LOCUS       V024556                11309 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024556
VERSION     V024556
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5352
                     /label="Lig3(NM_001291247)"
                     /note="Lig3(NM_001291247)"
                     /gene="Lig3"
     CDS             5359..5424
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5468..5679
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5692..5960
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5989..5998
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6019..6681
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6751..6804
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6805..7404
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7405..7992
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8066..8299
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8371..8492
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8511..8646
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8684..8700)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8708..8724
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8732..8762)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8777..8798
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9086..9674)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9845..10705)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10706..10810)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11284..11300
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga ctttggcttt caagatcctc
     2341 ttcccgagaa acctttgtgc ccttggcaga aaagaactgt gcctgttccc agaacagaat
     2401 cgctgggctg ccataagtca gttcagccag tggtcggaaa caaatctcct cggtgggtgc
     2461 tgccttctcc agagaagaaa gcctgtccta gctctccaga gaggtcatct aagaccacgt
     2521 gccacccacc ttactttctg gccagggtcg catgtgggac tctgtactgg cccctgtgcg
     2581 atggcagagc agcggttctg tgtggactat gccaagcggg gcacagctgg atgcaagaaa
     2641 tgcaaggaga agattgtaaa gggcgtgtgc cgcattggca aagtggtgcc caaccccttc
     2701 tcagagtctg ggggtgatat gaaagagtgg taccacatta agtgcatgtt tgagaaactg
     2761 gaacgggctc gggccaccac aaagaaaatt gaagacctca cagagctgga aggctgggaa
     2821 gagctggaag ataacgaaaa ggaacagata agccagcata ttgcagacct gtcttctaag
     2881 gcagctgcca cacctaagaa gaaagctgct gtccaggcta agctgacaac cactggccag
     2941 gtgacatctc cagtgaaagg tgcttcgttt attaccagta ccaatcctcg gaagttctct
     3001 ggcttttcag cagcaaaacc caacaactct gagcaagccc cctcaagccc tgctcctgga
     3061 acgagtctgt ctgcaagtaa atgtgaccct aagcacaaag attgtctgct acgagagttc
     3121 cggaagctgt gcgccatggt ggctgaaaat cctagctata acaccaagac ccagatcatc
     3181 catgacttct tgcagaaagg ctccacagga gatggctttc acggtgatgt gtacctgaca
     3241 gttaagctgc tgctgcctgg tgtcatcaag agtgtttaca acttgaatga taagcagatt
     3301 gtaaaacttt ttagccgaat ttttaactgc aacccagatg atatggcccg ggacctagaa
     3361 cagggtgacg tgtcagagac gatcagaatc ttctttgagc agagcaagtc tttcccccca
     3421 gctgccaaaa gcctcctcac cattcaggaa gtggatgcct tcctcctgca cctctccaag
     3481 ctcaccaaag aggatgagca gcagcaggcc ctgcaggaca tagcctcccg gtgtacagcc
     3541 aatgacctta agtgcattat tcggctgatc aaacatgatc tgaagatgaa ctcgggtgca
     3601 aaacacgtgt tagatgccct tgaccccaat gcttatgaag ccttcaaagc atcgcgcaac
     3661 ctgcaggatg tggtggagcg agtccttcac aatgagcagg aggtggagaa ggacccaggc
     3721 cggcgacggg ctctgagggt ccaggcctca ctgatgacgc ctgtgcagcc catgctggct
     3781 gaggcctgca aatccattga atatgcaatg aagaagtgtc ccaatggcat gttctctgag
     3841 atcaagtacg atggtgagcg agtccaggtg cataagaagg gggaccactt cagctacttc
     3901 agccgcagtc tcaagcctgt cctgcctcac aaggtggccc acttcaagga ctatataccc
     3961 aaagcttttc ctgggggtca aagcatgatc ttggattccg aggtactcct gattgacaac
     4021 aacacaggca aaccactgcc ctttgggact ctgggagtgc acaagaaagc tgctttccag
     4081 gatgctaatg tctgcctgtt tgtttttgat tgtatctact ttaatgatgt cagcttgatg
     4141 gacaggcctc tctgtgagcg acggaagttt cttcatgaca acatggttga aatccgtaac
     4201 cggatcatgt tctcagaaat gaagcaagtc acaaaagctt cagacctggc tgacatgata
     4261 aaccgggtga tccgagaggg cttggaaggg ttggtgctaa aggatgtgaa gggtacatat
     4321 gagcctggga agcggcactg gctgaaagtt aagaaagatt atttgaatga gggggccatg
     4381 gctgatacag ctgatctggt ggttcttggg gccttctatg ggcaagggag caaaggcgga
     4441 atgatgtcca tcttcctcat gggctgctat gaccctgaca gccaaaagtg gtgcactgtt
     4501 acaaaatgtg ctggaggcca tgatgatgcc acacttgccc gcctgcagaa agagctggtt
     4561 atggtgaaga tcagcaagga tcctagtaag atacccagct ggctgaagat caacaagatc
     4621 tattatcctg actttatcgt cccagaccca aagaaagctg ctgtgtggga gatcacaggg
     4681 gcagaattct ccagatctga ggcacacact gctgatggca tctccatccg atttcctcga
     4741 tgcacccgaa tccgggacga caaggactgg aaatctgcca ctaacctccc ccaactcaag
     4801 gaactgtacc agctgtccaa agacaaggca gactttgctg tggtggctgg agatgaggca
     4861 agccccacta ctggaggcag cagtggcgag aacgagggca ctgctgggtc tgctgggccc
     4921 tgcaagggcc cccccagtaa gtcctctgcc agtgccaaga ccaccgaaca gaagctcaat
     4981 agccccagta gcagaggtgg catcaaaccg attccaaagc attcccccat gaaaccagga
     5041 gagaagctgg ctgtgaagtc ttctccagtc aaagtgggaa tgaagaggaa agctactgat
     5101 gagaccccat gcctgaaaaa ggtactgttg gatgtcttca ctggggtgcg gctctacttg
     5161 ccaccttcta caccagactt caaacgtctc aaacgctact ttgtggcatt cgacggggac
     5221 ctggtacagg aatttgacat gggctcagcc acacatgtgc taggtaacag ggaaaagaac
     5281 actgatgccc agttggtctc ctcagagtgg atttgggcat gtatccggaa acggaggctg
     5341 atagctccct gcggatccga ctacaaagac catgacggtg attataaaga tcatgacatc
     5401 gactacaagg atgacgatga caagtagcgg ccgcgaagga tctgcgatcg ctccggtgcc
     5461 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     5521 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     5581 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     5641 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc
     5701 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
