LOCUS       V014973                10821 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014973
VERSION     V014973
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10821)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2338..3519
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             3548..5539
                     /gene="SLC5A1"
                     /label="Sodium/glucose cotransporter 1"
                     /note="Sodium/glucose cotransporter 1 from Homo sapiens.
                     Accession#: P13866"
     misc_feature    5571..6157
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             6177..6773
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    6790..7378
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             7585..8218
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8394..8424)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8439..8460)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8748..9336)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9510..10367)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10368..10472)
                     /label="AmpR promoter"
     polyA_signal    10520..10654
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtacgaatt
     3541 cgccaccatg gacagtagca cctggagccc caagaccacc gcggtcaccc ggcctgttga
     3601 gacccacgag ctcattcgca atgcagccga tatctccatc atcgttatct acttcgtggt
     3661 agtgatggcc gtcggactgt gggctatgtt ttccaccaat cgtgggactg ttggaggctt
     3721 cttcctggca ggccgaagta tggtgtggtg gccgattgga gcctccctct ttgctagtaa
     3781 cattggaagt ggccactttg tggggctggc cgggactggg gcagcttcag gcatcgccat
     3841 tggaggcttt gaatggaatg ccctggtttt ggtggttgtg ctgggctggc tgtttgtccc
     3901 catctatatt aaggctgggg tggtgacaat gccagagtac ctgaggaagc ggtttggagg
     3961 ccagcggatc caggtctacc tttcccttct gtccctgctg ctctacattt tcaccaagat
     4021 ctcggcagac atcttctcgg gggccatatt catcaatctg gccttaggcc tgaatctgta
     4081 tttagccatc tttctcttat tggcaatcac tgccctttac acaattacag ggggcctggc
     4141 ggcggtgatt tacacggaca ccttgcagac ggtgatcatg ctggtggggt ctttaatcct
     4201 gactgggttt gcttttcacg aagtgggagg ctatgacgcc ttcatggaaa agtacatgaa
     4261 agccattcca accatagtgt ctgatggcaa caccaccttt caggaaaaat gctacactcc
     4321 aagggccgac tccttccaca tcttccgaga tcccctcacg ggagacctcc catggcctgg
     4381 gttcatcttt gggatgtcca tccttacctt gtggtactgg tgcacagatc aggtcattgt
     4441 gcagcgctgc ctctcagcca agaatatgtc tcacgtgaag ggtggctgca tcctgtgtgg
     4501 gtatctaaag ctgatgccca tgttcatcat ggtgatgcca ggaatgatca gccgcattct
     4561 gtacacagaa aaaattgcct gtgtcgtccc ttcagaatgt gagaaatatt gcggtaccaa
     4621 ggttggctgt accaacatcg cctatccaac cttagtggtg gagctcatgc ccaatggact
     4681 gcgaggcctg atgctatcag tcatgctggc ctccctcatg agctccctga cctccatctt
     4741 caacagcgcc agcaccctct tcaccatgga catctacgcc aaggtccgca agagagcatc
     4801 tgagaaagag ctcatgattg ccggaaggtt gtttatcctg gtgctgattg gcatcagcat
     4861 cgcctgggtg cccattgtgc agtcagcaca aagtgggcaa ctcttcgatt acatccagtc
     4921 catcaccagt tacttgggac cacccattgc ggctgtcttc ctgcttgcta ttttctggaa
     4981 gagagtcaat gagccaggag ccttttgggg actgatccta ggacttctga ttgggatttc
     5041 acgtatgatt actgagtttg cttatggaac cgggagctgc atggagccca gcaactgtcc
     5101 cacgattatc tgtggggtgc actacttgta ctttgccatt atcctcttcg ccatttcttt
     5161 catcaccatc gtggtcatct ccctcctcac caaacccatt ccggatgtgc atctctaccg
     5221 tctgtgttgg agcctgcgca acagcaaaga ggagcgtatt gacctggatg cggaagagga
     5281 gaacatccaa gaaggcccta aggagaccat tgaaatagaa acacaagttc ctgagaagaa
     5341 aaaaggaatc ttcaggagag cctatgacct attttgtggg ctagagcagc acggtgcacc
     5401 caagatgact gaggaagagg agaaagccat gaagatgaag atgacggaca cctctgagaa
     5461 gcctttgtgg aggacagtgt tgaacgtcaa tggcatcatc ctggtgaccg tggctgtctt
     5521 ttgccatgca tattttgcct gaactagttc tagagcggcc gcggatcccg cccctctccc
     5581 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     5641 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     5701 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     5761 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     5821 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     5881 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     5941 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     6001 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     6061 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     6121 ttcctttgaa aaacacgatg ataagcttgc cacaacccac aaggagacga ccttccatga
     6181 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca
     6241 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc
     6301 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     6361 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     6421 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     6481 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     6541 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     6601 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     6661 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     6721 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctagacgc
     6781 gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     6841 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6901 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     6961 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     7021 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     7081 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     7141 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     7201 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     7261 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     7321 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     7381 ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt agcaatacag
     7441 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
     7501 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7561 actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagata
     7621 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     7681 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     7741 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     7801 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     7861 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     7921 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     7981 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     8041 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     8101 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     8161 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     8221 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     8281 agtgagaggc cttgacattg ctagcgttta ccgtcgacct ctagctagag cttggcgtaa
     8341 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     8401 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     8461 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     8521 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     8581 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     8641 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     8701 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     8761 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     8821 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     8881 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     8941 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     9001 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     9061 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     9121 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     9181 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     9241 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     9301 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     9361 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     9421 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     9481 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     9541 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     9601 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     9661 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     9721 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     9781 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     9841 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     9901 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     9961 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    10021 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    10081 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    10141 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    10201 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    10261 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    10321 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    10381 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    10441 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
    10501 gtcgacggat cgggagatca acttgtttat tgcagcttat aatggttaca aataaagcaa
    10561 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    10621 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
    10681 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
    10741 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
    10801 atgtactaca aacttagtag t
//
