LOCUS V028086 11338 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V028086 VERSION V028086 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2317..5364 /label="MTCL2(NM_199181)" /note="MTCL2(NM_199181)" /gene="MTCL2" CDS 5371..5436 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 5480..5691 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 5704..5972 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 6001..6010 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 6031..6693 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 6763..6816 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 6817..7416 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 7417..8005 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 8079..8312 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 8384..8505 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 8545..8680 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(8713..8729) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 8737..8753 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(8761..8791) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8806..8827 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(9115..9703) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9874..10734) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(10735..10839) /gene="bla" /label="AmpR promoter" primer_bind 11313..11329 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc tggagatgcg ggacgtctat 2341 atggaggagg acgtgtatca gctgcaggag ctgcgacagc agctggacca ggccagcaag 2401 acctgccgca tcctgcagta ccggctgcgc aaagccgagc gccgcagtct ccgtgccgcc 2461 cagaccggcc aggtggacgg cgagcttatc cgtggtctgg agcaggatgt caaggtctct 2521 aaggacatct ccatgcggct gcataaggag ctcgaggtgg tggagaagaa acgggcgcgg 2581 ctggaggagg agaacgaaga gcttcgtcag cggctcatcg agactgagct ggctaagcag 2641 gtgctgcaga cggagctgga gcgaccgaga gagcattcct tgaagaaaag aggaacccgc 2701 tccctgggga aggccgataa gaagactttg gtgcaggagg acagtgcaga cctgaagtgc 2761 cagttgcact ttgcaaagga ggagtcagcc ctcatgtgca agaagctcac taagcttgcc 2821 aaggagaatg acagcatgaa ggaggagctg ctgaagtacc gctcgctcta tggggacctg 2881 gacagcgcgc tgtcagccga ggagctggcc gatgcccccc actcgcggga gaccgagctg 2941 aaggtgcacc tgaagctggt ggaggaggaa gccaacctgc tgagccgccg catcgtggag 3001 ctggaggtgg agaaccgagg cctgcgggct gagatggacg acatgaagga tcatggaggt 3061 ggctgtgggg gtcctgaggc acgcctggcc ttctccgcgc tgggtggcgg agagtgcggg 3121 gagagcttgg cagagctgcg gcgacacctg cagtttgtcg aagaggaggc cgagctgctg 3181 cggcgctcct ctgccgagct cgaggaccag aacaagctgc tgctgaacga gctggccaag 3241 ttccgctcgg agcacgagct ggacgtggcg ctgtcggagg acagttgttc tgtgctcagc 3301 gaaccttcac aggaggagct ggcggccgcc aagctgcaga tcggcgagct cagcggcaag 3361 gtcaagaagc tgcagtacga gaaccgcgtg ctcctctcca acctccagcg ctgtgacctc 3421 gcctcctgcc agagtacgcg gcccatgctg gagacggacg ccgaggccgg ggactctgcc 3481 cagtgtgtgc ctgctcccct gggcgagaca cacgagtccc atgcggtccg actctgcaga 3541 gccagggagg ccgaggtgct gcctgggctg agagagcagg ccgccctggt cagtaaggcc 3601 atcgatgtcc tggtggctga tgccaatggc ttcacggctg gcctccggct gtgtctggac 3661 aacgagtgtg ctgacttccg gctgcatgag gcccccgaca acagcgaggg ccccagggac 3721 accaagctca tccatgccat cctggtgcgc ctgagcgtgc tgcagcagga gctgaatgcc 3781 ttcacgcgga aggcagatgc agtcctcggg tgctctgtca aggaacagca ggagtccttc 3841 tcatcactgc cccccttggg ctcccagggg ctctctaagg agattcttct ggcaaaagac 3901 cttggctcag actttcagcc acctgacttc agggacctgc cggaatggga gcccaggatc 3961 cgagaggctt tccgcactgg tgacttggac tctaagcccg accccagccg gagcttcagg 4021 ccttaccgag ctgaagacaa tgattcctat gcctctgaga tcaaggagct gcagctggtg 4081 ctggctgagg cccacgacag cctccggggc ttgcaagagc agctctccca ggagcggcag 4141 ctacgaaagg aggaggccga caatttcaac cagaaaatgg tccagctgaa ggaggaccag 4201 cagagggcgc tcctgaggcg ggagtttgag ctgcagagtc tgagcctcca gcggaggctg 4261 gagcagaaat tctggagcca ggagaagaac atgctggtgc aggagtccca gcaattcaag 4321 cacaacttcc tgctgctctt catgaagctc aggtggttcc tcaagcgctg gcggcagggc 4381 aaggttttgc ccagcgaagg ggatgacttc ctcgaggtga acagcatgaa ggagctgtac 4441 ttgctgatgg aggaagagga gataaacgct cagcattctg ataacaaggc ctgcacgggg 4501 gacagctgga cccagaacac gcccaatgag tacatcaaga cactggccga catgaaggtg 4561 acgctgaagg agctgtgctg gctgctccgg gatgaacgcc gtggtctgac ggagcttcag 4621 caacagtttg ccaaggccaa ggctacctgg gagacagagc gggcagagct caagggccat 4681 acctcccaga tggagctgaa gacagggaag ggggccgggg agcgggcagg gcccgactgg 4741 aaggcagccc tacagcggga gcgtgaggag cagcagcacc tcctagctga gtcctacagc 4801 gctgtcatgg agctgactcg gcagctgcag atcagtgagc gcaactggag ccaggaaaag 4861 ctgcagctgg tggagcggct gcagggtgag aagcagcagg tggagcagca ggtgaaggag 4921 ctgcagaacc gcctaagcca gctgcagaag gctgccgacc cctgggtcct gaagcactcg 4981 gagctggaga agcaggacaa cagctggaag gagacacgca gtgagaagat ccacgacaag 5041 gaggctgttt ccgaagttga gcttggagga aatggtttaa agagaaccaa atctgtttct 5101 tccatgtctg agtttgaaag tttgctcgac tgttcccctt accttgctgg cggagatgcc 5161 cggggcaaga agctgcctaa caaccctgcc tttggctttg tgagctccga gccaggggat 5221 ccagagaaag acaccaagga gaagcctggg ctctcgtcga gggactgcaa ccacctgggt 5281 gccctggcct gccaggaccc cccagggagc cagaagctgc ccttcctcct catcctggcc 5341 cctccccagc ccccgccaat actgggatcc gactacaaag accatgacgg tgattataaa 5401 gatcatgaca tcgactacaa ggatgacgat gacaagtagc ggccgcgaag gatctgcgat 5461 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg 5521 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt 5581 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca 5641 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt 5701 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc 5761 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc 5821 