LOCUS V030273 8761 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V030273 VERSION V030273 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..3591 /label="MRPS5(NM_031902)" /note="MRPS5(NM_031902)" /gene="MRPS5" CDS 3598..3687 /codon_start=1 /product="3 tandem Myc epitope tags" /transl_table=1 /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" /label="3xMyc" promoter 3731..3942 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 3955..4223 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 4250..4849 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 4856..5444 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5518..5751 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 5823..5944 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 5963..6098 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6136..6152) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6160..6176 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6184..6214) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6229..6250 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6538..7126) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7297..8157) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8158..8262) /gene="bla" /label="AmpR promoter" primer_bind 8736..8752 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catggcgacc gcggtgcgcg ctgtgggctg cctccccgtg 2341 ctgtgtagcg ggacggcagg tcatttattg gggaggcagt gttccctaaa caccttacca 2401 gcagcttcca ttttggcatg gaagagtgtt ctcggcaatg gccatttgtc atcactggga 2461 accagagaca cccatcccta cgccagcttg agccgtgcac tgcagacaca atgctgtatt 2521 tcttctccca gtcacctgat gagccagcag tatagaccat atagtttctt cactaaattg 2581 actgcagatg agctgtggaa aggcgcttta gcagagactg gtgctggagc aaaaaaagga 2641 agaggcaaaa gaactaaaaa gaagaaaaga aaggatctga acaggggtca gatcattggt 2701 gaagggcgtt atggttttct atggcccgga ctgaatgtcc ctcttatgaa aaatggagca 2761 gtgcagacca ttgcccaaag aagcaaggaa gagcaggaga aggtggaggc agacatgatc 2821 cagcagagag aagagtggga ccgaaagaag aagatgaagg ttaaacggga gcgaggatgg 2881 agtggaaact catggggagg catcagtctt ggcccccctg accctggtcc ctgtggagaa 2941 acatatgagg attttgatac caggatactt gaggtaagaa acgttttcac tatgactgcg 3001 aaagagggaa gaaagaaatc gatccgtgtc ttggtggctg tggggaacgg aaaaggagct 3061 gcaggttttt ctattgggaa agctactgat cggatggatg ctttcaggaa agcaaagaac 3121 agagcagttc accatttgca ttatatagaa cgatatgaag accatacaat attccatgat 3181 atttcattaa gatttaaaag gacgcatatc aagatgaaga aacaacccaa aggttacggc 3241 ctccgctgcc acagggccat catcaccatc tgccggctca ttggcatcaa agacatgtat 3301 gccaaggtct ctgggtccat taatatgctc agcctcaccc agggcctctt ccgtgggctc 3361 tccagacagg aaacccatca acagctggct gataagaagg gcctccatgt tgtggaaatc 3421 cgggaggaat gtggccctct gcccattgtg gttgcgtccc cccgggggcc cttgaggaag 3481 gatccagagc cagaagatga ggttccagac gtcaaactgg actgggaaga tgtgaagact 3541 gcacagggaa tgaagcgctc tgtgtggtct aatttgaaga gagccgccac gggatccgaa 3601 caaaaactaa tatcagagga agatttggaa cagaaactaa tcagtgaaga agatttagag 3661 caaaagttaa tttccgagga ggacttatag cggccgcgaa ggatctgcga tcgctccggt 3721 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc 3781 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg 3841 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc 3901 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct 3961 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt 4021 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt 4081 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc 4141 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt 4201 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga tgaccgagta 4261 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc 4321 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga 4381 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt 4441 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc 4501 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc 4561 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt 4621 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt 4681 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc 4741 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt 4801 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag tcgacaatca 4861 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 4921 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 4981 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 5041 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 5101 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc 5161 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 5221 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg 5281 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 5341 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct 5401 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac 5461 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg 5521 aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact gggtctctct 5581 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc 5641 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 5701 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt 5761 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga 5821 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 5881 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 5941 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc 6001 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 6061 gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca gagacggccc 6121 aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 6181 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 6241 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 6301 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 6361 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 6421 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 6481 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 6541 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 6601 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 6661 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 6721 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 6781 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 6841 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 6901 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 6961 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 7021 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 7081 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 7141 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 7201 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 7261 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 7321 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 7381 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 7441 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 7501 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 7561 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 7621 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 7681 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 7741 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 7801 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 7861 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 7921 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 7981 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 8041 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 8101 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 8161 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 8221 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 8281 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 8341 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca 8401 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca 8461 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca 8521 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa 8581 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt 8641 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag 8701 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct 8761 g //