LOCUS       V028913                10963 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028913
VERSION     V028913
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4989
                     /label="Lpin1(NM_172950)"
                     /note="Lpin1(NM_172950)"
                     /gene="Lpin1"
     CDS             4996..5061
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5105..5316
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5329..5597
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5626..5635
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5656..6318
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6388..6441
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6442..7041
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7042..7630
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7704..7937
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8009..8130
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8170..8305
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8338..8354)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8362..8378
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8386..8416)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8431..8452
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8740..9328)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9499..10359)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10360..10464)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10938..10954
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga attacgtggg gcagctggcc
     2341 ggccaggtgt ttgtgacggt gaaggagctc tacaagggac tgaaccccgc cacgctgtcg
     2401 ggatgcatcg acatcattgt catccggcag cccaatggca gtctgcagtg ctcccctttc
     2461 cacgtccgct tcggcaagat gggtgtcctc cgctcccgag agaaagtggt ggacatagaa
     2521 atcaatgggg agtccgtgga tttgcacatg aagttgggag acaacggaga agcatttttt
     2581 gttcaagaga ctgacaacga tcaggaaatc atccccatgt acctggccac gtcccccatc
     2641 ctgtcagaag gagctgcgag aatggaaagc cagctgaaga ggaactctgt agacagaatc
     2701 aggtgcctgg atcccactac agctgcccag ggcctgcctc ccagcgacac cccatccact
     2761 ggttctctgg ggaagaagag aaggaaaagg aggaggaagg cccagttgga caatctcaaa
     2821 agagatgaca atgtcaacac atctgaggat gaggacatgt ttcccataga gatgagctcg
     2881 gatgaggaca cagcaccgat ggatggaagc agaactcttc ctaatgatgt accaccattc
     2941 caagatgata ttcctaagga aaacttcccc tcgatttcaa cgtaccccca gtcagcatcg
     3001 tatcccagtt cggacagaga atggtccccc agccccagtc cttcaggctc ccggccctca
     3061 acaccaaaaa gtgactctga gctggtcagt aagtctgcag acaggttgac gccaaagaat
     3121 aacctggaaa tgctctggct gtggggtgaa ttgccacagg ctgcaaagtc ctcttctcca
     3181 cacaagatga aagagtccag ccccttaggg agccggaaga ctcctgataa aatgaatttt
     3241 caggccattc acagcgagtc ttcagatact tttagtgacc agtcgccaac aatggcccgg
     3301 ggactgctca tccaccagag taaggcccag acggaaatgc agtttgtgaa cgaggaggat
     3361 ctcgagtcct tgggggcggc agccccacct tcacccgtgg ccgaagagct caaggcccca
     3421 tatcccaaca ccgcacagtc gtcgagcaag acagattccc cttccaggaa gaaagataaa
     3481 cggagccgac accttggagc tgatggtgtt tatctggacg acctcacgga catggaccct
     3541 gaagtggcag ccctgtattt ccccaagaat ggggatcctg gtgggctccc caaacaagcc
     3601 agtgacaacg gagccaggtc agccaaccag tcaccacagt ctgtgggagg ctcgggcatc
     3661 gacagtggtg tggagagcac ctccgacagc ctgagggacc tgccatccat cgccatctcc
     3721 ctctgcggtg gcctcagtga ccacagagag atcaccaaag atgcattttt ggaacaagcc
     3781 gtgtcatatc agcaatttgc cgacaaccct gctatcatcg atgaccccaa cctcgtggtc
     3841 aaggttggca ataagtatta caactggaca acagcagctc ctctacttct ggcgatgcag
     3901 gctttccaga aacctttgcc aaaggccact gtggaatcca tcatgagaga taagatgccc
     3961 aaaaagggag gaagatggtg gttttcctgg agaggaagaa atgccacaat caaagaggaa
     4021 agcaagcctg aacagtgcct gactgggaaa ggccacaata ccggagagca gcctgcccag
     4081 cttggcctgg ccaccaggat aaagcatgag tcatcctcca gtgatgaaga gcacgcagcc
     4141 gccaagccat caggttcgag ccacctctct ctcttgtcca acgtcagcta caaaaagacc
     4201 ctgcggctca cgtcggagca gctgaaaagc ttgaagttga agaacggccc caatgatgtg
     4261 gtgttcagtg tcactaccca gtaccagggc acctgtcgct gcgagggcac catctacctg
     4321 tggaattggg acgacaaagt catcatctca gatatcgatg ggaccatcac aagatctgat
     4381 actcttggtc acattttgcc cacgctggga aaggattgga ctcaccaggg cattgcaaag
     4441 ctgtaccaca aagtaagcca gaatggctac aagtttctct attgttcggc acgtgccatt
     4501 gggatggcgg acatgacgag gggctacctg cactgggtca acgagagggg cacggtgctt
     4561 ccacagggcc cgcttctgct cagcccgagc agcctcttct ccgccttgca cagagaagtg
     4621 attgaaaaga agccagaaaa gttcaaagtc cagtgtttga cagacatcaa gaacctgttt
     4681 ttcccaaaca cagaaccctt ctatgctgct tttgggaacc ggcctgctga tgtgtattcc
     4741 tacaagcaag tgggagtgtc cctgaatagg atcttcactg tgaaccccaa gggtgagctg
     4801 gtgcaggagc atgccaagac caacatcagc tcgtacgtgc ggctctgcga agtggtcgat
     4861 cacgtcttcc cattgctaaa gagaagccat tcctgtgact tcccctgttc agacactttc
     4921 agtaacttca ccttttggag agagccactg ccaccttttg aaaaccagga catgcattca
     4981 gcctcagctg gatccgacta caaagaccat gacggtgatt ataaagatca tgacatcgac
     5041 tacaaggatg acgatgacaa gtagcggccg cgaaggatct gcgatcgctc cggtgcccgt
     5101 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     5161 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     5221 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     5281 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
     5341 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
     5401 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
     5461 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
     5521 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
     5581 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggagagc
     5641 gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc
     5701 gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac
     5761 aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg
     5821 ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac
     5881 gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg
     5941 ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg
     6001 gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac
     6061 gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc
     6121 accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc
     6181 aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc
     6241 gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag
     6301 acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc
     6361 gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac
     6421 gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc
     6481 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     6541 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     6601 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     6661 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     6721 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     6781 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     6841 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     6901 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     6961 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     7021 acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga aagattgact
     7081 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     7141 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     7201 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7261 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     7321 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     7381 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     7441 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     7501 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     7561 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     7621 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     7681 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gataagatct
     7741 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7801 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7861 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7921 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     7981 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     8041 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     8101 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     8161 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8221 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     8281 tagtgaggag gcttttttgg aggcctagac ttttgcagag accaaattcg taatcatgtc
     8341 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     8401 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     8461 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     8521 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     8581 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8641 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8701 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8761 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8821 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8881 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     8941 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     9001 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9061 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9121 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9181 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9241 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9301 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9361 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9421 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9481 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9541 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9601 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9661 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9721 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9781 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9841 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9901 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9961 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    10021 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    10081 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    10141 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    10201 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10261 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10321 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10381 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10441 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
    10501 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
    10561 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
    10621 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
    10681 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
    10741 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
    10801 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
    10861 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
    10921 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
