LOCUS       V022802                12997 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022802
VERSION     V022802
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..7039
                     /label="MED1(NM_004774)"
                     /note="MED1(NM_004774)"
                     /gene="MED1"
     CDS             7046..7111
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        7156..7367
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7380..7648
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7677..7686
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7707..8369
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             8439..8492
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8493..9092
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    9093..9680
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9754..9987
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    10059..10180
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      10199..10334
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10372..10388)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10396..10412
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10420..10450)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10465..10486
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10774..11362)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11533..12393)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12394..12498)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12972..12988
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatga aagctcaggg ggaaaccgag gagtcagaaa agctgagtaa
     2341 gatgagttct ctcctggaac ggctccatgc aaaatttaac caaaatagac cctggagtga
     2401 aaccattaag cttgtgcgtc aagtcatgga gaagagggtt gtgatgagtt ctggagggca
     2461 tcaacatttg gtcagctgtt tggagacatt gcagaaggct ctcaaagtaa catctttacc
     2521 agcaatgact gatcgtttgg agtccatagc aagacagaat ggactgggct ctcatctcag
     2581 tgccagtggc actgaatgtt acatcacgtc agatatgttc tatgtggaag tgcagttaga
     2641 tcctgcagga cagctttgtg atgtaaaagt ggctcaccat ggggagaatc ctgtgagctg
     2701 tccggagctt gtacagcagc taagggaaaa aaattttgat gaattttcta agcaccttaa
     2761 gggccttgtt aatctgtata accttccagg ggacaacaaa ctgaagacta aaatgtactt
     2821 ggctctccaa tccttagaac aagatctttc taaaatggca attatgtact ggaaagcaac
     2881 taatgctggt cccttggata agattcttca tggaagtgtt ggctatctca caccaaggag
     2941 tgggggtcat ttaatgaacc tgaagtacta tgtctctcct tctgacctac tggatgacaa
     3001 gactgcatct cccatcattt tgcatgagaa taatgtttct cgatctttgg gcatgaatgc
     3061 atcagtgaca attgaaggaa catctgctgt gtacaaactc ccaattgcac cattaattat
     3121 ggggtcacat ccagttgaca ataaatggac cccttccttc tcctcaatca ccagtgccaa
     3181 cagtgttgat cttcctgcct gtttcttctt gaaatttccc cagccaatcc cagtatctag
     3241 agcatttgtt cagaaactgc agaactgcac aggaattcca ttgtttgaaa ctcaaccaac
     3301 ttatgcaccc ctgtatgaac tgatcactca gtttgagcta tcaaaggacc ctgaccccat
     3361 acctttgaat cacaacatga gattttatgc tgctcttcct ggtcagcagc actgctattt
     3421 cctcaacaag gatgctcctc ttccagatgg ccgaagtcta cagggaaccc ttgttagcaa
     3481 aatcaccttt cagcaccctg gccgagttcc tcttatccta aatctgatca gacaccaagt
     3541 ggcctataac accctcattg gaagctgtgt caaaagaact attctgaaag aagattctcc
     3601 tgggcttctc caatttgaag tgtgtcctct ctcagagtct cgtttcagcg tatcttttca
     3661 gcaccctgtg aatgactccc tggtgtgtgt ggtaatggat gtgcaggact caacacatgt
     3721 gagctgtaaa ctctacaaag ggctgtcgga tgcactgatc tgcacagatg acttcattgc
     3781 caaagttgtt caaagatgta tgtccatccc tgtgacgatg agggctattc ggaggaaagc
     3841 tgaaaccatt caagccgaca ccccagcact gtccctcatt gcagagacag ttgaagacat
     3901 ggtgaaaaag aacctgcccc cggctagcag cccagggtat ggcatgacca caggcaacaa
     3961 cccaatgagt ggtaccacta caccaaccaa cacctttccg gggggtccca ttaccacctt
     4021 gtttaatatg agcatgagca tcaaagatcg gcatgagtcg gtgggccatg gggaggactt
     4081 cagcaaggtg tctcagaacc caattcttac cagtttgttg caaatcacag ggaacggggg
     4141 gtctaccatt ggctcgagtc cgacccctcc tcatcacacg ccgccacctg tctcttcgat
     4201 ggccggcaac accaagaacc acccgatgct catgaacctt cttaaagata atcctgccca
     4261 ggatttctca accctttatg gaagcagccc tttagaaagg cagaactcct cttccggctc
     4321 accccgcatg gaaatatgct cggggagcaa caagaccaag aaaaagaagt catcaagatt
     4381 accacctgag aaaccaaagc accagactga agatgacttt cagagggagc tattttcaat
     4441 ggatgttgac tcacagaacc ctatctttga tgtcaacatg acagctgaca cgctggatac
     4501 gccacacatc actccagctc caagccagtg tagcactccc ccaacaactt acccacaacc
     4561 agtacctcac ccccaaccca gtattcaaag gatggtccga ctatccagtt cagacagcat
     4621 tggcccagat gtaactgaca tcctttcaga cattgcagaa gaagcttcta aacttcccag
     4681 cactagtgat gattgcccag ccattggcac ccctcttcga gattcttcaa gctctgggca
     4741 ttctcagagt accctgtttg actctgatgt ctttcaaact aacaataatg aaaatccata
     4801 cactgatcca gctgatctta ttgcagatgc tgctggaagc cccagtagtg actctcctac
     4861 caatcatttt tttcatgatg gagtagattt caatcctgat ttattgaaca gccagagcca
     4921 aagtggtttt ggagaagaat attttgatga aagcagccaa agtggggata atgatgattt
     4981 caaaggattt gcatctcagg cactaaatac tttgggggtg ccaatgcttg gaggtgataa
     5041 tggggagacc aagtttaagg gcaataacca agccgacaca gttgatttca gtattatttc
     5101 agtagccggc aaagctttag ctcctgcaga tcttatggag catcacagtg gtagtcaggg
     5161 tcctttactg accactgggg acttagggaa agaaaagact caaaagaggg taaaggaagg
     5221 caatggcacc agtaatagta ctctctcggg gcccggatta gacagcaaac cagggaagcg
     5281 cagtcggacc ccttctaatg atgggaaaag caaagataag cctccaaagc ggaagaaggc
     5341 agacactgag ggaaagtctc catctcatag ttcttctaac agacctttta ccccacctac
     5401 cagtacaggt ggatctaaat cgccaggcag tgcaggaaga tctcagactc ccccaggtgt
     5461 tgccacacca cccattccca aaatcactat tcagattcct aagggaacag tgatggtggg
     5521 caagccttcc tctcacagtc agtataccag cagtggttct gtgtcttcct caggcagcaa
     5581 aagccaccat agccattctt cctcctcttc ctcatctgct tccacctcag ggaagatgaa
     5641 aagcagtaaa tcagaaggtt catcaagttc caagttaagt agcagtatgt attctagcca
     5701 ggggtcttct ggatctagcc agtccaaaaa ttcatcccag tctgggggga agccaggctc
     5761 ctctcccata accaagcatg gactgagcag tggctctagc agcaccaaga tgaaacctca
     5821 aggaaagcca tcatcactta tgaatccttc tttaagtaaa ccaaacatat ccccttctca
     5881 ttcaaggcca cctggaggct ctgacaagct tgcctctcca atgaagcctg ttcctggaac
     5941 tcctccatcc tctaaagcca agtcccctat cagttcaggt tctggtggtt ctcatatgtc
     6001 tggaactagt tcaagctctg gcatgaagtc atcttcaggg ttaggatcct caggctcgtt
     6061 gtcccagaaa actcccccat catctaattc ctgtacggca tcttcctcct ccttttcctc
     6121 aagtggctct tccatgtcat cctctcagaa ccagcatggg agttctaaag gaaaatctcc
     6181 cagcagaaac aagaagccgt ccttgacagc tgtcatagat aaactgaagc atggggttgt
     6241 caccagtggc cctgggggtg aagacccact ggacggccag atgggggtga gcacaaattc
     6301 ttccagccat cctatgtcct ccaaacataa catgtcagga ggagagtttc agggcaagcg
     6361 tgagaaaagt gataaagaca aatcaaaggt ttccacctcc gggagttcag tggattcttc
     6421 taagaagacc tcagagtcaa aaaatgtggg gagcacaggt gtggcaaaaa ttatcatcag
     6481 taagcatgat ggaggctccc ctagcattaa agccaaagtg actttgcaga aacctgggga
     6541 aagtagtgga gaagggctta ggcctcaaat ggcttcttct aaaaactatg gctctccact
     6601 catcagtggt tccactccaa agcatgagcg tggctctccc agccatagta agtcaccagc
