LOCUS V036047 12965 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V036047 VERSION V036047 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..3018 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK" /label="EGFP" CDS 3055..7797 /label="MED1(NM_004774)" /note="MED1(NM_004774)" /gene="MED1" CDS 7804..7830 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 7834..7860 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 7864..7890 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 7935..8146 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 8159..8427 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 8454..9053 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 9060..9648 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 9722..9955 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 10027..10148 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 10167..10302 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(10340..10356) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 10364..10380 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(10388..10418) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 10433..10454 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(10742..11330) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(11501..12361) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(12362..12466) /gene="bla" /label="AmpR promoter" primer_bind 12940..12956 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg 2341 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag 2401 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 2461 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc 2521 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac 2581 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 2641 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 2701 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc 2761 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag 2821 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 2881 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac 2941 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 3001 atggacgagc tgtacaaggg cggcggaggg agtggaggag ggggaagtgg atccatgaaa 3061 gctcaggggg aaaccgagga gtcagaaaag ctgagtaaga tgagttctct cctggaacgg 3121 ctccatgcaa aatttaacca aaatagaccc tggagtgaaa ccattaagct tgtgcgtcaa 3181 gtcatggaga agagggttgt gatgagttct ggagggcatc aacatttggt cagctgtttg 3241 gagacattgc agaaggctct caaagtaaca tctttaccag caatgactga tcgtttggag 3301 tccatagcaa gacagaatgg actgggctct catctcagtg ccagtggcac tgaatgttac 3361 atcacgtcag atatgttcta tgtggaagtg cagttagatc ctgcaggaca gctttgtgat 3421 gtaaaagtgg ctcaccatgg ggagaatcct gtgagctgtc cggagcttgt acagcagcta 3481 agggaaaaaa attttgatga attttctaag caccttaagg gccttgttaa tctgtataac 3541 cttccagggg acaacaaact gaagactaaa atgtacttgg ctctccaatc cttagaacaa 3601 gatctttcta aaatggcaat tatgtactgg aaagcaacta atgctggtcc cttggataag 3661 attcttcatg gaagtgttgg ctatctcaca ccaaggagtg ggggtcattt aatgaacctg 3721 aagtactatg tctctccttc tgacctactg gatgacaaga ctgcatctcc catcattttg 3781 catgagaata atgtttctcg atctttgggc atgaatgcat cagtgacaat tgaaggaaca 3841 tctgctgtgt acaaactccc aattgcacca ttaattatgg ggtcacatcc agttgacaat 3901 aaatggaccc cttccttctc ctcaatcacc agtgccaaca gtgttgatct tcctgcctgt 3961 ttcttcttga aatttcccca gccaatccca gtatctagag catttgttca gaaactgcag 4021 aactgcacag gaattccatt gtttgaaact caaccaactt atgcacccct gtatgaactg 4081 atcactcagt ttgagctatc aaaggaccct gaccccatac ctttgaatca caacatgaga 4141 ttttatgctg ctcttcctgg tcagcagcac tgctatttcc tcaacaagga tgctcctctt 4201 ccagatggcc gaagtctaca gggaaccctt gttagcaaaa tcacctttca gcaccctggc 4261 cgagttcctc ttatcctaaa tctgatcaga caccaagtgg cctataacac cctcattgga 4321 agctgtgtca aaagaactat tctgaaagaa gattctcctg ggcttctcca atttgaagtg 4381 tgtcctctct cagagtctcg tttcagcgta tcttttcagc accctgtgaa tgactccctg 4441 gtgtgtgtgg taatggatgt gcaggactca acacatgtga gctgtaaact ctacaaaggg 4501 ctgtcggatg cactgatctg cacagatgac ttcattgcca aagttgttca aagatgtatg 4561 tccatccctg tgacgatgag ggctattcgg aggaaagctg aaaccattca agccgacacc 4621 ccagcactgt ccctcattgc agagacagtt gaagacatgg tgaaaaagaa cctgcccccg 4681 gctagcagcc cagggtatgg catgaccaca ggcaacaacc caatgagtgg taccactaca 4741 ccaaccaaca cctttccggg gggtcccatt accaccttgt ttaatatgag catgagcatc 4801 aaagatcggc atgagtcggt gggccatggg gaggacttca gcaaggtgtc tcagaaccca 4861 attcttacca gtttgttgca aatcacaggg aacggggggt ctaccattgg ctcgagtccg 4921 acccctcctc atcacacgcc gccacctgtc tcttcgatgg ccggcaacac caagaaccac 4981 ccgatgctca tgaaccttct taaagataat cctgcccagg atttctcaac cctttatgga 5041 agcagccctt tagaaaggca gaactcctct tccggctcac cccgcatgga aatatgctcg 5101 gggagcaaca agaccaagaa aaagaagtca tcaagattac cacctgagaa accaaagcac 5161 cagactgaag atgactttca gagggagcta ttttcaatgg atgttgactc acagaaccct 5221 atctttgatg tcaacatgac agctgacacg ctggatacgc cacacatcac tccagctcca 5281 agccagtgta gcactccccc aacaacttac ccacaaccag tacctcaccc ccaacccagt 5341 attcaaagga tggtccgact atccagttca gacagcattg gcccagatgt aactgacatc 5401 ctttcagaca ttgcagaaga agcttctaaa cttcccagca ctagtgatga ttgcccagcc 5461 attggcaccc ctcttcgaga ttcttcaagc tctgggcatt ctcagagtac cctgtttgac 5521 tctgatgtct ttcaaactaa caataatgaa aatccataca ctgatccagc tgatcttatt 5581 gcagatgctg ctggaagccc cagtagtgac tctcctacca atcatttttt tcatgatgga 5641 gtagatttca atcctgattt attgaacagc cagagccaaa gtggttttgg agaagaatat 5701 tttgatgaaa gcagccaaag tggggataat gatgatttca aaggatttgc atctcaggca 5761 ctaaatactt tgggggtgcc aatgcttgga ggtgataatg gggagaccaa gtttaagggc 5821 aataaccaag ccgacacagt tgatttcagt attatttcag tagccggcaa agctttagct 5881 cctgcagatc ttatggagca tcacagtggt agtcagggtc ctttactgac cactggggac 5941 ttagggaaag aaaagactca aaagagggta aaggaaggca atggcaccag taatagtact 6001 ctctcggggc ccggattaga cagcaaacca gggaagcgca gtcggacccc ttctaatgat 6061 gggaaaagca aagataagcc tccaaagcgg aagaaggcag acactgaggg aaagtctcca 6121 tctcatagtt cttctaacag accttttacc ccacctacca gtacaggtgg atctaaatcg 6181 ccaggcagtg caggaagatc tcagactccc ccaggtgttg ccacaccacc cattcccaaa 6241 atcactattc agattcctaa gggaacagtg atggtgggca agccttcctc tcacagtcag 6301 tataccagca gtggttctgt gtcttcctca ggcagcaaaa gccaccatag ccattcttcc 6361 tcctcttcct catctgcttc cacctcaggg aagatgaaaa gcagtaaatc agaaggttca 6421 tcaagttcca agttaagtag cagtatgtat tctagccagg ggtcttctgg atctagccag 6481 tccaaaaatt catcccagtc tggggggaag ccaggctcct ctcccataac caagcatgga 6541 ctgagcagtg gctctagcag caccaagatg aaacctcaag gaaagccatc atcacttatg 6601 aatccttctt taagtaaacc aaacatatcc ccttctcatt caaggccacc tggaggctct 6661 gacaagcttg cctctccaat gaagcctgtt