LOCUS       V038537                11566 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038537
VERSION     V038537
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5592
                     /label="Lifr(NM_013584)"
                     /note="Lifr(NM_013584)"
                     /gene="Lifr"
     CDS             5599..5664
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5708..5919
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5932..6200
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6229..6238
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6259..6921
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6991..7044
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7045..7644
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7645..8233
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8307..8540
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8612..8733
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8773..8908
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8941..8957)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8965..8981
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8989..9019)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9034..9055
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9343..9931)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10102..10962)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10963..11067)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11541..11557
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cagcttactc atggtggaga
     2341 cagccatcgt ggatggtaga caataaaaga tcgaggatga ctccaaacct gccatggctc
     2401 ctgtcagctc tgaccctcct gcatctgacg atgcatgcaa acggtctgaa gagaggggta
     2461 caagacttga aatgcacaac caacaacatg cgagtgtggg actgcacgtg gccagctccc
     2521 ctcggggtca gccctggaac tgttaaagat atttgcatta aagacaggtt ccattcttgt
     2581 cacccattag agacaacaaa cgttaaaatt ccagctcttt cacctggtga tcacgaagtc
     2641 acaataaatt atctaaatgg ctttcagagt aaattcacgt tgaatgaaaa agatgtctct
     2701 ttaattccag agactcccga gatcctggat ttgtctgctg acttcttcac ctcctcctta
     2761 ctactgaagt ggaacgacag agggtctgct ctgcctcacc cctccaatgc cacctgggag
     2821 attaaggttc tacagaatcc aaggacggaa ccagtagcac tcgtgttact caacacaatg
     2881 ctgagtggta aagataccgt tcagcactgg aactggacct cagacctgcc cttgcaatgt
     2941 gccactcact cggtgagcat tcgatggcac attgactcgc ctcatttctc cggttacaaa
     3001 gagtggagtg actggagccc gctgaagaac atctcctgga ttcgtaatac agagactaat
     3061 gtttttcctc aagacaaagt ggtgctcgca ggctcaaaca tgacaatttg ttgtatgagt
     3121 ccaacgaaag tgctttcagg acagatcggc aatacccttc gtcctctcat ccatctgtac
     3181 gggcaaaccg ttgcgatcca tatcctgaac atccccgttt ctgaaaacag tggcacaaac
     3241 atcattttca tcacagacga cgatgtgtac ggaacggtgg tctttgcagg ctatcctccc
     3301 gatgttcctc agaagctgag ctgtgagaca catgacttaa aagagattat atgtagctgg
     3361 aatccaggaa ggataactgg actggtgggc ccacgaaata cagaatacac cctgtttgaa
     3421 agcatttcag gaaaatcggc agtatttcac aggattgaag gacttacaaa cgagacctac
     3481 cggttaggcg tgcaaatgca tcccggccaa gaaatccata acttcaccct gactggtcgc
     3541 aatccactgg ggcaggcaca gtcagcagtg gtcatcaatg tgactgagag agttgctcct
     3601 catgatccga cttcgttgaa agtgaaggac atcaattcaa cagttgttac attttcttgg
     3661 tatttaccag gaaattttac aaagattaat cttttatgtc aaattgaaat ttgtaaagct
     3721 aattccaaga aagaagtgag gaatgccaca atcagaggag ccgaggattc aacttaccat
     3781 gttgctgtag acaaattaaa tccatacact gcatacactt tccgggttcg ttgttcttcc
     3841 aagactttct ggaagtggag caggtggagt gatgagaagc gacatctaac cacagaagcc
     3901 actccttcaa agggaccaga cacttggaga gagtggagtt ctgatggaaa aaatctaatc
     3961 gtctactgga agcctttacc tattaatgaa gctaatggaa aaatactttc ctacaatgtt
     4021 tcgtgttcat tgaacgagga gacacagtca gttttggaga tcttcgatcc tcaacacaga
     4081 gcagagatac agcttagtaa aaatgactac atcatcagtg tggtggcaag aaattctgct
     4141 ggctcatcac caccttcgaa aatagctagt atggaaatcc caaatgacga catcacagta
     4201 gagcaagcgg tggggctagg aaacaggatc ttcctcacct ggcgtcacga ccccaacatg
     4261 acttgtgact acgtaattaa atggtgcaac tcatctcggt ctgagccctg cctcctggac
     4321 tggagaaagg ttccttcaaa cagcacggag actgtcatag agtctgatca gtttcagcca
     4381 ggagtaagat acaactttta cctctatggg tgcactaacc agggatacca actgttacgt
     4441 tccataattg gatacgtaga agaactggct cccattgtcg cgccaaattt caccgtggaa
     4501 gatacgtcgg cagactcgat attagtgaaa tgggacgaca tccccgtgga agagctccga
     4561 ggcttcctaa gagggtattt attttacttt cagaaaggag agagagatac gcccaagacg
     4621 aggagcttgg agccacatca ttctgacatc aagctaaaga acatcactga catatcccag
     4681 aagacactga ggattgctga ccttcagggt aaaaccagtt accatctggt cctgcgagcc
     4741 tatacacatg gtggtctggg cccagagaag agcatgtttg tggtgaccaa ggaaaactct
     4801 gtgggattga ttattgccat cctcatccca gtggctgtgg ctgtcattgt tggcgtggta
     4861 acgagcatcc tttgctatcg gaagcgagaa tggattaagg aaacattcta cccagatatt
     4921 cccaatccag aaaactgtaa ggcgctacag tttcagaaga gcgtctgcga gggaagcaat
     4981 gctcttaaga cattggaaat gaacccctgt accccgaaca acgttgaagt cctggaatct
     5041 cggtcgatag tccctaaaat agaagataca gaaataattt ccccagttgc tgagcgtcct
     5101 ggggaaagat ctgaggtgga ccctgagaac catgtggtcg tgtcttactg cccacccatc
     5161 atcgaggagg aaataacgaa ccccgcggcc gatgaagtgg gaggggcttc ccaggtcgtg
     5221 tacatcgatg tgcagtccat gtatcagccg caagccaaag cagaggaaga gcaggacgtg
     5281 gatcctgtgg tggtggcagg ctataagcca cagatgcgcc ttcccatcag ccccgctgtg
     5341 gaagacacag cagcagaaga tgaagagggt aagaccgccg gttacagacc tcaggccaat
     5401 gtaaacactt ggaatttggt ctctccagat tccccgaggt ccacagacag caacaacgaa
     5461 gttgtctctt ttggaagtcc gtgctccatc aattccaggc aattcttgat tcctcctaaa
     5521 gacgaagact ctcctaaatc taatggagga gggtggtcct ttacaaactt cttccagaac
     5581 aaaccaaatg acggatccga ctacaaagac catgacggtg attataaaga tcatgacatc
     5641 gactacaagg atgacgatga caagtagcgg ccgcgaagga tctgcgatcg ctccggtgcc
     5701 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     5761 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     5821 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     5881 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc
