LOCUS       V023711                 9959 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V023711
VERSION     V023711
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4002
                     /label="METTL16(NM_024086)"
                     /note="METTL16(NM_024086)"
                     /gene="METTL16"
     CDS             4009..4074
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4118..4329
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4342..4610
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4639..4648
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4669..5331
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5401..5454
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5455..6054
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6055..6642
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6716..6949
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7021..7142
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7161..7296
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7334..7350)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7358..7374
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7382..7412)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7427..7448
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7736..8324)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8495..9355)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9356..9460)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9934..9950
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ctctgagtaa atcaatgcat
     2341 gcaagaaata gatacaagga caaacctcct gactttgcat atctggcatc caaatatcca
     2401 gattttaagc agcatgttca gataaatctg aatggaagag tgagccttaa ttttaaagac
     2461 cccgaagcag tcagagctct gacgtgtact ctcctaaggg aagattttgg actttctatt
     2521 gatattccat tggagagact aattcccaca gttcccttga gactcaacta tattcactgg
     2581 gtagaagatc tgatcggtca ccaggattct gacaaaagta ctctccgaag aggaattgac
     2641 ataggcacgg gggcatcttg catctacccc ttacttggag caaccttgaa tggctggtat
     2701 ttcctcgcaa cagaagtgga tgatatgtgt ttcaactatg caaagaaaaa tgtggaacag
     2761 aataacttat ctgatctcat aaaagtggtg aaagtgccac agaagacact cctgatggat
     2821 gctcttaaag aagaatctga gataatctat gacttttgca tgtgcaaccc tccctttttt
     2881 gccaatcaat tggaagccaa gggagtaaac tcacgaaatc ctcgaagacc tccgcctagt
     2941 tctgttaata caggaggcat cacagagatc atggcagaag gaggtgaatt agagtttgtt
     3001 aaaaggatca tccatgacag tctacaactt aaaaaaagat taagatggta tagctgcatg
     3061 ctgggaaaga aatgcagcct ggcgcctctg aaggaggagc ttcgcataca aggggttccc
     3121 aaagtaacgt acactgaatt ctgtcaaggt cggacaatga gatgggcctt agcttggagt
     3181 ttttatgatg atgtcacagt accatcacca ccaagtaagc gaagaaaatt agagaaaccg
     3241 agaaaaccca taacattcgt ggtgctggcg tccgtgatga aggaattatc cctcaaagca
     3301 tcacctctgc gctcggagac ggcggaaggc atagtcgttg tcacgacatg gattgaaaaa
     3361 attctcactg atttgaaggt ccagcataaa cgagttccct gtggaaaaga ggaagtcagc
     3421 cttttcctaa cggccataga aaactcctgg attcatttaa ggagaaagaa aagagagcgt
     3481 gtgagacagc tgagagaagt tccccgagct cctgaggacg tcattcaggc cttggaagag
     3541 aaaaagccca cccccaaaga gtctggcaat agccaagaac tggccagggg cccccaggag
     3601 aggaccccct gtgggcctgc tctgcgggaa ggcgaggctg ccgctgtgga gggcccgtgc
     3661 ccgagccagg agtccctgtc ccaggaggaa aacccggaac ccacggagga tgaaaggagt
     3721 gaggaaaagg gaggggtgga ggttttggaa agttgtcaag gctctagcaa cggagcccag
     3781 gaccaagagg cttctgagca gttcggcagc ccagtggctg aaagggggaa acgtctccca
     3841 ggagtggccg gacagtacct gtttaagtgt ttgataaacg ttaagaagga ggtggacgat
     3901 gccttagtgg agatgcactg ggttgagggc cagaacaggg atctgatgaa ccagctttgc
     3961 acctacatac gtaaccaaat tttcaggctt gttgcagtta acggatccga ctacaaagac
     4021 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtagcgg
     4081 ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     4141 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc
     4201 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     4261 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     4321 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc
     4381 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc
     4441 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg
     4501 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt
     4561 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga
     4621 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag
     4681 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga
     4741 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg
     4801 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc
     4861 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac
     4921 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac
     4981 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc
     5041 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg
     5101 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac
     5161 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg
     5221 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag
     5281 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc
     5341 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc
     5401 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc
     5461 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     5521 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     5581 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     5641 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     5701 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     5761 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     5821 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     5881 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     5941 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     6001 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa
     6061 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6121 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6181 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     6241 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6301 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6361 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6421 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     6481 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6541 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctccgcctt
     6601 cgccctcaga cgagtcggat ctccctttgg ccgcctcccc gcctggtacc tttaagacca
     6661 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     6721 gggctaattc actcccaacg aaaataagat ctgctttttg cttgtactgg gtctctctgg
     6781 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     6841 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     6901 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     6961 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     7021 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     7081 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     7141 tatcatgtct ggctctagct atcccgcccc taactccgcc cagttccgcc cattctccgc
     7201 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     7261 tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga gacggcccaa
     7321 attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7381 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7441 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7501 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7561 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7621 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7681 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7741 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7801 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7861 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7921 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7981 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8041 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8101 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8161 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8221 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8281 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8341 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8401 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8461 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8521 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8581 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8641 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8701 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8761 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     8821 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8881 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8941 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     9001 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     9061 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     9121 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9181 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9241 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     9301 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9361 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9421 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9481 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     9541 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     9601 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     9661 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     9721 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     9781 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     9841 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     9901 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