cctggaactc ctccatcctc taaagccaag 6721 tcccctatca gttcaggttc tggtggttct catatgtctg gaactagttc aagctctggc 6781 atgaagtcat cttcagggtt aggatcctca ggctcgttgt cccagaaaac tcccccatca 6841 tctaattcct gtacggcatc ttcctcctcc ttttcctcaa gtggctcttc catgtcatcc 6901 tctcagaacc agcatgggag ttctaaagga aaatctccca gcagaaacaa gaagccgtcc 6961 ttgacagctg tcatagataa actgaagcat ggggttgtca ccagtggccc tgggggtgaa 7021 gacccactgg acggccagat gggggtgagc acaaattctt ccagccatcc tatgtcctcc 7081 aaacataaca tgtcaggagg agagtttcag ggcaagcgtg agaaaagtga taaagacaaa 7141 tcaaaggttt ccacctccgg gagttcagtg gattcttcta agaagacctc agagtcaaaa 7201 aatgtgggga gcacaggtgt ggcaaaaatt atcatcagta agcatgatgg aggctcccct 7261 agcattaaag ccaaagtgac tttgcagaaa cctggggaaa gtagtggaga agggcttagg 7321 cctcaaatgg cttcttctaa aaactatggc tctccactca tcagtggttc cactccaaag 7381 catgagcgtg gctctcccag ccatagtaag tcaccagcat atacccccca gaatctggac 7441 agtgaaagtg agtcaggctc ctccatagca gagaaatctt atcagaatag tcccagctca 7501 gacgatggta tccgaccact tccagaatac agcacagaga aacataagaa gcacaaaaag 7561 gaaaagaaga aagtaaaaga caaagatagg gaccgagacc gggacaaaga ccgagacaag 7621 aaaaaatctc atagcatcaa gccagagagt tggtccaaat cacccatctc ttcagaccag 7681 tccttgtcta tgacaagtaa cacaatctta tctgcagaca gaccctcaag gctcagccca 7741 gactttatga ttggggagga agatgatgat cttatggatg tggccctgat tgggaatgga 7801 tcctacccat acgacgtacc tgattatgca acttatcctt atgatgtacc tgattatgct 7861 agctatcctt atgatgtccc agactacgca taggcggccg cgaaggatct gcgatcgctc 7921 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg 7981 ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt 8041 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt 8101 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg 8161 ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt 8221 gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt 8281 aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag 8341 actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc 8401 gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagatgaccg 8461 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc 8521 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca 8581 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca 8641 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg 8701 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc 8761 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt 8821 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg 8881 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct 8941 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg 9001 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagtcgaca 9061 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 9121 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 9181 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 9241 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 9301 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 9361 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 9421 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg 9481 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 9541 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc 9601 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta 9661 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 9721 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct 9781 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 9841 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 9901 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 9961 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 10021 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 10081 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 10141 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 10201 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 10261 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg 10321 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 10381 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 10441 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 10501 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 10561 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 10621 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 10681 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 10741 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 10801 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 10861 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 10921 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 10981 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 11041 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 11101 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 11161 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 11221 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 11281 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 11341 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 11401 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 11461 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 11521 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 11581 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 11641 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 11701 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 11761 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 11821 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 11881 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 11941 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 12001 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 12061 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 12121 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 12181 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 12241 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 12301 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 12361 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 12421 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 12481 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 12541 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 12601 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 12661 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 12721 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 12781 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 12841 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 12901 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca 12961 agctg //