LOCUS       V032562                11478 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032562
VERSION     V032562
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..5497
                     /label="MICAL1(NM_022765)"
                     /note="MICAL1(NM_022765)"
                     /gene="MICAL1"
     CDS             5504..5593
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        5637..5848
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5861..6129
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6158..6167
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6188..6850
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6920..6973
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6974..7573
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7574..8161
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8235..8468
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8540..8661
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8680..8815
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8853..8869)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8877..8893
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8901..8931)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8946..8967
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9255..9843)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10014..10874)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10875..10979)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11453..11469
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgg cttcacctac ctccaccaac ccagcgcatg cccactttga
     2341 gagcttcctg caggcccagc tgtgccagga cgtgctgagc agcttccagg agctgtgtgg
     2401 ggccctgggg ctggaacccg gtggggggct gccccagtac cacaagatca aggaccagct
     2461 caactactgg agcgccaagt cactgtggac caagctggac aagcgagcag gccagcctgt
     2521 ctaccagcag ggccgggcct gcaccagcac caagtgcctg gtggtgggtg ctggaccttg
     2581 cgggctgcgg gtcgctgtgg agctggcgct gctgggggcc cgagtggtgc tggtggaaaa
     2641 gcgcaccaag ttctctcgcc acaacgtgct ccacctctgg cccttcacca tccacgacct
     2701 gcgggcactc ggtgctaaga agttctacgg gcgcttctgc accggcaccc tggaccacat
     2761 cagcatcagg cagctccagc tgcttctgct gaaggtagca ttgctgctgg gggtggaaat
     2821 tcactggggt gtcactttca ctggcctcca gccccctcct aggaagggga gtggctggcg
     2881 tgcccagctc caacccaacc cccctgccca gctggccaac tatgaatttg acgtccttat
     2941 ctcggctgca ggaggtaaat tcgtccctga aggcttcaaa gttcgagaaa tgcgaggcaa
     3001 actggccatt ggcatcacag ccaactttgt gaatggacgc accgtggagg agacacaggt
     3061 gccggagatc agtggtgtag ccaggatcta caaccagagc ttcttccaga gccttctcaa
     3121 agccacaggc attgatctgg agaacattgt gtactacaag gacgacaccc actactttgt
     3181 gatgacagcc aagaagcagt gcctgctgcg gctgggggtg ctgcgccagg actggccaga
     3241 caccaatcgg ctgctgggca gtgccaatgt ggtgcccgag gctctgcagc gctttacccg
     3301 ggcagctgct gactttgcca cccatggcaa gctcgggaaa ctagagtttg cccaggatgc
     3361 ccatgggcag cctgatgtct ctgcctttga cttcacgagc atgatgcggg cagagagttc
     3421 tgctcgtgtg caagagaagc atggcgcccg cctgctgctg ggactggtgg gggactgcct
     3481 ggtggagccc ttctggcccc tgggcactgg agtggcacgg ggcttcctgg cagcctttga
     3541 tgcagcctgg atggtgaagc ggtgggcaga gggcgctgag tccctagagg tgttggctga
     3601 gcgtgagagc ctgtaccagc ttctgtcaca gacatcccca gaaaacatgc atcgcaatgt
     3661 ggcccagtat gggctggacc cagccacccg ctaccccaac ctgaacctcc gggcagtgac
     3721 ccccaatcag gtacgagacc tgtatgatgt gctagccaag gagcctgtgc agaggaacaa
     3781 cgacaagaca gatacaggga tgccagccac cgggtcggca ggcacccagg aggagctgct
     3841 acgctggtgc caggagcaga cagctgggta cccgggagtc cacgtctccg atttgtcttc
     3901 ctcctgggct gatgggctag ctctgtgtgc cctggtgtac cggctgcagc ctggcctgct
     3961 ggaaccctca gagctgcagg ggctgggagc tctggaagca actgcttggg cactaaaggt
     4021 ggcagagaat gagctgggca tcacaccggt ggtgtctgca caggccgtgg tagcagggag
     4081 tgacccactg ggcctcattg cctacctcag ccacttccac agtgccttca agagcatggc
     4141 ccacagccca ggccctgtca gccaggcctc cccagggacc tccagtgctg tattattcct
     4201 tagtaaactt cagaggaccc tgcagcgatc ccgggccaag gaaaatgcag aggatgctgg
     4261 tggcaagaag ctgcgcttgg agatggaggc cgagacccca agtactgagg tgccacctga
     4321 cccagagcct ggtgtacccc tgacaccccc atcccaacac caggaggccg gtgctgggga
     4381 cctgtgtgca ctttgtgggg aacacctcta tgtcctggaa cgcctctgtg tcaacggcca
     4441 tttcttccac cggagctgct tccgctgcca tacctgtgag gccacactgt ggccaggtgg
     4501 ctacgagcag cacccaggag atggacattt ctactgcctc cagcacctgc cccagacaga
     4561 ccacaaagcg gaaggcagcg atagaggccc tgagagtccg gagctcccca caccaagtga
     4621 gaatagcatg ccaccaggcc tctcaactcc cacagcctcg caggaggggg ccggtcctgt
     4681 tccagatccc agccagccca cccgtcggca gatccgcctc tccagcccgg agcgccagcg
     4741 gttgtcctcc cttaacctta cccctgaccc ggaaatggag cctccaccca agcctccccg
     4801 cagctgctcc gccttggccc gccacgccct ggagagcagc tttgtgggct ggggcctgcc
     4861 agtccagagc cctcaagctc ttgtggccat ggagaaggag gaaaaagaga gtcccttctc
     4921 cagtgaagag gaagaagaag atgtgccttt ggactcagat gtggaacagg ccctgcagac
     4981 ctttgccaag acctcaggca ccatgaataa ctacccaaca tggcgtcgga ctctgctgcg
     5041 ccgtgcgaag gaggaggaga tgaagaggtt ctgcaaggcc cagaccatcc aacggcgact
     5101 aaatgagatt gaggctgcct tgagggagct agaggccgag ggcgtgaagc tggagctggc
     5161 cttgaggcgc cagagcagtt ccccagaaca gcaaaagaaa ctatgggtag gacagctgct
     5221 acagctcgtt gacaagaaaa acagcctggt ggctgaggag gccgagctca tgatcacggt
     5281 gcaggaattg aatctggagg agaaacagtg gcagctggac caggagctac gaggctacat
     5341 gaaccgggaa gaaaacctaa agacagctgc tgatcggcag gctgaggacc aggtcctgag
     5401 gaagctggtg gatttggtca accagagaga tgccctcatc cgcttccagg aggagcgcag
     5461 gctcagcgag ctggccttgg ggacaggggc ccagggcgga tccgaacaaa aactaatatc
     5521 agaggaagat ttggaacaga aactaatcag tgaagaagat ttagagcaaa agttaatttc
     5581 cgaggaggac ttatagcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc
     5641 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg
     5701 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     5761 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     5821 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc
     5881 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc