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta 5881 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt 5941 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac 6001 gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc 6061 ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag 6121 ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg 6181 ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag 6241 aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac 6301 gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc 6361 ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag 6421 atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg 6481 ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac 6541 agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg 6601 ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac 6661 cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct 6721 gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag aggaagtctt 6781 ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg 6841 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc 6901 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag 6961 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac 7021 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc 7081 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag 7141 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc 7201 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg 7261 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc 7321 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg 7381 cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt 7441 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 7501 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 7561 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc 7621 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt 7681 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc 7741 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg 7801 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg 7861 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc 7921 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg 7981 atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc 8041 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca 8101 acgaagataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc 8161 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 8221 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 8281 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca 8341 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc 8401 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 8461 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta 8521 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct 8581 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 8641 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagaccaa 8701 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 8761 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 8821 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 8881 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 8941 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 9001 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 9061 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 9121 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 9181 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 9241 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 9301 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 9361 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 9421 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 9481 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 9541 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 9601 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 9661 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 9721 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 9781 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 9841 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 9901 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 9961 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 10021 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 10081 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 10141 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 10201 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 10261 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 10321 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 10381 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 10441 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 10501 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 10561 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 10621 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 10681 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 10741 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 10801 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 10861 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 10921 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 10981 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 11041 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 11101 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 11161 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 11221 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 11281 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg //