LOCUS       V038415                11279 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038415
VERSION     V038415
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5364
                     /label="MAML1(NM_014757)"
                     /note="MAML1(NM_014757)"
                     /gene="MAML1"
     CDS             5371..5394
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHHHH"
                     /label="8xHis"
     promoter        5438..5649
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5662..5930
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5959..5968
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5989..6651
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6721..6774
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6775..7374
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7375..7962
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8036..8269
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8341..8462
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8481..8616
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8654..8670)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8678..8694
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8702..8732)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8747..8768
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9056..9644)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9815..10675)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10676..10780)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11254..11270
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg tgctgcccac ctgccccatg
     2341 gcggagttcg cgctgccgcg gcacagcgcg gtcatggagc gccttcgccg gcgcatcgag
     2401 ctgtgccggc gccaccacag cacctgcgag gcccgctacg aggccgtgtc gcccgagcgc
     2461 ctggagctgg agcgccaaca caccttcgcc ctgcaccagc gctgcatcca ggccaaggcc
     2521 aagcgcgccg ggaagcacag gcagccgccc gccgccacgg ccccggcgcc cgccgccccg
     2581 gccccgcgcc tggacgccgc tgacggcccc gagcacggcc gcccggccac gcatcttcat
     2641 gatacagtta agaggaatct tgacagcgcc acttcccctc agaatggcga tcaacagaat
     2701 ggctacgggg acctctttcc tgggcataag aagactcgcc gggaggcccc tctgggagtt
     2761 gccatctctt ccaatggact gcctccagcc tcccccctcg gtcagtctga caagccttct
     2821 ggagccgacg ccctgcagtc cagtgggaag cactctctgg ggctagactc tctcaacaaa
     2881 aagcgtctgg ctgactccag ccttcacttg aatggaggca gtaaccccag tgagtcattt
     2941 cctctgagcc tgaataaaga actgaagcag gagcctgtcg aagacctgcc ttgcatgatc
     3001 actgggactg tcggctccat atcgcaaagc aacctcatgc cagacctcaa ccttaacgag
     3061 caggagtgga aggagctcat cgaggagctg aacaggtcgg tgcccgatga agacatgaag
     3121 gacctgttta atgaggactt cgaggagaag aaggacccag agtcttctgg ctctgccaca
     3181 caaaccccct tggcacagga cattaatatt aagacggaat tctctccagc agcctttgag
     3241 caagaacagt taggctctcc acaagtgagg gccgggtctg cagggcagac ctttctgggg
     3301 ccttcctctg cccctgtgag tacagattcc cccagcctag ggggctccca aaccttattc
     3361 cacacctctg gtcagccccg ggcggacaat cccagtccaa acctgatgcc ggcatcagcc
     3421 caggcccaga acgcacaaag agcccttgca ggtgtggtat tgcccagtca gggcccagga
     3481 ggggcctcag agctgtcctc tgcccaccag ctccagcaga tcgctgccaa gcagaagcgc
     3541 gagcagatgc tccagaaccc acagcaggcc accccggcac cagccccggg ccagatgtcc
     3601 acatggcagc agacggggcc ctcccacagt tccttagatg tcccttaccc catggagaag
     3661 cctgccagcc cttccagcta caagcaagac ttcactaact ccaaactgct catgatgcct
     3721 agtgtgaata agagttcccc tcggcccgga ggcccctacc tccagcccag ccatgtgaac
     3781 ctgctgagtc accagccacc gagtaacttg aatcagaact ccgcgaataa ccaggggtct
     3841 gtgctggact acggcaatac aaaacccctt tctcattaca aagcggactg tgggcaaggc
     3901 agcccggggt ctggccagag caagccagcc ctgatggctt atcttcccca gcagctgtcc
     3961 catataagtc acgagcagaa ctccctgttt ctgatgaagc caaagccagg aaatatgcct
     4021 ttccgatcac tggttccacc tggccaggag cagaaccctt ccagtgtccc tgtgcaagcc
     4081 caggctacca gtgttgggac ccagccgcct gccgtgtccg tggccagctc ccacaacagc
     4141 tccccctatc tcagcagcca gcaacaggcc gctgtaatga agcagcatca gttgcttttg
     4201 gaccaacaga aacaaaggga gcagcagcaa aagcatttac agcaacagca gttccttcag
     4261 aggcaacagc accttctcgc ggaacaggag aagcaacagt ttcagcgcca tctgacccgc
     4321 ccaccacccc agtaccaaga cccgacacaa ggcagcttcc cacagcaggt tggacagttc
     4381 acagggtcct ctgctgccgt gcccggcatg aacaccttgg gtccatccaa ctccagctgt
     4441 cctcgagtgt tccctcaggc tgggaatctg atgccaatgg gccctggaca tgcttcagtt
     4501 tcctctctcc ccacaaactc aggccaacag gaccggggtg tggctcagtt ccctggctcc
     4561 caaaacatgc ctcagagcag cctctatggc atggcttctg gcataaccca gatagttgcc
     4621 cagcccccgc cacaggccac caatggacat gcccacattc cacggcagac caacgtgggc
     4681 cagaacacct ccgtctcagc tgcctatggg cagaactctc tgggaagctc tggcctctcc
     4741 cagcagcaca ataaggggac cctgaaccct ggtttaacaa agccaccggt cccaagggtg
     4801 tcaccagcca tgggaggcca gaattcctcc tggcagcatc agggaatgcc gaacctcagt
     4861 ggccagaccc cagggaacag caacgtgagt cccttcactg cagcctccag tttccacatg
     4921 cagcagcagg cccacctgaa aatgtctagc ccgcaattct cccaggcagt gcccaacagg
     4981 cccatggctc ccatgagctc agcagctgcc gtggggtcct tgctaccccc agtgagtgca
     5041 cagcagagga ccagcgcccc tgccccagca ccacccccaa cagcccctca gcagggcttg
     5101 cctggcctga gcccagcagg gcctgagctg ggggccttca gccagagccc tgcctcacag
     5161 atgggcggtc gggcggggct gcactgcacc caggcctacc ctgtgcggac cgcgggccag
     5221 gagctgcctt ttgcctatag cgggcagcca ggtggcagtg ggctctctag tgtggctgga
     5281 cacaccgatc tgatcgactc cctgctgaag aacaggactt cagaggagtg gatgagtgat
     5341 ttggacgacc tgttagggtc tcagggatcc caccatcacc atcaccatca ccattagcgg
     5401 ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     5461 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc
     5521 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     5581 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     5641 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc
     5701 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc
