LOCUS       V031666                13753 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031666
VERSION     V031666
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..7779
                     /label="LTBP2(NM_000428)"
                     /note="LTBP2(NM_000428)"
                     /gene="LTBP2"
     CDS             7786..7851
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        7895..8106
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             8119..8387
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      8416..8425
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             8446..9108
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             9178..9231
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             9232..9831
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    9832..10420
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             10494..10727
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    10799..10920
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      10960..11095
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(11128..11144)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11152..11168
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11176..11206)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11221..11242
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11530..12118)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12289..13149)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13150..13254)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     13728..13744
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga ggccgcggac caaagcccgc
     2341 agcccggggc gcgccctgcg gaacccctgg agaggcttcc tgccgctcac cctggctctc
     2401 ttcgtgggcg cgggtcatgc ccaaagggac cccgtaggga gatacgagcc ggctggtgga
     2461 gacgcgaatc gactgcggcg ccctgggggc agctacccgg cagcggctgc agccaaggtg
     2521 tacagtctgt tccgggagca ggacgcgcct gtcgcgggct tgcagcccgt ggagcgggcc
     2581 cagccgggct gggggagccc caggaggccc accgaggcgg aggccaggag gccgtcccgc
     2641 gcgcagcagt cgcggcgtgt ccagccacct gcgcagaccc ggagaagcac tcccctgggc
     2701 cagcagcaac cagcaccccg gacccgggcc gcgccggctc tcccacgcct ggggacccca
     2761 cagcggtctg gggctgcgcc cccaaccccg ccgcgagggc ggctcacggg gaggaacgtc
     2821 tgcgggggac agtgctgccc aggatggaca acagcaaaca gcaccaacca ctgtatcaaa
     2881 cccgtttgcg agccgccgtg ccagaaccgg ggctcctgca gccgcccgca gctctgtgtc
     2941 tgccgctctg gtttccgtgg agcccgctgc gaggaggtca ttcccgatga ggaatttgac
     3001 ccccagaact ccaggctggc acctcgacgc tgggccgagc gttcacccaa cctgcgcagg
     3061 agcagtgcgg ctggagaggg caccttggcc agagcacagc cgccagcacc acagtcgccg
     3121 cccgcaccac agtcgccacc agctgggacc ctgagtggcc tcagccagac ccacccttcc
     3181 cagcagcacg tggggttgtc ccgcactgtc cgacttcacc cgactgccac ggccagtagc
     3241 cagctctctt ccaacgccct gcccccggga ccaggccttg agcagagaga tggcacccaa
     3301 caggcggtac ctctggagca cccctcatcc ccctgggggc tgaacctcac ggagaaaatc
     3361 aagaagatca agatcgtctt cactcccacc atctgcaagc agacctgtgc ccgtggacac
     3421 tgtgccaaca gctgtgagag gggcgacacc accaccctgt acagccaggg cggccatggg
     3481 cacgatccca agtctggctt ccgcatctat ttctgccaga tcccctgcct gaacggaggc
     3541 cgctgcatcg gcagggacga atgctggtgc cccgccaact ccaccgggaa gttctgccac
     3601 ctgcctatcc cgcagccgga cagggagcct ccagggaggg ggtcccgccc cagggccttg
     3661 ctggaagccc cactgaagca gtccactttc acactgccgc tctccaacca gctggcctcc
     3721 gtgaacccct ccctggtgaa ggtgcacatt caccacccac ccgaggcctc agtgcagatc
     3781 caccaggtgg cccaggtgcg gggcggggtg gaggaggccc tagtggagaa cagcgtggag
     3841 accagacccc cgccctggct gcctgccagc cctggccaca gcctctggga cagcaacaac
     3901 atccctgctc ggtctggaga gccccctcgg ccactgcccc cagcagcacc caggcctcga
     3961 ggactgctgg gccggtgtta cctgaacact gtgaacggac agtgtgccaa ccctctgctg
     4021 gagctgacta cccaggagga ctgctgtggc agtgtgggag ccttctgggg ggtgactttg
     4081 tgtgccccat gcccacccag accagcctcc ccggtgattg agaatggcca gctggagtgt
     4141 cctcaggggt acaagagact gaacctcact cactgccaag atatcaacga gtgcttgacc
     4201 ctgggcctgt gcaaggacgc ggagtgtgtg aataccaggg gcagctacct gtgcacatgc
     4261 agacctggcc tcatgctgga tccatcgcgg agccgctgtg tgtcggacaa ggcaatctcc
     4321 atgctgcagg gactgtgcta ccggtcgctg gggcccggca cctgcaccct gcctttggcc
     4381 cagcggatca ccaagcagat atgctgctgc agccgcgtgg gcaaagcatg gggcagcgag
     4441 tgtgagaaat gccctctgcc tggcacagag gccttcagag agatctgccc tgccggccac
     4501 ggctacacct acgcgagctc cgacatccgc ctgtccatga ggaaagccga ggaggaggaa
     4561 ctggcaaggc ccccaaggga gcaagggcag aggagcagcg gggcactgcc cgggccagca
     4621 gagaggcagc ccctccgggt cgtcacggac acctggcttg aggccgggac catccctgac
     4681 aagggtgact ctcaggctgg ccaggtcacg accagtgtca ctcatgcacc tgcctgggtc
     4741 acagggaatg ccacaacccc accaatgcct gaacagggga ttgcagagat acaggaagaa
     4801 caagtgaccc cctccactga tgtgctggtg accctgagca ccccaggcat tgacagatgc
     4861 gctgctggag ccaccaacgt ctgtggccct ggaacctgcg tgaacctccc cgatggatac
