctgtttct
     5101 tccatgtctg agtttgaaag tttgctcgac tgttcccctt accttgctgg cggagatgcc
     5161 cggggcaaga agctgcctaa caaccctgcc tttggctttg tgagctccga gccaggggat
     5221 ccagagaaag acaccaagga gaagcctggg ctctcgtcga gggactgcaa ccacctgggt
     5281 gccctggcct gccaggaccc cccagggagc cagaagctgc ccttcctcct catcctggcc
     5341 cctccccagc ccccgccaat actgggatcc gactacaaag accatgacgg tgattataaa
     5401 gatcatgaca tcgactacaa ggatgacgat gacaagtagc ggccgcgaag gatctgcgat
     5461 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     5521 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     5581 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     5641 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt
     5701 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
     5761 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
     5821 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
     5881 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
     5941 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac
     6001 gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc
     6061 ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag
     6121 ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg
     6181 ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag
     6241 aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac
     6301 gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc
     6361 ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag
     6421 atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg
     6481 ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac
     6541 agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg
     6601 ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac
     6661 cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct
     6721 gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag aggaagtctt
     6781 ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg
     6841 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     6901 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     6961 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     7021 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     7081 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     7141 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     7201 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     7261 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     7321 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     7381 cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt
     7441 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     7501 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     7561 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     7621 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     7681 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     7741 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     7801 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     7861 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     7921 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     7981 atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc
     8041 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     8101 acgaagataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     8161 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     8221 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     8281 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     8341 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     8401 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     8461 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     8521 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     8581 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     8641 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagaccaa
     8701 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     8761 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     8821 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     8881 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     8941 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     9001 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     9061 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     9121 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     9181 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     9241 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     9301 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     9361 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     9421 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     9481 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     9541 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     9601 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     9661 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     9721 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     9781 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     9841 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     9901 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     9961 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    10021 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    10081 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    10141 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    10201 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    10261 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    10321 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    10381 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    10441 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    10501 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    10561 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    10621 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    10681 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    10741 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    10801 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    10861 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
    10921 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
    10981 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
    11041 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
    11101 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
    11161 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
    11221 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
    11281 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg
//