     5941 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
     6001 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
     6061 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
     6121 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
     6181 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag
     6241 agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac
     6301 ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc
     6361 aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg
     6421 atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg
     6481 cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc
     6541 gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag
     6601 gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc
     6661 aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc
     6721 cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac
     6781 ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc
     6841 cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc
     6901 aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc
     6961 accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt
     7021 gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc
     7081 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     7141 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     7201 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     7261 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     7321 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     7381 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     7441 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     7501 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     7561 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     7621 atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg
     7681 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     7741 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     7801 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     7861 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     7921 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     7981 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     8041 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     8101 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     8161 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     8221 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     8281 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagataaga
     8341 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     8401 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     8461 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     8521 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     8581 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     8641 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8701 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     8761 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     8821 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     8881 aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat tcgtaatcat
     8941 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     9001 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     9061 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     9121 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     9181 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9241 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9301 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9361 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9421 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9481 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9541 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9601 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9661 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9721 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     9781 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     9841 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     9901 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     9961 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    10021 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    10081 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    10141 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10201 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10261 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10321 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10381 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    10441 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    10501 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10561 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10621 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10681 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10741 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    10801 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    10861 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10921 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    10981 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    11041 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
    11101 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    11161 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
    11221 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    11281 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
    11341 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    11401 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    11461 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    11521 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