     4921 agatgtgtct gcagccctgg ctaccagctg caccccagcc aggcctactg cacagatgac
     4981 aacgagtgtc tgagggaccc ctgcaaggga aaagggcgct gcatcaaccg cgtggggtcc
     5041 tactcctgct tctgctaccc tggctacact ctggccacct caggggcgac acaggagtgt
     5101 caagatatca atgagtgtga gcagccaggg gtgtgcagcg gggggcagtg caccaacacc
     5161 gagggctcgt accactgcga gtgtgatcag ggctacatca tggtcaggaa aggacactgc
     5221 caagatatca acgaatgccg tcaccccggt acctgccctg atgggagatg cgtcaattcc
     5281 cctggctcct acacttgtct ggcctgtgag gagggctacc ggggccagag tgggagctgt
     5341 gtagatgtga atgagtgtct gactcccggg gtctgtgccc atggaaagtg caccaaccta
     5401 gaaggctcct tcagatgctc ttgtgagcag ggctatgagg tcacctcaga tgagaagggc
     5461 tgccaagatg tggatgagtg tgccagccgg gcctcatgcc ccacaggcct ctgcctcaac
     5521 acggagggct ccttcgcctg ctctgcctgt gagaacgggt actgggtgaa tgaagacggc
     5581 actgcctgtg aagacctaga tgagtgtgcc ttcccgggag tctgcccctc cggagtctgc
     5641 accaacacgg ctggctcctt ctcctgcaag gactgcgatg ggggctaccg gcccagcccc
     5701 ctgggtgact cctgtgaaga tgtggatgaa tgtgaagacc cccagagcag ctgcctggga
     5761 ggcgagtgca agaacactgt gggctcctac cagtgcctct gtccccaggg cttccagctg
     5821 gccaatggca ccgtgtgtga ggatgtgaat gagtgcatgg gggaggagca ctgcgcaccc
     5881 cacggcgagt gcctcaacag ccacgggtct ttcttctgtc tgtgcgcgcc tggcttcgtc
     5941 agcgcagagg ggggcaccag ctgccaggat gtggacgagt gtgccaccac agacccgtgt
     6001 gtgggagggc actgtgtcaa caccgagggc tccttcaact gtctatgtga gactggcttc
     6061 cagccctccc cagagagtgg agagtgtgtg gatattgacg agtgtgagga ctatggagac
     6121 ccggtgtgtg gcacctggaa gtgtgaaaac agccctggct cctaccgctg tgttctgggc
     6181 tgccagcctg gcttccacat ggccccgaac ggagactgca ttgacataga cgagtgcgcc
     6241 aacgacacca tgtgtggcag ccacggcttc tgtgacaaca ctgatggctc cttccgctgc
     6301 ctctgtgacc agggcttcga gatctctccc tcaggctggg actgtgtgga tgtgaacgag
     6361 tgtgagctta tgctggcggt atgtggggcc gcgctctgtg agaacgtgga gggctccttc
     6421 ctgtgcctct gtgccagtga cctggaggag tacgatgccc aggaggggca ctgccgccca
     6481 cggggggctg gaggtcagag tatgtctgag gccccaacgg gggaccatgc cccggccccc
     6541 acccgcatgg actgctactc cgggcagaag ggccatgcgc cctgctccag tgtcctgggc
     6601 cggaacacca cacaggctga atgctgctgc acccagggcg ctagctgggg agatgcctgt
     6661 gacctctgcc cgtctgagga ctcagctgaa ttcagcgaga tctgccctag tggaaaaggc
     6721 tacattcctg tggaaggagc ctggacgttt ggacagacca tgtacacaga tgcggatgag
     6781 tgtgtgatat tcgggcctgg tctctgcccg aacggccggt gcctcaacac cgtgcctggt
     6841 tatgtctgcc tgtgcaatcc cggcttccac tacgatgctt cccacaagaa gtgtgaggat
     6901 cacgatgagt gccaggacct ggcctgtgag aatggcgagt gcgtcaacac ggagggctcc
     6961 ttccactgct tctgcagccc cccgctcacc ctggacctca gccagcagcg ctgcatgaac
     7021 agcaccagca gcacggagga cctccctgac cacgacatcc acatggacat ctgctggaaa
     7081 aaagtcacca atgatgtgtg cagcgaaccc ctgcgtgggc accgcaccac ctacacggaa
     7141 tgctgctgcc aggacggcga ggcctggagc cagcagtgtg ctctgtgtcc cccgaggagc
     7201 tctgaggtct atgctcagct gtgcaacgtg gctcgcattg aggcagagcg ggaggccggg
     7261 gtccacttcc ggccaggcta tgagtatggc cccgggcccg atgacctgca ctacagcatc
     7321 tatggcccag atggggcccc cttctacaac tacctgggcc ccgaggacac cgtccctgag
     7381 cctgccttcc ccaacacagc cggtcactca gcggaccgca cacccatcct tgagtctcct
     7441 ttgcagccct cagaactcca gccccactac gtggccagcc atccagagcc cccagccggc
     7501 ttcgaagggc ttcaggcgga ggagtgcggc atcctgaacg gctgtgagaa tggccgctgt
     7561 gtgcgcgtgc gggagggcta cacctgtgac tgttttgagg gcttccagct ggatgcggcc
     7621 cacatggcct gcgtagatgt gaatgagtgt gatgacttga acgggcctgc tgtgctctgt
     7681 gtccatggtt actgcgagaa cacagagggc tcctaccgct gccactgctc cccgggatat
     7741 gtggctgagg cagggccccc ccactgcact gccaaggagg gatccgacta caaagaccat
     7801 gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa gtagcggccg
     7861 cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     7921 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg
     7981 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     8041 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     8101 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga
     8161 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga
     8221 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg
     8281 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct
     8341 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg
     8401 gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc
     8461 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag
     8521 ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc
     8581 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac
     8641 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc
     8701 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag
     8761 gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg
     8821 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc
     8881 gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac
     8941 agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag
     9001 aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg
     9061 ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct
     9121 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag
     9181 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag
     9241 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     9301 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     9361 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     9421 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     9481 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     9541 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     9601 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     9661 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     9721 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     9781 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct
     9841 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     9901 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     9961 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
    10021 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
    10081 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
    10141 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
    10201 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
    10261 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
    10321 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
    10381 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat
    10441 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
    10501 gctaattcac tcccaacgaa gataagatct gctttttgct tgtactgggt ctctctggtt
    10561 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
    10621 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
    10681 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
    10741 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
    10801 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    10861 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    10921 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
    10981 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
    11041 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac
    11101 ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
    11161 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
    11221 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    11281 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
    11341 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    11401 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    11461 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    11521 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    11581 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    11641 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    11701 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    11761 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    11821 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    11881 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    11941 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
    12001 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    12061 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    12121 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    12181 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    12241 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    12301 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    12361 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    12421 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    12481 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    12541 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    12601 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    12661 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    12721 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    12781 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    12841 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    12901 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    12961 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    13021 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    13081 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    13141 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    13201 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    13261 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
    13321 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
    13381 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
    13441 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
    13501 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    13561 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
    13621 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    13681 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    13741 cagtgccaag ctg
//