     5761 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg
     5821 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt
     5881 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga
     5941 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag
     6001 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga
     6061 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg
     6121 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc
     6181 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac
     6241 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac
     6301 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc
     6361 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg
     6421 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac
     6481 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg
     6541 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag
     6601 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc
     6661 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc
     6721 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc
     6781 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     6841 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     6901 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     6961 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     7021 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     7081 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     7141 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     7201 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     7261 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     7321 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa
     7381 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     7441 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     7501 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     7561 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     7621 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     7681 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     7741 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     7801 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     7861 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctccgcctt
     7921 cgccctcaga cgagtcggat ctccctttgg ccgcctcccc gcctggtacc tttaagacca
     7981 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     8041 gggctaattc actcccaacg aaaataagat ctgctttttg cttgtactgg gtctctctgg
     8101 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     8161 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     8221 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     8281 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     8341 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8401 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     8461 tatcatgtct ggctctagct atcccgcccc taactccgcc cagttccgcc cattctccgc
     8521 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     8581 tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga gacggcccaa
     8641 attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     8701 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     8761 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     8821 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     8881 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     8941 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     9001 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     9061 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     9121 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     9181 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     9241 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     9301 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     9361 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     9421 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     9481 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     9541 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     9601 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     9661 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     9721 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     9781 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     9841 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     9901 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     9961 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    10021 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    10081 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    10141 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    10201 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    10261 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    10321 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    10381 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    10441 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    10501 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    10561 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    10621 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    10681 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    10741 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    10801 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
    10861 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
    10921 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
    10981 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
    11041 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
    11101 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
    11161 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
    11221 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