     5761 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
     5821 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
     5881 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
     5941 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag
     6001 agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac
     6061 ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc
     6121 aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg
     6181 atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg
     6241 cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc
     6301 gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag
     6361 gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc
     6421 aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc
     6481 cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac
     6541 ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc
     6601 cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc
     6661 aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc
     6721 accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt
     6781 gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc
     6841 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     6901 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     6961 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     7021 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     7081 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     7141 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     7201 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     7261 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     7321 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     7381 atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg
     7441 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     7501 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     7561 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     7621 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     7681 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     7741 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     7801 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     7861 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     7921 gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat ctccctttgg
     7981 ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     8041 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat
     8101 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     8161 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     8221 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     8281 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     8341 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     8401 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     8461 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     8521 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     8581 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     8641 gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg
     8701 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     8761 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     8821 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     8881 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     8941 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     9001 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9061 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9121 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9181 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9241 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     9301 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9361 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     9421 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     9481 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     9541 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9601 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     9661 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     9721 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     9781 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     9841 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     9901 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     9961 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    10021 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    10081 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    10141 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    10201 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    10261 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    10321 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    10381 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    10441 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    10501 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    10561 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    10621 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    10681 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    10741 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    10801 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
    10861 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
    10921 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
    10981 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
    11041 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
    11101 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
    11161 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
    11221 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
    11281 gttgtaaaac gacggccagt gccaagctg
//
