LOCUS       V027812                10067 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027812
VERSION     V027812
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4239
                     /label="MCAM(NM_006500)"
                     /note="MCAM(NM_006500)"
                     /gene="MCAM"
     CDS             4276..4986
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="enhanced monomeric blue fluorescent protein
                     (Subach et al., 2011)"
                     /transl_table=1
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
                     /label="mTagBFP2"
     promoter        5037..5248
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5261..5529
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5556..6155
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6162..6750
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6824..7057
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7129..7250
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7269..7404
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7442..7458)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7466..7482
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7490..7520)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7535..7556
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7844..8432)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8603..9463)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9464..9568)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10042..10058
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggggctt cccaggctgg tctgcgcctt cttgctcgcc
     2341 gcctgctgct gctgtcctcg cgtcgcgggt gtgcccggag aggctgagca gcctgcgcct
     2401 gagctggtgg aggtggaagt gggcagcaca gcccttctga agtgcggcct ctcccagtcc
     2461 caaggcaacc tcagccatgt cgactggttt tctgtccaca aggagaagcg gacgctcatc
     2521 ttccgtgtgc gccagggcca gggccagagc gaacctgggg agtacgagca gcggctcagc
     2581 ctccaggaca gaggggctac tctggccctg actcaagtca ccccccaaga cgagcgcatc
     2641 ttcttgtgcc agggcaagcg ccctcggtcc caggagtacc gcatccagct ccgcgtctac
     2701 aaagctccgg aggagccaaa catccaggtc aaccccctgg gcatccctgt gaacagtaag
     2761 gagcctgagg aggtcgctac ctgtgtaggg aggaacgggt accccattcc tcaagtcatc
     2821 tggtacaaga atggccggcc tctgaaggag gagaagaacc gggtccacat tcagtcgtcc
     2881 cagactgtgg agtcgagtgg tttgtacacc ttgcagagta ttctgaaggc acagctggtt
     2941 aaagaagaca aagatgccca gttttactgt gagctcaact accggctgcc cagtgggaac
     3001 cacatgaagg agtccaggga agtcaccgtc cctgttttct acccgacaga aaaagtgtgg
     3061 ctggaagtgg agcccgtggg aatgctgaag gaaggggacc gcgtggaaat caggtgtttg
     3121 gctgatggca accctccacc acacttcagc atcagcaagc agaaccccag caccagggag
     3181 gcagaggaag agacaaccaa cgacaacggg gtcctggtgc tggagcctgc ccggaaggaa
     3241 cacagtgggc gctatgaatg tcagggcctg gacttggaca ccatgatatc gctgctgagt
     3301 gaaccacagg aactactggt gaactatgtg tctgacgtcc gagtgagtcc cgcagcccct
     3361 gagagacagg aaggcagcag cctcaccctg acctgtgagg cagagagtag ccaggacctc
     3421 gagttccagt ggctgagaga agagacaggc caggtgctgg aaagggggcc tgtgcttcag
     3481 ttgcatgacc tgaaacggga ggcaggaggc ggctatcgct gcgtggcgtc tgtgcccagc
     3541 atacccggcc tgaaccgcac acagctggtc aacgtggcca tttttggccc cccttggatg
     3601 gcattcaagg agaggaaggt gtgggtgaaa gagaatatgg tgttgaatct gtcttgtgaa
     3661 gcgtcagggc acccccggcc caccatctcc tggaacgtca acggcacggc aagtgaacaa
     3721 gaccaagatc cacagcgagt cctgagcacc ctgaatgtcc tcgtgacccc ggagctgttg
     3781 gagacaggtg ttgaatgcac ggcctccaac gacctgggca aaaacaccag catcctcttc
     3841 ctggagctgg tcaatttaac caccctcaca ccagactcca acacaaccac tggcctcagc
     3901 acttccactg ccagtcctca taccagagcc aacagcacct ccacagagag aaagctgccg
     3961 gagccggaga gccggggcgt ggtcatcgtg gctgtgattg tgtgcatcct ggtcctggcg
     4021 gtgctgggcg ctgtcctcta tttcctctat aagaagggca agctgccgtg caggcgctca
     4081 gggaagcagg agatcacgct acccccgtct cgtaagagcg aacttgtagt tgaagttaag
     4141 tcagataagc tcccagaaga gatgggcctc ctgcagggca gcagcggtga caagagggct
     4201 ccgggagacc agggagagaa atacatcgat ctgaggcatg gtggaggcgg atcaggaggc
     4261 ggtggatcgc tcgagatggt gtctaagggc gaagagctga ttaaggagaa catgcacatg
     4321 aagctgtaca tggagggcac cgtggacaac catcacttca agtgcacatc cgagggcgaa
     4381 ggcaagccct acgagggcac ccagaccatg agaatcaagg tggtcgaggg cggccctctc
     4441 cccttcgcct tcgacatcct ggctactagc ttcctctacg gcagcaagac cttcatcaac
     4501 cacacccagg gcatccccga cttcttcaag cagtccttcc ctgagggctt cacatgggag
     4561 agagtcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac cagcctccag
     4621 gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcacatc caacggccct
     4681 gtgatgcaga agaaaacact cggctgggag gccttcaccg agacgctgta ccccgctgac
     4741 ggcggcctgg aaggcagaaa cgacatggcc ctgaagctcg tgggcgggag ccatctgatc
     4801 gcaaacgcca agaccacata tagatccaag aaacccgcta agaacctcaa gatgcctggc
     4861 gtctactatg tggactacag actggaaaga atcaaggagg ccaacaacga gacctacgtc
     4921 gagcagcacg aggtggcagt ggccagatac tgcgacctcc ctagcaaact ggggcacaag
     4981 cttaattaag gatccgcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc
     5041 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg
     5101 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     5161 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     5221 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc
     5281 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc
     5341 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc
     5401 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac
     5461 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg
     5521 ttacagatcc aagctgtgac cggcgcctac gctagatgac cgagtacaag cccacggtgc
     5581 gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg
     5641 actaccccgc cacgcgccac accgtcgacc cggaccgcca catcgagcgg gtcaccgagc
     5701 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     5761 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     5821 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     5881 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     5941 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     6001 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     6061 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     6121 gcacctggtg catgacccgc aagcccggtg cctgagtcga caatcaacct ctggattaca
     6181 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     6241 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     6301 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     6361 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     6421 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     6481 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     6541 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     6601 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     6661 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     6721 gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat gacttacaag
     6781 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     6841 tcccaacgaa aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     6901 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6961 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7021 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     7081 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     7141 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7201 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     7261 ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     7321 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     7381 agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat
     7441 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     7501 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     7561 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     7621 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7681 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7741 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7801 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7861 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7921 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7981 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8041 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8101 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8161 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8221 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8281 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8341 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8401 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8461 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8521 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8581 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8641 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8701 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8761 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8821 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8881 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8941 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     9001 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     9061 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9121 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     9181 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     9241 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9301 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9361 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9421 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9481 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9541 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     9601 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     9661 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     9721 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     9781 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     9841 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     9901 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     9961 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
    10021 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