     6661 atataccccc cagaatctgg acagtgaaag tgagtcaggc tcctccatag cagagaaatc
     6721 ttatcagaat agtcccagct cagacgatgg tatccgacca cttccagaat acagcacaga
     6781 gaaacataag aagcacaaaa aggaaaagaa gaaagtaaaa gacaaagata gggaccgaga
     6841 ccgggacaaa gaccgagaca agaaaaaatc tcatagcatc aagccagaga gttggtccaa
     6901 atcacccatc tcttcagacc agtccttgtc tatgacaagt aacacaatct tatctgcaga
     6961 cagaccctca aggctcagcc cagactttat gattggggag gaagatgatg atcttatgga
     7021 tgtggccctg attgggaatg gatccgacta caaagaccat gacggtgatt ataaagatca
     7081 tgacatcgac tacaaggatg acgatgacaa gtgagcggcc gcgaaggatc tgcgatcgct
     7141 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     7201 gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     7261 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     7321 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag
     7381 gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt
     7441 tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg
     7501 taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta
     7561 gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt
     7621 cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg ctagacgcca
     7681 ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc atcaccggca
     7741 ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc aagcagggcc
     7801 gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc tacctgctga
     7861 gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc tacgagaacc
     7921 ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag aagtacgagg
     7981 acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc gtgatcggcg
     8041 acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc gacaagatca
     8101 tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg ctggtgggca
     8161 gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc
     8221 acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc cccatgttcg
     8281 ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg gagtaccagc
     8341 acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg
     8401 tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga agtcttctaa
     8461 catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc acggtgcgcc
     8521 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     8581 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     8641 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     8701 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     8761 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     8821 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     8881 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     8941 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     9001 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     9061 cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac aaaatttgtg
     9121 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     9181 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     9241 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     9301 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     9361 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     9421 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     9481 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     9541 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     9601 tgctgccggc tctgcggcct cttccgcgtc tccgccttcg ccctcagacg agtcggatct
     9661 ccctttggcc gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag
     9721 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     9781 aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     9841 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     9901 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     9961 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
    10021 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
    10081 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    10141 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
    10201 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
    10261 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
    10321 cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat ggtcatagct
    10381 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
    10441 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
    10501 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
    10561 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
    10621 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
    10681 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
    10741 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    10801 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    10861 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    10921 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    10981 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    11041 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    11101 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    11161 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
    11221 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    11281 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    11341 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    11401 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    11461 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    11521 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    11581 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    11641 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    11701 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    11761 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    11821 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    11881 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    11941 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    12001 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    12061 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    12121 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    12181 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    12241 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    12301 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    12361 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    12421 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    12481 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
    12541 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
    12601 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
    12661 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
    12721 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
    12781 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc
    12841 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
    12901 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
    12961 gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
