LOCUS       .                       9311 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        6..233
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    458..583
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1076..1309
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    1804..1920
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2010..2213
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2302..4425
                     /label="large T antigen"
                     /note="large T antigen from SV40 (simian virus 40)"
     promoter        4476..4687
                     /label="EF-1α core promoter"
                     /note="core promoter for human elongation factor EF-1α"
     LTR             4700..4968
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5001..5396
                     /label="BSD"
                     /note="blasticidin S deaminase"
     misc_feature    5406..5994
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6068..6301
                     /label="3' LTR (ΔU3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6373..6507
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6513..6648
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     primer_bind     complement(6686..6702)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6710..6726)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6734..6764)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6779..6800)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7088..7676)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7850..8707)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(8708..8812)
                     /label="AmpR promoter"
     primer_bind     9286..9302
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggataaa gttttaaaca gagaggaatc tttgcagcta
     2341 atggaccttc taggtcttga aaggagtgcc tgggggaata ttcctctgat gagaaaggca
     2401 tatttaaaaa aatgcaagga gtttcatcct gataaaggag gagatgaaga aaaaatgaag
     2461 aaaatgaata ctctgtacaa gaaaatggaa gatggagtaa aatatgctca tcaacctgac
     2521 tttggaggct tctgggatgc aactgagatt ccaacctatg gaactgatga atgggagcag
     2581 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
     2641 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
     2701 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
     2761 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
     2821 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
     2881 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
     2941 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
     3001 agtgccttga ctagagatcc attttctgtt attgaggaaa gtttgccagg tgggttaaag
     3061 gagcatgatt ttaatccaga agaagcagag gaaactaaac aagtgtcctg gaagcttgta
     3121 acagagtatg caatggaaac aaaatgtgat gatgtgttgt tattgcttgg gatgtacttg
     3181 gaatttcagt acagttttga aatgtgttta aaatgtatta aaaaagaaca gcccagccac
     3241 tataagtacc atgaaaagca ttatgcaaat gctgctatat ttgctgacag caaaaaccaa
     3301 aaaaccatat gccaacaggc tgttgatact gttttagcta aaaagcgggt tgatagccta
     3361 caattaacta gagaacaaat gttaacaaac agatttaatg atcttttgga taggatggat
     3421 ataatgtttg gttctacagg ctctgctgac atagaagaat ggatggctgg agttgcttgg
     3481 ctacactgtt tgttgcccaa aatggattca gtggtgtatg actttttaaa atgcatggtg
     3541 tacaacattc ctaaaaaaag atactggctg tttaaaggac caattgatag tggtaaaact
     3601 acattagcag ctgctttgct tgaattatgt ggggggaaag ctttaaatgt taatttgccc
     3661 ttggacaggc tgaactttga gctaggagta gctattgacc agtttttagt agtttttgag
     3721 gatgtaaagg gcactggagg ggagtccaga gatttgcctt caggtcaggg aattaataac
     3781 ctggacaatt taagggatta tttggatggc agtgttaagg taaacttaga aaagaaacac
     3841 ctaaataaaa gaactcaaat atttccccct ggaatagtca ccatgaatga gtacagtgtg
     3901 cctaaaacac tgcaggccag atttgtaaaa caaatagatt ttaggcccaa agattattta
     3961 aagcattgcc tggaacgcag tgagtttttg ttagaaaaga gaataattca aagtggcatt
     4021 gctttgcttc ttatgttaat ttggtacaga cctgtggctg agtttgctca aagtattcag
     4081 agcagaattg tggagtggaa agagagattg gacaaagagt ttagtttgtc agtgtatcaa
     4141 aaaatgaagt ttaatgtggc tatgggaatt ggagttttag attggctaag aaacagtgat
     4201 gatgatgatg aagacagcca ggaaaatgct gataaaaatg aagatggtgg ggagaagaac
     4261 atggaagact cagggcatga aacaggcatt gattcacagt cccaaggctc atttcaggcc
     4321 cctcagtcct cacagtctgt tcatgatcat aatcagccat accacatttg tagaggtttt
     4381 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataagg atccgcggcc
     4441 gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     4501 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg
     4561 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     4621 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     4681 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg
     4741 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg
     4801 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc
     4861 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc
     4921 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc
     4981 ggcgcctacg ctaggccacc atggccaagc ctttgtctca agaagaatcc accctcattg
     5041 aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg
     5101 cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg
     5161 gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga
     5221 cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc
     5281 gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac
     5341 agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag
     5401 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     5461 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     5521 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     5581 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     5641 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     5701 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     5761 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     5821 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     5881 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     5941 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggta
     6001 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     6061 gggggactgg aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact
     6121 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6181 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6241 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6301 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     6361 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     6421 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     6481 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg
     6541 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     6601 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca
     6661 gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     6721 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     6781 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     6841 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6901 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     6961 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7021 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7081 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7141 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7201 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7261 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7321 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7381 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7441 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7501 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     7561 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7621 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7681 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7741 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7801 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7861 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7921 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     7981 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8041 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8101 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8161 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8221 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8281 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8341 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8401 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8461 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     8521 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     8581 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     8641 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     8701 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8761 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     8821 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     8881 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     8941 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     9001 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     9061 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     9121 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
     9181 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     9241 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     9301 gtgccaagct g
//
