LOCUS       V036515                10425 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036515
VERSION     V036515
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..4507
                     /label="MID2(NM_012216)"
                     /note="MID2(NM_012216)"
                     /gene="MID2"
     CDS             4514..4579
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4623..4834
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4847..5115
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5150..5857
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             5867..5920
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5921..6520
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6521..7108
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7182..7415
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7487..7608
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7627..7762
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7800..7816)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7824..7840
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7848..7878)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7893..7914
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8202..8790)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8961..9821)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9822..9926)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10400..10416
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgggtga aagcccagcc tccgtggttc ttaatgcctc
     2341 aggaggacta ttttcactaa agatggaaac actggagtct gaattgacct gtccaatctg
     2401 cctagagttg tttgaagacc cccttctgct cccttgtgct cacagcctct gcttcagctg
     2461 tgcccatcgc attttggtat caagctgcag ctctggtgaa tccattgaac ccattactgc
     2521 tttccagtgt cctacctgca ggtatgttat ctcgctgaac caccggggcc tggatggcct
     2581 caagaggaat gtgactctgc agaacattat tgatcgcttc cagaaggctt cagtcagtgg
     2641 gcccaattcc cctagtgaga gccgccggga aaggacttac aggcccacca ctgccatgtc
     2701 tagcgagcga attgcttgcc aattctgtga gcaggacccg ccaagggatg cagtaaaaac
     2761 atgcatcacc tgtgaggtct cctactgtga ccgttgcctg cgggccacgc accccaacaa
     2821 gaaacctttc accagccacc gcctggtgga accagtgcca gacacacatc ttcgagggat
     2881 cacctgcctg gaccatgaga atgagaaagt gaacatgtac tgtgtatctg atgaccaatt
     2941 gatctgtgcc ttatgcaaac tggtgggtcg tcaccgagac catcaggtcg catccctgaa
     3001 tgatcgattt gagaaactca agcaaactct ggagatgaac ctcaccaacc tggttaagcg
     3061 caacagcgaa ctagaaaatc aaatggccaa actaatacag atctgccagc aggttgaggt
     3121 gaatactgct atgcatgagg caaaacttat ggaagaatgt gacgagttgg tagagatcat
     3181 ccagcagagg aagcaaatga tcgctgtcaa aatcaaagag acaaaggtta tgaaactgag
     3241 aaagttggca cagcaggttg ctaattgccg ccagtgtctt gaacggtcaa cagtcctcat
     3301 caaccaagct gagcatatcc tgaaagaaaa tgaccaggca cggtttctac agtctgcaaa
     3361 aaatattgct gagagggtcg ctatggcaac tgcatcttct caagttctga ttccagacat
     3421 caattttaat gatgcctttg aaaactttgc tttagatttt tccagagaaa agaaactgct
     3481 agaggggtta gattatttaa cagccccaaa cccaccatct atccgagaag aactctgtac
     3541 tgcctcccat gacaccatta cagtccactg gatctcggat gatgagttca gcatcagctc
     3601 ctatgagctt cagtacacca tattcactgg ccaggctaac ttcatcagta agtcatggtg
     3661 tagttggggc ctgtggccag agataaggaa atgtaaggaa gcagtaagct gctcaagatt
     3721 ggccggggcg ccacgaggcc tgtataattc agtagacagc tggatgattg ttcccaacat
     3781 taaacagaac cattacacag tgcatggact ccagagcggg actcgctaca tcttcatcgt
     3841 taaagccata aaccaagccg gcagccggaa cagtgaacct acccgactaa aaacaaacag
     3901 ccaacccttt aaattggatc ccaaaatgac tcacaagaag ttgaagatct ccaatgatgg
     3961 attgcagatg gagaaggatg aaagctctct aaagaagagc cacaccccag agaggtttag
     4021 tggcacaggg tgctatgggg cagcaggaaa tatattcatt gacagtggct gccactattg
     4081 ggaggtggtc atgggttcct caacatggta tgcaattggc attgcctaca aatcagctcc
     4141 aaagaatgaa tggattggca agaatgcctc ctcatgggtc ttctctcgct gcaatagtaa
     4201 cttcgtggtg agacacaaca acaaggaaat gctggtggat gtgcccccac acctgaagcg
     4261 tctgggtgtc ctcctggatt atgacaacaa tatgctgtct ttctatgacc cagctaactc
     4321 tctccatctt catacttttg atgtgacctt cattcttcca gtttgtccaa catttacaat
     4381 ctggaacaaa tccctaatga tcctgtctgg cttgcctgcc ccagatttta ttgattaccc
     4441 tgagcggcag gaatgcaact gcaggcctca agaatcccct tatgtttctg ggatgaaaac
     4501 ctgtcatgga tccgactaca aagaccatga cggtgattat aaagatcatg acatcgacta
     4561 caaggatgac gatgacaagt agcggccgcg aaggatctgc gatcgctccg gtgcccgtca
     4621 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     4681 aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     4741 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     4801 tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc
     4861 tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct
     4921 gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct
     4981 caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct
     5041 ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct
     5101 gcgccgttac agatccaagc tgtgaccggc gcctacgcta gacgccacca tggtgagcaa
     5161 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
     5221 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     5281 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
     5341 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
     5401 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     5461 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     5521 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     5581 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
     5641 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
     5701 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
     5761 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
     5821 ccgccactcc accggcggca tggacgagct gtacaaggga tctcgcgagg gcagaggaag
     5881 tcttctaaca tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac
     5941 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     6001 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     6061 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     6121 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     6181 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     6241 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     6301 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     6361 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     6421 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     6481 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa
     6541 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     6601 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     6661 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     6721 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     6781 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     6841 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     6901 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     6961 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     7021 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc cgccttcgcc ctcagacgag
     7081 tcggatctcc ctttggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc
     7141 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     7201 ccaacgaaaa taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     7261 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     7321 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     7381 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     7441 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     7501 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     7561 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     7621 ctagctatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     7681 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     7741 tgaggaggct tttttggagg cctagacttt tgcagagacg gcccaaattc gtaatcatgg
     7801 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     7861 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     7921 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     7981 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     8041 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     8101 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     8161 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8221 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8281 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8341 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8401 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8461 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8521 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8581 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8641 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8701 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8761 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8821 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8881 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8941 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     9001 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     9061 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9121 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9181 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9241 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9301 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9361 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9421 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9481 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9541 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9601 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9661 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9721 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9781 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9841 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9901 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     9961 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
    10021 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
    10081 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
    10141 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
    10201 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
    10261 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
    10321 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
    10381 ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg
//
