LOCUS       V038165                10654 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038165
VERSION     V038165
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4680
                     /label="MED15(NM_001003891)"
                     /note="MED15(NM_001003891)"
                     /gene="MED15"
     CDS             4687..4752
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4796..5007
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5020..5288
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5317..5326
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5347..6009
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6079..6132
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6133..6732
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6733..7321
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7395..7628
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7700..7821
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7861..7996
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8029..8045)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8053..8069
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8077..8107)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8122..8143
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8431..9019)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9190..10050)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10051..10155)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10629..10645
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg acgtttccgg gcaagagacc
     2341 gactggcgga gcaccgcctt ccggcagaag ctggtcagtc aaatcgagga tgccatgagg
     2401 aaagctggtg tggcacacag taaatccagc aaggatatgg agagccatgt tttcctgaag
     2461 gccaagaccc gggacgaata cctttctctc gtggccaggc tcattatcca ttttcgagac
     2521 attcataaca agaaatctca agcttccgtc agtgatccta tgaatgcact ccagagcctg
     2581 actggcggac ctgctgcggg agccgctgga attggcatgc ctcctcgggg cccgggacag
     2641 tctctgggcg ggatgggtag ccttggtgcc atgggacagc caatgtctct ctcagggcag
     2701 ccgcctcctg ggacctcggg gatggcccct cacagcatgg ctgtcgtgtc tacggcaact
     2761 ccacagaccc agctgcagct ccagcaggtg gcgctgcagc agcagcagca acagcagcag
     2821 ttccagcagc agcagcaggc ggcgctacag cagcagcagc agcagcagca acagcagcag
     2881 ttccaggctc agcagagtgc catgcagcag cagttccaag cagtagtgca gcagcagcag
     2941 cagctccagc agcagcagca gcagcagcag catctaatta aattgcatca tcaaaatcag
     3001 caacagatac agcagcagca acagcagctg cagcgaatag cacagctgca gctccaacaa
     3061 cagcaacagc agcagcagca gcagcagcag cagcagcagc aggctttgca ggcccagcca
     3121 ccaattcagc agccaccgat gcagcagcca cagcctccgc cctcccaggc tctgccccag
     3181 cagctgcagc agatgcatca cacacagcac caccagccgc caccacagcc ccagcagcct
     3241 ccagttgctc agaaccaacc atcacaactc ccgccacagt cgcagaccca gcctttggtg
     3301 tcacaggcgc aagctctccc tggacaaatg ttgtataccc aaccaccact gaaatttgtc
     3361 cgagctccga tggtggtgca gcagccccca gtgcagcccc aggtgcagca gcagcagaca
     3421 gcagtacaga cagctcaggc tgcccagatg gtggctcccg gagtccagat gatcacggaa
     3481 gccttggccc aaggtgggat gcacataaga gcccggttcc cgcctaccac cgctgtgtcc
     3541 gccatcccgt caagctccat ccctttgggc agacagccca tggcacaggt cagccagagc
     3601 agcctcccca tgctgtcctc gccgtcaccg ggccagcagg tgcagacccc gcagtcgatg
     3661 ccccctcccc cccagccgtc cccgcagccc ggccagccca gctcacagcc caactccaac
     3721 gtcagctctg gccctgcccc atctcccagt agcttcctgc ccagcccctc accgcagccc
     3781 tcccagagcc cagtgacggc gcggacccca cagaacttca gtgtcccctc acctggacct
     3841 ttaaacacac ctgtgaaccc cagctctgtc atgagcccag ctggctccag ccaggctgag
     3901 gagcagcagt acctggacaa gctgaagcag ctgtcgaagt acatcgagcc cctgcgccgc
     3961 atgatcaaca agatcgacaa gaacgaagac agaaaaaagg acctgagtaa gatgaagagc
     4021 cttctggaca ttctgacaga cccctcgaag cggtgtcccc tgaagacctt gcaaaagtgt
     4081 gagatcgccc tggagaaact caagaatgac atggcggtgc ccactccccc accgcccccg
     4141 gtgccaccga ccaaacagca gtacctatgc cagccgctcc tggatgccgt cctggccaac
     4201 atccgctcac ctgtcttcaa ccattccctg taccgcacat tcgttccagc catgaccgcc
     4261 attcacggcc cacccatcac ggccccagtg gtgtgcaccc ggaagcgcag gcttgaggat
     4321 gatgagcggc agagcatccc cagtgtgctc cagggtgagg tggccaggct ggaccccaag
     4381 ttcctggtaa acctggaccc ttctcactgc agcaacaatg gcactgtcca cctgatctgc
     4441 aagctggatg acaaggacct cccaagtgtg ccaccactgg agctcagtgt gcccgctgac
     4501 tatcctgccc aaagcccgct gtggatagac cggcagtggc agtacgacgc caaccccttc
     4561 ctccagtcgg tgcaccgctg catgacctcc aggctgctgc agctcccgga caagcactcg
     4621 gtcaccgcct tgctcaacac ctgggcccag agcgtccacc aggcctgcct ctcagccgcc
     4681 ggatccgact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
     4741 gacgatgaca agtagcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca
     4801 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt
     4861 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     4921 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     4981 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca
     5041 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct
     5101 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
     5161 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
     5221 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
     5281 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc
     5341 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc
     5401 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag
     5461 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc
     5521 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac
     5581 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg
     5641 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc
     5701 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg
     5761 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc
     5821 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc
     5881 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag
     5941 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc
     6001 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc
     6061 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag
     6121 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     6181 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     6241 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     6301 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     6361 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     6421 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     6481 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     6541 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     6601 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     6661 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     6721 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     6781 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6841 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     6901 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     6961 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     7021 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     7081 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     7141 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     7201 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     7261 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     7321 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     7381 agaaaagggg ggactggaag ggctaattca ctcccaacga agataagatc tgctttttgc
     7441 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7501 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7561 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7621 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     7681 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     7741 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7801 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     7861 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     7921 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     7981 ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt catagctgtt
     8041 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     8101 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     8161 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     8221 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     8281 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     8341 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8401 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8461 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8521 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8581 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8641 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8701 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8761 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8821 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     8881 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8941 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     9001 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9061 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9121 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9181 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9241 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9301 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9361 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9421 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9481 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9541 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9601 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9661 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9721 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9781 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9841 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9901 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9961 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    10021 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    10081 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    10141 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    10201 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
    10261 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
    10321 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
    10381 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
    10441 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
    10501 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
    10561 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
    10621 acgacgttgt aaaacgacgg ccagtgccaa gctg
//
