LOCUS       V014325                 8189 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014325
VERSION     V014325
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8189)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        50..429
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        430..633
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             683..2869
                     /gene="TBK1"
                     /label="Serine/threonine-protein kinase TBK1"
                     /note="Serine/threonine-protein kinase TBK1 from Homo
                     sapiens. Accession#: Q9UHD2"
     CDS             2876..2941
                     /label="3xFLAG"
                     /note="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
     misc_feature    2965..3553
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    3619..3667
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3869..4297
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4311..4640
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4707..5498
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5677..5810
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5847..5863)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5871..5887)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5895..5925)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5940..5961)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6249..6837)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7011..7868)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7869..7973)
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccgcca ccatgcagag cacttctaat catctttggc ttttatctga
      721 tatcttgggc caaggagcta ctgcaaatgt ttttcgcgga agacataaga aaactggtga
      781 cttatttgct atcaaagtat ttaataacat aagcttcctt cgtccagtgg atgttcaaat
      841 gagagaattt gaagtgttaa aaaaactcaa tcacaaaaat attgtcaaat tatttgctat
      901 tgaagaggag acaacaacaa gacataaagt acttataatg gaattttgtc catgtgggag
      961 tttatacact gttttagaag aaccatctaa tgcctatgga ctaccagagt ctgaattttt
     1021 aattgttttg agagatgtgg tgggtggaat gaatcatctc cgagaaaatg gcatagtcca
     1081 ccgtgatatc aagccaggaa atatcatgcg tgtgataggg gaagatggac agtctgtgta
     1141 caaactcaca gattttggtg cagccagaga attagaagat gatgagcagt ttgtttctct
     1201 gtatggcaca gaagaatatt tgcatcctga tatgtatgag agagctgtgc taagaaaaga
     1261 tcatcagaaa aaatatggag caacagttga tctttggagc attggggtaa cattttacca
     1321 tgcagctact ggatcactgc catttaggcc atttgaaggg ccacgtagga ataaggaagt
     1381 gatgtataaa ataattactg gaaagccttc tggtgccata tctggagtac agaaggcaga
     1441 gaacgggcca attgactgga gtggagacat gcctgtttct tgtagccttt ctcggggtct
     1501 tcaagttctt cttacccctg ttcttgcaaa catccttgag gcggatcagg aaaagtgttg
     1561 gggttttgac cagttttttg cagaaactag tgatatactt caccgaatga taattcatgt
     1621 tttttctcta caacaaatga ctgctcataa gatttatatt catagctata atactgctac
     1681 tgtgtttcat gaactggtgt ataaacaaac caaaattatt tcttcaaatc aagaacttat
     1741 atatgaagga cgacgtttag ttctagaacc tggaagactg gcacagcact tccctgaaac
     1801 tactgaggaa aatcccatct ttgtagtaag ccgggaacct ctgagtacca taggattgat
     1861 atatgaaaaa atttccctcc ctaaagtgca tccacgttat gatttagatg gggatgccag
     1921 cgtggctaag gcaataacag gaattgtgtg ttatgcctgt agaattgcca gtaccttgct
     1981 gctttatcag gaattaatgc gaaaggggat acgatggttg atagaattag ttaaagatga
     2041 ttataatgaa actgttcaca aaaagacaga agttgtgatc acattggatt tctacatcag
     2101 aaacattgaa aaaactgtga cagtatatga aaagttgatg aagatcaacc tagaaacaac
     2161 agagttaggt gaaatttcag acatacacac caaattgtta agactttcca gttctcaggg
     2221 aacaatagaa accagtcttc aggatattga aagcaaatta tctccaggtg gattgctggc
     2281 tgatacatgg acccatcaag aaggcactca tccaaaagac agacatgtag aaaagctaca
     2341 agtcctgtta aattgcatca cagagattta ctatcaattc aaaaaagaca aagcagaacg
     2401 tagactagct tataatgaag aacaaatcca caaatttgac aagcaaaaac tattttatca
     2461 tgcaacaaaa gctatgaccc actttacaga agaatgtgtt aaaaagtatg aggcattttt
     2521 ggataagtca gaagaatgga tgagaaagat gctgcatctc aggaaacagt tactatccct
     2581 aactaaccag tgttttgata ttgaagaaga agtgtcaaag tatcaagact acactaatga
     2641 gttacaagaa acattgccac agaaaatgtt tgcagcctcc agtggaatca aacataccat
     2701 gaccccaatt tatccatgtt ctaacacatt agtagaaatg actcttggta tgaagaaatt
     2761 gaaggaagaa atggaagggg tagttaaaga acttgccgaa aataatcaca ttttagaaag
     2821 gtttggctct ttaaccatgg atggtggcct tcgcaatgtt gactgtcttc tcgaggacta
     2881 caaagaccat gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa
     2941 gtagtgaggg cccgatatct cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     3001 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     3061 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3121 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     3181 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     3241 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     3301 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     3361 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     3421 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     3481 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     3541 gccgcctccc cgcctggaaa cgggggaggc taactgaaac acggaaggag acaataccgg
     3601 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggg tgttgggtcg
     3661 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
     3721 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
     3781 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcagatctg
     3841 cgcagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     3901 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     3961 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4021 cggggcatcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     4081 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     4141 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4201 cctatctcgg tctattcttt tgatttataa gggattttgg ggatttcggc ctattggtta
     4261 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     4321 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4381 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4441 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     4501 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     4561 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     4621 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     4681 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     4741 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     4801 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     4861 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     4921 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     4981 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     5041 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     5101 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     5161 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     5221 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     5281 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     5341 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     5401 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     5461 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgc
     5521 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     5581 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     5641 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     5701 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     5761 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     5821 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     5881 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     5941 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6001 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     6061 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     6121 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     6181 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6241 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6301 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6361 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     6421 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc
     6481 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     6541 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     6601 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     6661 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     6721 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     6781 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     6841 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     6901 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     6961 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7021 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7081 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7141 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     7201 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7261 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7321 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7381 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     7441 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     7501 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     7561 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     7621 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     7681 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     7741 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     7801 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     7861 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     7921 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     7981 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtcga
     8041 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     8101 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
     8161 ccgacaattg catgaagaat ctgcttagg
//