     5941 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc
     6001 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac
     6061 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg
     6121 ttacagatcc aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag
     6181 cggcctgccc gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt
     6241 cgagctggtg ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa
     6301 gagcaccaaa ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg
     6361 cttctaccac ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa
     6421 caacggcggc tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt
     6481 gagcttcagc taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac
     6541 cggcttcccc gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt
     6601 ggagcacctg caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag
     6661 cctgcgcgac ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc
     6721 catccacccc agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga
     6781 gctgcacagc aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat
     6841 cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc
     6901 cggctccacc ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga
     6961 gaatcccggc cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     7021 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     7081 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     7141 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     7201 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     7261 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     7321 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     7381 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     7441 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     7501 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     7561 gcccggtgcc tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     7621 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     7681 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     7741 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     7801 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     7861 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     7921 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     7981 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     8041 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     8101 tcttccgcgt ctccgccttc gccctcagac gagtcggatc tccctttggc cgcctccccg
     8161 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     8221 agaaaagggg ggactggaag ggctaattca ctcccaacga aaataagatc tgctttttgc
     8281 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     8341 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     8401 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     8461 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     8521 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     8581 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     8641 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     8701 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     8761 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac
     8821 ttttgcagag acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     8881 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     8941 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     9001 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     9061 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     9121 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     9181 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     9241 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     9301 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     9361 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     9421 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     9481 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     9541 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     9601 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9661 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     9721 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     9781 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9841 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     9901 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     9961 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    10021 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    10081 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    10141 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    10201 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    10261 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    10321 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    10381 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    10441 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    10501 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    10561 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    10621 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    10681 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10741 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    10801 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10861 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    10921 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    10981 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
    11041 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
    11101 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
    11161 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
    11221 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
    11281 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
    11341 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
    11401 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
    11461 acggccagtg ccaagctg
//
